Devices, compositions and methods for imaging with Raman scattering
Methods, systems and computer-accessible medium for imaging a living cell or a living organism with bond-edited compounds using stimulated Raman scattering are disclosed. The method comprises the steps of introducing one or more bond-edited compounds into a live cell or a living organism, and detecting a vibrational tag in the cell or organism with stimulated Raman scattering. Also disclosed are methods for detecting a disease condition in a subject, methods for monitoring treatment for a disease condition, methods for screening an agent, methods for tracing a cellular process in a live cell using bond-edited compounds in combination with stimulated Raman scattering. Also disclosed are a composition for labeling a target cell with at least one bond-edited compound and devices for imaging bond-edited compounds by stimulated Raman scattering.
1. A bond-edited compound comprising a vibrational tag, wherein the bond-edited compound exhibits at least one Raman peak in a region of 1800 cm −1 to 2800 cm −1 , wherein the vibrational tag comprises at least one 13 C atom, and wherein the bond-edited compound comprises a bond-edited dye that is conjugated to a biological molecule.
2. The bond-edited compound according to claim 1 , wherein the vibrational tag further comprises —C≡ 15 N—.
3. The bond-edited compound according to claim 1 , wherein the vibrational tag comprises —C≡ 13 C—, — 13 C≡ 3 C—, or 13 C≡N.
4. The bond-edited compound according to claim 1 , wherein the vibrational tag comprises 13 C≡ 15 N—.
5. A label, comprising:
at least one bond-edited compound that is a dye comprising a vibrational tag, wherein the bond-edited compound exhibits at least one Raman peak in a region of 1800 cm −1 to 2800 cm −1 , wherein the vibrational tag comprises —C≡ 15 N— or — 13 C≡ 15 N—, and wherein the bond-edited compound comprises a bond-edited dye that is conjugated to a biological molecule.
6. The label of claim 5 , wherein the at least one bond-edited compound further comprises at least one isotopically modified alkyne.
7. The label of claim 5 , further comprising at least one chemical.
8. The label of claim 5 , wherein the dye has a light absorption peak that is between 350 nm and 543 nm.
9. The label of claim 5 , wherein the dye has a light absorption peak that is between 650 nm and 740 nm.
10. The label of claim 5 , wherein the dye has a light absorption peak that is between 740 nm and 895 nm.
11. A label, comprising:
at least one bond-edited compound that is a dye comprising a vibrational tag, wherein the bond-edited compound exhibits at least one Raman peak in a region of 1800 cm −1 to 2800 cm −1 , wherein the label further comprises at least one light absorbing protein.
12. A label, comprising:
at least one bond-edited compound that is a dye comprising a vibrational tag,
wherein the bond-edited compound exhibits at least one Raman peak in a region of 1800 cm −1 to 2800 cm −1 ,
wherein the vibrational tag comprises —C≡ 15 N— or — 13 C≡ 15 N—, and
wherein the dye has a light absorption peak that is between 350 nm and 543 nm.
13. A label, comprising:
at least one bond-edited compound that is a dye comprising a vibrational tag,
wherein the bond-edited compound exhibits at least one Raman peak in a region of 1800 cm-1 to 2800 cm-1, wherein the vibrational tag comprises —C≡ 15 N— or — 13 C≡ 15 N— , and
wherein the dye has a light absorption peak that is between 740 nm and 895 nm.