IP Library › Granted Patent US 12,232,515
Granted Patent B2
US 12,232,515 · App. 16/707,014 · Granted Feb 25, 2025

Compositions and methods for polymer based shelf life extension

Inventors: Adam M. Behrens (Cambridge, MA); Sezin Yigit (Arlington, MA); Laith M. Abu-Taleb (Washington, DC); Nadia S. Hallaj (Cambridge, MA); Colin D. Preston (Boston, MA); Samantha E. Roman (Cambridge, MA); James L. Sugarman (Boston, MA)
Assignee: Cambridge Crops, Inc.
A23L3/3472A23B4/10A23B4/20A23B7/154A23B7/16
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Quick Facts
Patent No.
US 12,232,515
App. No.
16/707,014
Granted
Feb 25, 2025
Kind
B2
Abstract

The present disclosure relates to compositions, solutions, and methods for extending the shelf life of food compositions. For example, the disclosure is directed to food compositions or solutions comprising silk fibroin. In some instances, the compositions may be coated with a silk fibroin layer. The disclosure is also directed to methods of preparing food compositions. The methods include spray-coating, dip-coating, or mixing the food compositions with fragments of silk fibroin.

Claims (28)

1. A food composition comprising silk fibroin, wherein the silk fibroin is added to the food composition as a solution in which powdered silk fibroin has been reconstituted within a solvent; wherein

between 0.1-25% (weight/volume) of the solution is comprised of silk fibroin fragments;

between 1-10% of the silk fibroin fragments within the silk fibroin solution has a molecular weight of over 400 kDa; and

between 10-45% of the silk fibroin fragments within the silk fibroin solution has a molecular weight of over 300 kDa.

2. The food composition of claim 1 , wherein the silk fibroin is comprised within a solution deposited onto the food composition using spray-coating.

3. The food composition of claim 1 , wherein the silk fibroin is comprised within a solution deposited onto the food composition using dip-coating.

4. The food composition of claim 1 , wherein the silk fibroin is mixed into the food composition.

5. The food composition of claim 1 , wherein the silk fibroin is deposited onto the food composition and is not annealed post-deposition.

6. The food composition of claim 1 , wherein all or substantially all of an external surface of the food composition is coated with the silk fibroin.

7. The food composition of claim 1 , wherein the silk fibroin solution is deposited onto the food composition and is dried via air-drying, freeze-drying, vacuum drying, or heat-drying post-deposition.

8. The food composition of claim 6 , wherein the thickness of the silk fibroin-coated external surface is comprised of a single layer of at least 12 nm and no more than about 180 um.

9. The food composition of claim 6 , wherein the thickness of the silk fibroin-coated external surface is comprised of a plurality of silk fibroin layers, wherein thickness of at least one layer is from about 5 um to about 180 um.

10. The food composition of claim 1 , wherein the silk fibroin slows microbial growth on the food composition.

11. The food composition of claim 1 , wherein the silk fibroin is comprised within a solution and a solvent of the solution is at least one of water, an acid with a pH of under 6.0, or an alcohol.

12. The food composition of claim 1 , wherein at least one of the silk fibroin powder or the solvent contains an additive.

13. The food composition of claim 12 , wherein the additive is at least one of a sugar, a plasticizer, a crosslinking agent, a bacteria, a metal, an enzyme, a biologic, an antimicrobial, a chelator, a nutrient, a vitamin, an antioxidant, a filler, a microorganism, or a protein.

14. The food composition of claim 12 , wherein the additive is at least one of a coloring agent, a scent, a flavor, a green tea extract, a rosemary extract, a phenolic antioxidant, a catechin, an acerola extract, a chamomile extract, a Malphigia emarginata , a Camellia sinensis , an epicatechin, an epigallocatechin, a gallocatechin, or an epigallocatechin gallate.

15. The food composition of claim 1 , wherein the added silk fibroin alters gas transport properties within the food composition, thereby extending a shelf-life of the food composition.

16. The food composition of claim 1 , wherein the silk fibroin is comprised within a coating comprised of silk fibroin fragments with molecular weights ranging from 10 kDa-600 kDA.

17. The food composition of claim 1 , wherein all or substantially all of an external surface of the food composition is coated with the silk fibroin, and wherein the thickness of the silk fibroin-coated external surface is comprised of a single layer of at least 12 nm and no more than about 180 um.

18. The food composition of claim 1 , wherein between 10-35% of the silk fibroin fragments within the silk fibroin solution has a molecular weight of over 300 kDa.

19. A food composition comprising silk fibroin, wherein the silk fibroin is added to the food composition as a solution in which powdered silk fibroin has been reconstituted within a solvent; wherein

the silk fibroin solution is deposited onto the food composition using spray-coating,

the silk fibroin is not annealed post-deposition;

all or substantially all of an external surface of the food composition is coated with the silk fibroin, the silk fibroin coating being comprised of silk fibroin fragments with molecular weights ranging from 10 kDa-600 kDa and the external surface being comprised of a single layer with a thickness of at least 12 nm and no more than about 180 um;

between 0.1-25% (weight/volume) of the solution is comprised of silk fibroin fragments;

between 0.1-10% of the silk fibroin fragments within the silk fibroin solution has a molecular weight of over 400 kDa; and

between 1-45% of the silk fibroin fragments within the silk fibroin solution has a molecular weight of over 300 kDa.

Assignments (2)
SECURITY INTEREST Recorded Oct 9, 2024
From: CAMBRIDGE CROPS, INC.
To: FIRST-CITIZENS BANK & TRUST COMPANY
Reel/Frame 068850/0861 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 11, 2021
From: BEHRENS, ADAM M; YIGIT, SEZIN; ABU-TALEB, LAITH M.; HALLAJ, NADIA S.; PRESTON, COLIN D.; ROMAN, SAMANTHA E.; SUGARMAN, JAMES L.
To: MORI
Reel/Frame 057748/0220 →
Continuity (2)
Provisional Application 62777512 · Dec 10, 2018
Related Publication 20200178576A1 · Jun 11, 2020
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