Paraffin shield coating for microscope slide
A shield coating on a microscope slide, and particularly a selective application of a paraffin layer to shield biomaterials and inorganic chemical deposits from microbial attack and oxidation, and more particularly, an application of a paraffin layer on a microscope slide as an exposure shield over deposited biomaterial reactive targets. The paraffin shield layer blocks the biomaterial and chemical targets from exposure, which may lead to degradation due to oxidation and provides resistance to fungal growth, while using the existing stain processing steps to remove the paraffin from the shield and co-resident tissue section.
1. A microscope slide with a paraffin shield coating, wherein
the paraffin shield coating is coated on a biomaterial and an inorganic deposit on the microscope slide,
the biomaterial is selected from a protein, a peptide, a conjugated protein, a protein coated bead, a peptide coated bead, and a conjugated coated bead which reacts with applied antibody and secondary stain reagents, or a combination thereof, and
the inorganic deposit is selected from an inorganic target and an imaging reference target, or a combination thereof, and the inorganic deposit is located on the microscope slide separate from the biomaterial, and
the paraffin shield coating is coated on the microscope slide by following steps:
(a) melting a solid paraffin at a temperature in the range of 60 to 75° C. until the solid paraffin melts into a liquid paraffin;
(b) adding a solvent to the liquid paraffin obtained in step (a) until a saturation mixture is obtained;
(c) allowing the saturation mixture obtained in step (b) to cool in a temperature between 30 and 33° C., followed by slowly adding a solvent until a clear paraffin liquid is obtained;
(d) applying a layer of the clear paraffin liquid resultant in step (c) over the biomaterial and the inorganic deposit on the microscope slide to form the paraffin shield coating over all or a selective area of the microscope slide;
(e) applying infrared heat to evaporate the solvent from the paraffin shield coating and thereby returning it to its hardened and solid state,
the solvent is selected from xylene, Aliphatic xylene substitute, Toluene, Paint thinner, Turpentine, a 50:50 mix of Acetone & Kerosene, or a mixture of the listed solvents thereof.
2. The microscope slide as claimed in claim 1 , wherein the solid paraffin is purified and water free.
3. The microscope slide as claimed in claim 1 , wherein the solid paraffin has a melting temperature of 50-60° C.
4. The microscope slide as claimed in claim 1 , wherein the solid paraffin has a melting temperature of 50-54° C.
5. The microscope slide as claimed in claim 1 , wherein the solvent is xylene, Xylol, or Toluene.
6. The microscope slide as claimed in claim 1 , wherein the paraffin shield coating has a thickness between 1 and 5 microns.
7. The microscope slide as claimed in claim 1 , wherein the imaging reference target is a pigment colored deposit.
8. The microscope slide as claimed in claim 1 , wherein the inorganic target is a compound reacting to a stain selected from Alcian Blue, Analine Blue-Orange G Solution, Azan Stain, Bielschowsky silver stain, Brow & Benn-Gramm Stain, Cresyl Violet, DAB, Fontana Masson, Gordon and Sweet's silver staining, Grocett's Methanamine silver method, Hall's Bilirubin stain, Jones Methanamine silver, Luxol Fast Blue, Luxol Fast Blue-Cresyl Mucicarmine, Muller-Mowry colloidal Iron, Orange G, Nuclear Fast Red, PAS with Diastase Digestion, Periodic Acid Schiff (PAS), Phosphotungstic Acid, Haematoxylin, Picro Sirius Red, Toluidine Blue Acidified, Trichrome Gomoris One-Step, Trichrome-Masson's, Victoria Blue, Von Kossa, Weigert's Resorcin Fuchsin, Weigert's Iron Haematoxylin, Zell-Neelsen.
9. The microscope slide as claimed in claim 1 , wherein in step (d), the clear paraffin liquid obtained in step (c) is applied through spray application method, inkjet deposit method, transfer printing method, screen printing method, or vapor deposition method.
10. The microscope slide as claimed in claim 1 , wherein the microscope slide is either glass or plastic slide.
11. The microscope slide as claimed in claim 1 , wherein the paraffin shield coating is used to protect the biomaterial and the inorganic deposit on the microscope slide from oxidation or microbial attack and inorganics from reactions resulting in oxidation or airborne delivered acid or caustic materials.
12. The microscope slide as claimed in claim 1 , wherein the paraffin shield coating is used to increase the shelf life of the biomaterial and the inorganic deposit on the microscope slide.
13. The microscope slide as claimed in claim 6 , wherein the paraffin shield coating has a thickness between 2 to 3 microns.
14. The microscope slide as claimed in claim 7 , wherein the imaging reference target comprises black and white pigments.
15. The microscope slide as claimed in claim 1 , wherein the paraffin shield coating is removable from at least one of the biomaterial or the inorganic deposit.
16. A microscope slide with a paraffin shield coating, wherein
the paraffin shield coating is coated on a biomaterial and an inorganic deposit on the microscope slide, wherein the paraffin shield coating is removable from at least one of the biomaterial or the inorganic deposit,
the biomaterial is selected from a protein, a peptide, a conjugated protein, a protein coated bead, a peptide coated bead, and a conjugated coated bead which reacts with applied antibody and secondary stain reagents, or a combination thereof, and
the inorganic deposit is selected from an inorganic target and an imaging reference target, or a combination thereof, and
the paraffin shield coating is coated on the microscope slide by following steps:
(a) melting a solid paraffin at a temperature in the range of 60 to 75° C. until the solid paraffin melts into a liquid paraffin;
(b) adding a solvent to the liquid paraffin obtained in step (a) until a saturation mixture is obtained;
(c) allowing the saturation mixture obtained in step (b) to cool in a temperature between 30 and 33° C., followed by slowly adding a solvent until a clear paraffin liquid is obtained;
(d) applying a layer of the clear paraffin liquid resultant in step (c) over the biomaterial and the inorganic deposit on the microscope slide to form the paraffin shield coating over all or a selective area of the microscope slide;
(e) applying infrared heat to evaporate the solvent from the paraffin shield coating and thereby returning it to its hardened and solid state.
17. The microscope slide as claimed in claim 16 , wherein the biomaterial is located on the microscope slide at a location separated from the inorganic deposit.