In vitro diffuse reflectance spectroscopy
An all-purpose device capable of in vitro spectral analysis of sunscreen compositions as well as diffuse reflectance spectroscopy (DRS) capabilities on human skin includes a fiber optic guide, a light source positioned at one end of the fiber optic guide, and a sunscreen substrate assembly positioned at another end of the fiber optic guide.
1. A method of in vitro measurement of the protection of a sunscreen composition, the method comprising:
applying the sunscreen composition to a sunscreen substrate, the sunscreen substrate being positioned on top of at least a layer of gelatin or other media containing scattering elements therein, the layer of gelatin being placed on top of a layer of foil;
directing a light source at the sunscreen composition on the sunscreen substrate; and
measuring the amount of light remitted from the sunscreen.
2. The method of claim 1 , wherein the sunscreen composition contains an organic sunscreen active ingredient.
3. The method of claim 1 , wherein the sunscreen composition contains an inorganic sunscreen active ingredient.
4. The method of claim 1 , wherein the sunscreen substrate is a UVB transmissive substrate.
5. The method of claim 1 , wherein the sunscreen substrate is one of plastic food wrap, nylon film, or lambskin.
6. The method of claim 1 , wherein at least some of the light from the light source travels through the sunscreen composition, travels through the sunscreen substrate, is remitted off of the layer of gelatin or the other media containing scattering elements therein, travels back through the sunscreen substrate, travels back through the sunscreen composition, travels into an optical head of a fiber optic guide, and travels to a photosensor.
7. The method of claim 1 , wherein the sunscreen substrate is a UVA and a UVB transmissive substrate.
8. A system of in vitro measurement of the protection of a sunscreen composition, the system comprising:
a fiber optic guide;
a light emission and sensing device positioned at one end of the fiber optic guide; and
a sunscreen substrate assembly positioned at another end of the fiber optic guide, the sunscreen substrate assembly including a layer of sunscreen composition applied to a sunscreen substrate, the sunscreen substrate being positioned on top of at least a layer of gelatin or other media containing scattering elements therein, the layer of gelatin being placed on top of a layer of foil,
wherein at least some light emitted from the light emission and sensing device is remitted off of the sunscreen substrate assembly and measured by a photosensor of the light emission and sensing device.
9. The system of claim 8 , wherein the light emission and sensing device includes a xenon flash lamp.
10. The system of claim 8 , wherein the sunscreen substrate to which the layer of sunscreen composition is applied has a thickness of less than 0.73 mm.
11. The system of claim 8 , wherein the fiber optic guide includes an emitted light conduit for conducting light from the light source to the sunscreen and a remitted light conduit for conducting light remitted from the sunscreen to the photosensor.
12. The system of claim 8 , wherein the sunscreen composition contains an organic.
13. The system of claim 8 , wherein the sunscreen contains an inorganic sunscreen active ingredient.
14. The system of claim 8 , wherein the sunscreen substrate is a UVB transmissive substrate.
15. The system of claim 8 , wherein the sunscreen substrate is one of plastic food wrap, nylon film, or lambskin.
16. The system of claim 8 , wherein the sunscreen substrate is a UVA and a UVB transmissive substrate.