System and method for detection of target substances
A system and method includes a test container for detecting a target substance in a consumable sample, where the test container includes a test container body defining a test container top, a test container bottom opposing the test container top, a first chamber proximal the test container top, and a second chamber proximal the test container bottom, a magnetic diaphragm situated between the first chamber and the second chamber, the magnetic diaphragm obstructing flow of the consumable sample, and the magnetic diaphragm including a magnetic element embedded in the magnetic diaphragm, and a driving element geometrically complementary to the first chamber, the driving element including a consumable sample grinding feature protruding from a surface of the driving element.
1. A test container for detecting a harmful substance, the test container comprising:
a first chamber configured to receive a sample;
a second chamber positioned below the first chamber and configured to be in fluid communication with the first chamber, the first chamber vertically aligned with the second chamber, the first chamber or the second chamber including a reagent that produces a dispersion when combined with the sample;
an analysis chamber in fluid communication with the second chamber; and
a detection substrate positioned within the analysis chamber, the detection substrate being positioned entirely outside the second chamber, the detection substrate including a test strip;
wherein the sample is configured to be placed within the first chamber, combine with the reagent in the first chamber or the second chamber, and flow from the second chamber into the analysis chamber to be detected by the detection substrate to detect the harmful substance within the sample;
wherein the detection substrate includes an active region having an antibody that is complementary with the harmful substance;
wherein the detection substrate includes a control region to provide a control readout;
wherein the detection substrate is configured to soak up a portion of the dispersion, the portion of the dispersion being positioned within the analysis chamber prior to being soaked up by the detection substrate, and
wherein the portion of the sample moves upwardly along the detection substrate via a capillary flow.
2. The test container of claim 1 , wherein the harmful substance is a food allergen.
3. The test container of claim 1 , further comprising a detection window, and
wherein the detection window facilitates observation of the active region of the detection substrate.
4. The test container of claim 1 , wherein the first chamber is in selective fluid communication with the second chamber.
5. The test container of claim 4 , wherein the first chamber includes a first opening configured to receive the sample for placement into the first chamber, and a second opening opposite the first opening, and
wherein the test container further comprises a diaphragm positioned between the first chamber and the second chamber, the diaphragm extending across the second opening.
6. The test container of claim 5 , wherein the diaphragm is configured to move between a first configuration and a second configuration,
wherein in the first configuration the diaphragm retains the sample within the first chamber, and
wherein in the second configuration the diaphragm facilitates delivery of the sample from the first chamber to the second chamber.
7. The test container of claim 6 , wherein the diaphragm is configured to break thereby transitioning the diaphragm from the first configuration to the second configuration to release the sample from the first chamber into the second chamber.
8. The test container of claim 7 , further comprising:
a body; and
a cap that is configured to engage with the body of the test container to seal the test container, and
wherein when the cap seals the test container, the diaphragm is configured to break to release the sample from the first chamber into the second chamber.
9. A test container for detecting a harmful substance, the test container comprising:
a first chamber configured to receive a sample;
a second chamber positioned below the first chamber and configured to be in fluid communication with the first chamber, the first chamber vertically aligned with the second chamber, the first chamber or the second chamber including a reagent that produces a dispersion when combined with the sample;
an analysis chamber in fluid communication with the second chamber; and
a detection substrate that includes a test strip, the detection substrate including an active region having an antibody that is complementary with the harmful substance that is a food allergen, the detection substrate positioned within the analysis chamber and entirely outside the second chamber;
wherein the sample is configured to be placed within the first chamber, combine with the reagent in the first chamber or the second chamber, and flow from the second chamber into the analysis chamber to be detected by the detection substrate to detect the harmful substance within the sample;
wherein the detection substrate is configured to soak up a portion of the dispersion from within the analysis chamber, and
wherein the portion of the sample moves upwardly along the detection substrate via a capillary flow.
10. The test container of claim 9 , further comprising a port positioned between the analysis chamber and the second chamber, the port facilitating fluid communication between the analysis chamber and the second chamber.
11. The test container of claim 9 , further comprising a cap that is configured to threadingly engage a body of the test container to seal the test container.
12. The test container of claim 11 , wherein the cap includes a shaft and a head,
wherein the shaft is configured to be inserted into the first chamber, and
wherein the head has a larger width than a width of the first chamber.
13. The test container of claim 9 , further comprising a detection window that facilitates observation of the active region of the detection substrate.
14. The test container of claim 9 , wherein the first chamber includes a reagent;
wherein the sample that includes the harmful substance is configured be placed within the first chamber, combined with the reagent in the first chamber, directed into the second chamber, and directed into the analysis chamber so that the harmful substance in the sample is detected by the detection substrate.
15. The test container of claim 9 , further comprising a diaphragm positioned between the first chamber and the second chamber; and
wherein the diaphragm is configured to break thereby transitioning the diaphragm from a first configuration that retains the sample within the first chamber to a second configuration to release the sample from the first chamber into the second chamber.
16. The test container of claim 15 , further comprising:
a body; and
a cap that is configured to engage with the body of the test container to seal the test container; and
wherein when the cap seals the test container, the diaphragm is configured to break to release the sample from the first chamber into the second chamber.
17. A test container for detecting a harmful substance, the test container comprising:
a first chamber configured to receive a sample, the first chamber including a first opening configured to receive the sample for placement into the first chamber, and a second opening opposite the first opening;
a second chamber positioned below the first chamber and configured to be in fluid communication with the first chamber, the first chamber vertically aligned with the second chamber, the first chamber or the second chamber including a reagent that produces a dispersion when combined with the sample;
a diaphragm positioned between the first chamber and the second chamber, the diaphragm extending across the second opening, the first chamber being in selective fluid communication with the second chamber;
an analysis chamber in fluid communication with the second chamber; and
a detection substrate positioned within the analysis chamber, the detection substrate being positioned entirely outside the second chamber, the detection substrate including a test strip; and
wherein the sample is configured to be placed within the first chamber, combine with the reagent in the first chamber or the second chamber, and flow from the second chamber into the analysis chamber to be detected by the detection substrate to detect the harmful substance within the sample.
18. The test container of claim 17 , wherein the diaphragm is configured to break thereby transitioning the diaphragm from a first configuration that retains the sample within the first chamber to a second configuration to release the sample from the first chamber into the second chamber.
19. The test container of claim 18 , further comprising:
a body; and
a cap that is configured to engage with the body of the test container to seal the test container; and
wherein when the cap seals the test container, the diaphragm is configured to break to release the sample from the first chamber into the second chamber breaks; and
wherein the harmful substance is a food allergen.
20. The test container of claim 1 , wherein the harmful substance is gluten.
21. The test container of claim 9 , wherein the harmful substance is gluten.
22. The test container of claim 17 , wherein the detection substrate is configured to detect the harmful substance that is gluten.
23. The test container of claim 9 , further comprising a grinder that is configured to grind the sample within the first chamber.
24. The test container of claim 9 , further comprising a magnet positioned within the second chamber that is configured to rotate within the second chamber.
25. The test container of claim 9 , wherein the test container is configured to be received within a receiving port of an analysis device.