Spinning vessel systems and methods for mixing, suspending particulates, aliquoting, washing magnetic beads, and concentrating analytes
Provided herein are apparatuses and systems for mixing liquids and suspensions that include vessels with structures that improve mixing while not contacting liquid delivery components. The apparatuses and systems can include a motor drive that allows speed and directional control of rotation of the vessel. The apparatuses and systems can include one or more magnets for separating magnetic beads in a suspension. Also provided are methods using said apparatuses and systems for mixing and separation processes.
1. A system for mixing liquids and uniformly suspending particulates comprising:
at least one substantially cylindrical vessel with a center and a radius comprising a vessel opening, a vessel base interior, and a vessel base exterior, the vessel further comprising;
a plurality of side fins, the side fins comprising a side fin width and a side fin height;
a plurality of interior projections extending from the vessel base interior, the interior projections comprising an interior projection width and an interior projection height;
a plurality of circumferences, the circumferences each comprising a circumference radius;
wherein the circumferences comprise the following radii: 6.4 mm, 14.2 mm, 19.1 mm, 22.9 mm, 26.2 mm, 31.8 mm, 34.3 mm, 38.7 mm, 40.7 mm, 42.7 mm, 44.5 mm, 46.3 mm, 49.7 mm, 51.3 mm, 54.3 mm, 58.7 mm, 62.2 mm; or,
wherein the circumferences comprise the following radii: 4.5 mm, 13.50 mm, 22.50 mm, 31.50 mm;
wherein neither the side fin width nor interior projection width extends within 1.5 mm of any of the circumferences;
a drive base operably connected to the vessel base exterior;
a motor operatively connected to the drive base, wherein the motor rotates the vessel along the vessel's center; and,
a motor controller with speed and rotational direction control.
2. The system of claim 1 , wherein the vessel comprises a height and the side fin height or the interior projection height is less than 20% of the vessel height.
3. The system of claim 1 , wherein the interior projection width is at least 1 mm, at least 2 mm, at least 3 mm, or at least 4 mm.
4. The system of claim 1 , wherein the side fin width is at least 1 mm, at least 2 mm, at least 3 mm, at least 4 mm, at least 5 mm, at least 10 mm, at least 20 mm, at least 30 mm, at least 40 mm, at least 50 mm, at least 75 mm, at least 100 mm, at least 125 mm, at least 150 mm, or at least 200 mm.
5. The system of claim 1 , wherein the vessel base exterior comprises one or more engagement tabs and the drive base comprises one or more engagement slots.
6. The system of claim 1 , wherein the motor comprises direct drive or indirect drive.
7. The system of claim 1 , comprising at least four vessels.
8. The system of claim 1 , further comprising a liquid handling system comprising a plurality of pipettors.
9. The system of claim 8 , wherein none of the side fins or the interior projections contact the pipettors when the vessel is rotated.
10. A method for sampling a liquid or suspension, the method comprising:
(a) mixing a liquid in the vessel of the system of claim 1 by rotating the vessel in a clockwise direction for a first period of time, pausing the rotation for a second period of time, rotating the vessel in a counterclockwise direction for a third period of time, and pausing the rotation for a fourth period of time; and,
(b) optionally repeating step (a) a plurality of times; and
(c) removing a volume of the liquid or suspension.
11. The system of claim 1 , wherein neither side fins nor interior projections are within a 12.5 mm radius of the center of the vessel.
12. The system of claim 2 , wherein the vessel height is about 5 cm to about 150 cm.
13. The system of claim 1 , wherein the drive base comprises a proximal lip with an internal radius greater than the vessel radius.
14. The system of claim 13 , wherein the proximal lip comprises proximal lip height and the vessel comprises a vessel height, and wherein the height of the proximal lip is at least 10% of the height of the vessel height.
15. The system of claim 1 , wherein the vessel base exterior comprises a friction fit with the drive base.
16. The system of claim 1 , wherein the vessel base exterior is connected to the drive base via an O-ring, set screws, or adhesive tape.
17. The system of claim 1 , wherein the vessel comprises plastic.
18. The system of claim 1 , wherein the drive base comprises metal or plastic.
19. The system of claim 7 , comprising at least 6, at least 8, at least 10, at least 12, at least 16, at least 24, at least 32, at least 48, or at least 96 vessels.
20. The system of claim 1 , wherein the motor comprises a motor shaft and further comprising a coupling system attaching the drive base to the motor shaft.
21. The system of claim 5 , wherein said engagement tabs and engagement slots are configured to withstand the force of a sudden change from a first rotational direction to a second rotational direction.