Composition and methods for detecting cancer
The invention relates to antibodies, which bind a peptide having a sequence selected from SEQ ID NO: 2, or a fragment or derivative of such an antibody having essentially the same antigen specificity. In particular, the antibodies suited to be used to detect, manage or monitor cancer in a subject, particularly cancer with interleukin-4 induced gene 1 (IL4I1) expressing cells. The invention also relates to kits or devices containing said antibodies, suitable for immunologic detection or reaction from any biological sample.
1. An isolated monoclonal antibody comprising:
a heavy chain comprising variable domain CDR sequences comprising:
(a) a H-CDR1 comprising the sequence of SEQ ID NO: 8,
(b) a H-CDR2 comprising the sequence of SEQ ID NO: 9 or SEQ ID NO: 18, and
(c) a H-CDR3 comprising the sequence of SEQ ID NO: 10 or SEQ ID NO: 19; and
a light chain comprising variable domain CDR sequences comprising:
(a) a L-CDR1 comprising the sequence of SEQ ID NO: 5,
(b) a L-CDR2 comprising the sequence of SEQ ID NO: 6 or SEQ ID NO: 14, and
(c) a L-CDR3 comprising the sequence of SEQ ID NO: 7 or SEQ ID NO: 15.
2. The isolated monoclonal antibody according to claim 1 , wherein:
the variable domain CDR sequences of the light chain consist of SEQ ID NO: 5 for L-CDR1, SEQ ID NO: 6 for L-CDR2 and SEQ ID NO: 7 for L-CDR3; and
the variable domain CDR sequences of the heavy chain consist of SEQ ID NO: 8 for H-CDR1, SEQ ID NO: 9 for H-CDR2 and SEQ ID NO: 10 for H-CDR3.
3. The isolated monoclonal antibody according to claim 1 , wherein:
the variable domain CDR sequences of the light chain consist of SEQ ID NO: 5 for L-CDR1, SEQ ID NO: 14 for L-CDR2 and SEQ ID NO: 15 for L-CDR3; and
the variable domain CDR sequences of the heavy chain consist of SEQ ID NO: 8 for H-CDR1, SEQ ID NO: 18 for H-CDR2 and SEQ ID NO: 19 for H-CDR3.
4. A device comprising the isolated monoclonal antibody according to claim 1 immobilized on a support.
5. The device of claim 4 , wherein the support is a membrane, a slide, a microarray, a chip, a microbead or a quartz prism.
6. A kit comprising the isolated monoclonal antibody according to claim 1 .
7. The kit according to claim 6 further comprising at least one reagent for performing or detecting an immune reaction.
8. An isolated monoclonal antibody produced from the hybridoma cell available under CNCM deposit number I-5355.