Method and composition for inducing chondrogenesis or tenogenesis in mesenchymal stem cells
A cell medium for in vitro inducing chondrogenesis or tenogenesis in mesenchymal stem cells (MSCs). The medium is a glucose medium supplemented with at least one growth factor is chosen from the group of fibroblast growth factors (FGF) or the group of transforming growth factors (TGF), and the FGF or TGF is present in a total concentration of between 1 and 15 ng/ml. In both cases, IGF can be added to enhance the induction process. The use of the cell medium, a method for inducing isolated mesenchymal stem cells (MSCs) and a cell composition obtained by the method are also provided.
1. A method for inducing chondrogenesis in mesenchymal stem cells (MSCs), comprising culturing said MSCs in an inducing cell medium, wherein said medium comprises a Dulbecco's Modified Eagle's Medium (DMEM) low glucose medium supplemented with transforming growth factor (TGF) TGFβ3, wherein the growth factor is present at a total concentration of between 1 and 15 ng/ml in said medium, and wherein said MSCs are seeded at a density of 2 to 30×10 3 MSCs/cm 2 , and wherein said medium comprises between 10% and 30% fetal bovine serum or fetal calf serum of the total volume of the cell medium, to thereby induce the chondrogenesis.
2. The method according to claim 1 , wherein said growth factor is present in a total concentration of between 2 and 10 ng/ml.
3. The method according to claim 1 , wherein said inducing cell medium further comprises a growth factor selected from the group consisting of insulin growth factor (IGF) IGF-I in a concentration of between 10 and 225 ng/ml.
4. The method according to claim 1 , wherein said MSCs are cultured for a period of 1-7 days in inducing medium.