Granted Patent
B2
US 12,690,582 · App. 16/738,870 · Granted Jul 28, 2026
Viable cell compositions, and methods related to same
View Patent ↗
Loading inventors, assignments & file history…
Abstract
Disclosed are viable cell compositions and related methods of preparation, maintenance and use. The viable cell composition can contain specified levels of cells, hydroxyethyl starch, and dimethylsulfoxide, and can be cryopreserved. The cryopreserved form of the composition can be thawed and combined with an aqueous liquid diluting medium to prepare a diluted viable cell composition that can contain specified, reduced levels of the dimethylsulfoxide and hydroxyethyl starch. The diluting medium can contain trehalose. The prepared, diluted viable cell composition can be administered to a patient.
Claims (23)
1 . A method for treating a patient with viable uterine regenerative cells, comprising:
thawing a cryopreserved composition comprising viable uterine regenerative cells and a saline cryopreservation medium, wherein the saline cryopreservation medium of the cryopreserved composition consists of dimethylsulfoxide at a concentration of 2% and hydroxyethyl starch at a concentration of about 2% to about 10% producing a thawed viable uterine regenerative cell suspension;
combining an aqueous liquid diluting medium with the thawed viable uterine regenerative cell suspension in a volume ratio of at least 3:1 to produce a diluted viable uterine regenerative cell composition containing hydroxyethyl starch and dimethylsulfoxide, wherein the dimethylsulfoxide is present in the diluted viable uterine regenerative cell composition at a concentration of about 0.5% or less; and
administering the diluted viable uterine regenerative cell composition intravenously to the patient.
2 . The method of claim 1 , wherein the saline cryopreservation medium of the cryopreserved composition consists of dimethylsulfoxide at a concentration of 2% and hydroxyethyl starch at a concentration of about 4% to about 8%.
3 . The method of claim 1 , wherein the saline cryopreservation medium of the cryopreserved composition consists of dimethylsulfoxide at a concentration of 2% and hydroxyethyl starch at a concentration of about 6%.
4 . The method of claim 1 , wherein the viable uterine regenerative cells are viable human uterine regenerative cells.
5 . The method of claim 1 , wherein the viable uterine regenerative cells are viable canine uterine regenerative cells.
6 . The method of claim 1 , wherein the viable uterine regenerative cells are viable feline uterine regenerative cells.
7 . The method of claim 1 , further comprising, prior to thawing the cryopreserved composition, storing the cryopreserved composition at a temperature in the range of about −60° C. to about −100° C.
8 . The method of claim 1 , wherein the cryopreserved composition comprises viable uterine regenerative cells at a concentration of 10 million cells/mL.
9 . The method of claim 1 , wherein the patient is being treated for osteoarthritis.
10 . The method of claim 1 , wherein the patient is being treated for chronic renal failure disease.
11 . The method of claim 1 , wherein the patient is being treated for eczema.
12 . The method of claim 1 , wherein the patient is being treated for rheumatoid arthritis.
13 . The method of claim 1 , wherein the patient is being treated for polyarthritis.
14 . The method of claim 1 , wherein the patient is a human.
15 . The method of claim 1 , wherein the patient is a canine.
16 . The method of claim 1 , wherein the patient is a feline.
17 . The method of claim 1 , wherein the saline cryopreservation medium is a buffered saline solution.
18 . The method of claim 1 , wherein the saline cryopreservation medium is a phosphate buffered saline solution.
19 . The method of claim 1 , wherein the dimethylsulfoxide is present in the diluted viable uterine regenerative cell composition at a concentration of about 0.2%.
20 . The method of claim 1 , wherein the volume ratio of the aqueous liquid diluting medium to the thawed viable uterine regenerative cell suspension is 8:1.
Assignments (4)
SECURITY INTEREST
Recorded Oct 23, 2024
From: GALLANT PET, INC.
To: WTI FUND X, INC.; WTI FUND XI, INC.
Reel/Frame 068989/0866 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 28, 2023
From: ZACHARIAS, SHELLY J.; WOODS, ERIK J.
To: COOK GENERAL BIOTECHNOLOGY LLC
Reel/Frame 064716/0805 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 28, 2023
From: COOK REGENTEC LLC; COOK GENERAL BIOTECHNOLOGY LLC
To: GALLANT PET, INC.
Reel/Frame 064716/0823 →
SECURITY INTEREST
Recorded Jun 28, 2023
From: GALLANT PET, INC.
To: WTI FUND X, INC.
Reel/Frame 064102/0263 →
Continuity (3)
Provisional Application
62314316
· Mar 28, 2016
Related Publication
20200383317A1
· Dec 10, 2020
References Cited (388)
US 3753432A
· Guerra
· 1973
[cited by applicant]
US 3858571A
· Rudolph
· 1975
[cited by applicant]
US 3995772A
· Liautaud
· 1976
[cited by applicant]
US 4004975A
· Lionetti et al.
· 1977
[cited by applicant]
US 4292972A
· Pawelchak et al.
· 1981
[cited by applicant]
US 4347234A
· Wahlig et al.
· 1982
[cited by applicant]
US 4376634A
· Prior et al.
· 1983
[cited by applicant]
US 4404970A
· Sawyer
· 1983
[cited by applicant]
US 4412947A
· Cioca
· 1983
[cited by applicant]
US 4512342A
· Zaneveld et al.
· 1985
[cited by applicant]
US 4606337A
· Zimmermann et al.
· 1986
[cited by applicant]
US 4616998A
· Wong
· 1986
[cited by applicant]
US 4705517A
· DiPisa, Jr.
· 1987
[cited by applicant]
US 4852568A
· Kensey
· 1989
[cited by applicant]
US 4890612A
· Kensey
· 1990
[cited by applicant]
US 4948540A
· Nigam
· 1990
[cited by applicant]
US 4981465A
· Ballan et al.
· 1991
[cited by applicant]
US 5021059A
· Kensey et al.
· 1991
[cited by applicant]
US 5061274A
· Kensey
· 1991
[cited by applicant]
US 5192302A
· Kensey et al.
· 1993
[cited by applicant]
US 5222974A
· Kensey et al.
· 1993
[cited by applicant]
US 5224493A
· Sawan et al.
· 1993
[cited by applicant]
US 5268148A
· Seymour
· 1993
[cited by applicant]
US 5282827A
· Kensey et al.
· 1994
[cited by applicant]
US 5310407A
· Casale
· 1994
[cited by applicant]
US 5334216A
· Vidal et al.
· 1994
[cited by applicant]
US 5360410A
· Wacks
· 1994
[cited by applicant]
US 5374261A
· Yoon
· 1994
[cited by applicant]
US 5382261A
· Palmaz
· 1995
[cited by applicant]
US 5383886A
· Kensey et al.
· 1995
[cited by applicant]
US RE34866E
· Kensey et al.
· 1995
[cited by applicant]
US 5388588A
· Nabai et al.
· 1995
[cited by applicant]
US 5391183A
· Jansen et al.
· 1995
[cited by applicant]
US 5437631A
· Jansen
· 1995
[cited by applicant]
US 5441517A
· Kensey et al.
· 1995
[cited by applicant]
US 5449375A
· Vidal et al.
· 1995
[cited by applicant]
US 5456693A
· Conston et al.
· 1995
[cited by applicant]
US 5467780A
· Nabai et al.
· 1995
[cited by applicant]
US 5478337A
· Okamoto et al.
· 1995
[cited by applicant]
US 5486359A
· Caplan et al.
· 1996
[cited by applicant]
US 5514097A
· Knauer
· 1996
[cited by applicant]
US 5514158A
· Kanesaka
· 1996
[cited by applicant]
US 5522840A
· Krajicek
· 1996
[cited by applicant]
US 5531759A
· Kensey et al.
· 1996
[cited by applicant]
US 5533518A
· Vogler
· 1996
[cited by applicant]
US 5545178A
· Kensey et al.
· 1996
[cited by applicant]
US 5550127A
· Pantano
· 1996
[cited by applicant]
US 5571181A
· Li
· 1996
[cited by applicant]
US 5578459A
· Gordon et al.
· 1996
[cited by applicant]
US 5584827A
· Korteweg et al.
· 1996
[cited by applicant]
US 5591204A
· Janzen et al.
· 1997
[cited by applicant]
US 5635387A
· Fei et al.
· 1997
[cited by applicant]
US 5643317A
· Pavcnik et al.
· 1997
[cited by applicant]
US 5653387A
· Takayanagi et al.
· 1997
[cited by applicant]
US 5653694A
· Powles
· 1997
[cited by applicant]
US 5656036A
· Palmaz
· 1997
[cited by applicant]
US 5665114A
· Weadock et al.
· 1997
[cited by applicant]
US 5676689A
· Kensey et al.
· 1997
[cited by applicant]
US 5681334A
· Evans et al.
· 1997
[cited by applicant]
US 5701910A
· Powles
· 1997
[cited by applicant]
US 5707393A
· Kensey et al.
· 1998
[cited by applicant]
US 5709934A
· Bell et al.
· 1998
[cited by applicant]
US 5716375A
· Fowler
· 1998
[cited by applicant]
US 5725498A
· Janzen et al.
· 1998
[cited by applicant]
US 5741223A
· Janzen et al.
· 1998
[cited by applicant]
US 5746724A
· Powles et al.
· 1998
[cited by applicant]
US 5752974A
· Rhee et al.
· 1998
[cited by applicant]
US 5789147A
· Rubenstein et al.
· 1998
[cited by applicant]
US 5827199A
· Alexander
· 1998
[cited by applicant]
US 5827735A
· Young et al.
· 1998
[cited by applicant]
US 5830130A
· Janzen et al.
· 1998
[cited by applicant]
US 5837539A
· Caplan
· 1998
[cited by applicant]
US 5861004A
· Kensey et al.
· 1999
[cited by applicant]
US 5902280A
· Powles et al.
· 1999
[cited by applicant]
US 5916265A
· Hu
· 1999
[cited by applicant]
US 5928214A
· Rubenstein et al.
· 1999
[cited by applicant]
US 5935147A
· Kensey et al.
· 1999
[cited by applicant]
US 5947997A
· Pavcnik et al.
· 1999
[cited by applicant]
US 5948425A
· Janzen et al.
· 1999
[cited by applicant]
US 5955110A
· Patel et al.
· 1999
[cited by applicant]
US 6007563A
· Nash et al.
· 1999
[cited by applicant]
US 6045569A
· Kensey et al.
· 2000
[cited by applicant]
US 6090130A
· Nash et al.
· 2000
[cited by applicant]
US 6090996A
· Li
· 2000
[cited by applicant]
US 6096052A
· Callister et al.
· 2000
[cited by applicant]
US 6096080A
· Nicholson et al.
· 2000
[cited by applicant]
US 6126675A
· Shchervinsky et al.
· 2000
[cited by applicant]
US 6145688A
· Smith
· 2000
[cited by applicant]
US 6179863B1
· Kensey et al.
· 2001
[cited by applicant]
US 6183496B1
· Urbanski
· 2001
[cited by applicant]
US 6190353B1
· Makower et al.
· 2001
[cited by applicant]
US 6200336B1
· Pavcnik et al.
· 2001
[cited by applicant]
US 6206907B1
· Marino et al.
· 2001
[cited by applicant]
US 6206931B1
· Cook et al.
· 2001
[cited by applicant]
US 6245090B1
· Gilson et al.
· 2001
[cited by applicant]
US 6245527B1
· Busfield et al.
· 2001
[cited by applicant]
US 6261309B1
· Urbanski
· 2001
[cited by applicant]
US 6277140B2
· Ginn et al.
· 2001
[cited by applicant]
US 6299619B1
· Greene, Jr. et al.
· 2001
[cited by applicant]
US 6312474B1
· Francis et al.
· 2001
[cited by applicant]
US 6358284B1
· Feamot et al.
· 2002
[cited by applicant]
US 6432116B1
· Callister et al.
· 2002
[cited by applicant]
US 6432119B1
· Callister et al.
· 2002
[cited by applicant]
US 6440373B1
· Gomes
· 2002
[cited by applicant]
US 6482179B1
· Chu et al.
· 2002
[cited by applicant]
US 6537257B1
· Wien
· 2003
[cited by applicant]
US 6537807B1
· Smith et al.
· 2003
[cited by applicant]
US 6540735B1
· Ashby et al.
· 2003
[cited by applicant]
US 6569081B1
· Nielsen et al.
· 2003
[cited by applicant]
US 6659338B1
· Dittman
· 2003
[cited by applicant]
US 6777231B1
· Katz et al.
· 2004
[cited by applicant]
US 6852533B1
· Rafii et al.
· 2005
[cited by applicant]
US 6979307B2
· Beretta et al.
· 2005
[cited by applicant]
US 7029666B2
· Bruder et al.
· 2006
[cited by applicant]
US 7052907B2
· Shi et al.
· 2006
[cited by applicant]
US 7060494B2
· Bhat
· 2006
[cited by applicant]
US 7153324B2
· Case et al.
· 2006
[cited by applicant]
US 7166133B2
· Evans et al.
· 2007
[cited by applicant]
US 7175652B2
· Cook et al.
· 2007
[cited by applicant]
US 7176022B2
· Frey
· 2007
[cited by examiner]
US 7201848B2
· Antwilder et al.
· 2007
[cited by applicant]
US 7255879B2
· Hariri
· 2007
[cited by applicant]
US 7311904B2
· Hariri
· 2007
[cited by applicant]
US 7311905B2
· Hariri
· 2007
[cited by applicant]
US 7387216B1
· Smith
· 2008
[cited by applicant]
US 7456024B2
· Dahm et al.
· 2008
[cited by applicant]
US 7468276B2
· Hariri
· 2008
[cited by applicant]
US 7470537B2
· Laughlin et al.
· 2008
[cited by applicant]
US 7470538B2
· Laughlin et al.
· 2008
[cited by applicant]
US 7556801B2
· Wernet
· 2009
[cited by applicant]
US 7560280B2
· Wernet
· 2009
[cited by applicant]
US 7674434B2
· Sakal et al.
· 2010
[cited by applicant]
US 7745106B2
· Grippi et al.
· 2010
[cited by applicant]
US 7886779B2
· Smith
· 2011
[cited by applicant]
US 7914779B2
· Hariri
· 2011
[cited by applicant]
US 7955850B2
· Yu et al.
· 2011
[cited by applicant]
US 7993918B2
· Paludan et al.
· 2011
[cited by applicant]
US 8517957B2
· Decaluwe et al.
· 2013
[cited by applicant]
US 8652802B2
· Rutty
· 2014
[cited by applicant]
US 8709797B2
· Woods
· 2014
[cited by applicant]
US 8763287B2
· Hilpert
· 2014
[cited by applicant]
US 9029138B2
· Groelz
· 2015
[cited by applicant]
US 9549715B2
· Woods et al.
· 2017
[cited by applicant]
US 9554557B2
· Nehls
· 2017
[cited by applicant]
US 20010041900A1
· Callister et al.
· 2001
[cited by applicant]
US 20010045765A1
· Nelson
· 2001
[cited by applicant]
US 20020038111A1
· Alchas et al.
· 2002
[cited by applicant]
US 20030028172A1
· Epstien et al.
· 2003
[cited by applicant]
US 20030109832A1
· Rindlisbacher
· 2003
[cited by applicant]
US 20040019295A1
· Zhou et al.
· 2004
[cited by applicant]
US 20050013844A1
· Hadlock et al.
· 2005
[cited by applicant]
US 20050163750A1
· Roser et al.
· 2005
[cited by applicant]
US 20050209568A1
· Shanley
· 2005
[cited by applicant]
US 20050239897A1
· Pittenger et al.
· 2005
[cited by applicant]
US 20060134596A1
· Sjogren et al.
· 2006
[cited by applicant]
US 20060182724A1
· Riordan
· 2006
[cited by applicant]
US 20060210544A1
· Honmou et al.
· 2006
[cited by applicant]
US 20060245977A1
· Bodner
· 2006
[cited by applicant]
US 20070009490A1
· Conte et al.
· 2007
[cited by applicant]
US 20070021684A1
· Brielmeier et al.
· 2007
[cited by applicant]
US 20070148106A1
· Wertz et al.
· 2007
[cited by applicant]
US 20070178591A1
· Honmou et al.
· 2007
[cited by applicant]
US 20070270710A1
· Frass et al.
· 2007
[cited by applicant]
US 20080044313A1
· Caisley
· 2008
[cited by applicant]
US 20080095749A1
· Aggarwal et al.
· 2008
[cited by applicant]
US 20080190857A1
· Beretta et al.
· 2008
[cited by applicant]
US 20080213227A1
· Aggarwal et al.
· 2008
[cited by applicant]
US 20080241112A1
· Westenfelder
· 2008
[cited by applicant]
US 20080241113A1
· Walton et al.
· 2008
[cited by applicant]
US 20090053182A1
· Ichim et al.
· 2009
[cited by applicant]
US 20090112119A1
· Kim
· 2009
[cited by applicant]
US 20090180997A1
· Pittenger et al.
· 2009
[cited by applicant]
US 20090208463A1
· Pittenger et al.
· 2009
[cited by applicant]
US 20090214484A1
· Mironov
· 2009
[cited by applicant]
US 20090220464A1
· Aggarwal et al.
· 2009
[cited by applicant]
US 20090246181A1
· Kuroda et al.
· 2009
[cited by applicant]
US 20100040588A1
· Walton et al.
· 2010
[cited by applicant]
US 20100098672A1
· Varney et al.
· 2010
[cited by applicant]
US 20100297094A1
· Harlan et al.
· 2010
[cited by applicant]
US 20100330047A1
· Valorani
· 2010
[cited by applicant]
US 20110027238A1
· Aggarwal et al.
· 2011
[cited by applicant]
US 20110123498A1
· Westenfelder
· 2011
[cited by applicant]
US 20110129447A1
· Meretzki et al.
· 2011
[cited by applicant]
US 20110142807A1
· Pittenger et al.
· 2011
[cited by applicant]
US 20110262402A1
· Kuroda et al.
· 2011
[cited by applicant]
US 20110305673A1
· Spees
· 2011
[cited by applicant]
US 20120027730A1
· Delgado et al.
· 2012
[cited by applicant]
US 20120052049A1
· Woods et al.
· 2012
[cited by applicant]
US 20120128641A1
· Austen, Jr.
· 2012
[cited by applicant]
US 20120149108A1
· Tanabe et al.
· 2012
[cited by applicant]
US 20120201791A1
· Yoo
· 2012
[cited by applicant]
US 20120269785A1
· Woods et al.
· 2012
[cited by applicant]
US 20120269786A1
· Woods et al.
· 2012
[cited by applicant]
US 20120269787A1
· Woods et al.
· 2012
[cited by applicant]
US 20130164267A1
· Lin et al.
· 2013
[cited by applicant]
US 20160089401A1
· Woods et al.
· 2016
[cited by applicant]
US 20170014454A1
· Woods et al.
· 2017
[cited by applicant]
US 20170332623A1
· Zacharias et al.
· 2017
[cited by applicant]
US 20170336400A1
· Meng et al.
· 2017
[cited by applicant]
US 20210252155A1
· Woods et al.
· 2021
[cited by applicant]
AU 2011295954B2
· 2015
[cited by applicant]
AU 2015252071A1
· 2015
[cited by applicant]
AU 2017241626A1
· 2018
[cited by applicant]
AU 2015339886B2
· 2019
[cited by applicant]
BR 112017006533A2
· 2017
[cited by applicant]
BR 112018070084A2
· 2019
[cited by applicant]
CN 1156967A
· 1997
[cited by applicant]
CN 1786154A
· 2006
[cited by applicant]
CN 103180435A
· 2013
[cited by applicant]
CN 107106488A
· 2017
[cited by applicant]
CN 109195445A
· 2019
[cited by applicant]
DE 2903760B1
· 1980
[cited by applicant]
DE 10144127C1
· 2003
[cited by applicant]
EP 0917879A2
· 1999
[cited by applicant]
EP 1876233A1
· 2008
[cited by applicant]
EP 2191835A1
· 2010
[cited by applicant]
EP 2105498A1
· 2012
[cited by applicant]
EP 2721930A1
· 2014
[cited by applicant]
FR 2522959A1
· 1983
[cited by applicant]
JP H10192364
· 1998
[cited by applicant]
JP 2004507454A
· 2004
[cited by applicant]
JP 2008544957A
· 2008
[cited by applicant]
JP 2009242265
· 2009
[cited by applicant]
JP 2011001114
· 2011
[cited by applicant]
JP 2012500021A
· 2012
[cited by applicant]
JP 2013233102A
· 2013
[cited by applicant]
JP 2017531446A
· 2017
[cited by applicant]
JP 2019510042A
· 2019
[cited by applicant]
RU 2180529C2
· 2002
[cited by applicant]
SU 1673130A1
· 1991
[cited by applicant]
SU 1690737A1
· 1991
[cited by applicant]
SU 1718837A1
· 1992
[cited by applicant]
WO WO198700062A1
· 1987
[cited by applicant]
WO WO199206639A2
· 1993
[cited by applicant]
WO WO199426175A1
· 1994
[cited by applicant]
WO WO9533488A1
· 1995
[cited by applicant]
WO WO199719643A2
· 1997
[cited by applicant]
WO WO199822158A2
· 1998
[cited by applicant]
WO WO199825545A1
· 1998
[cited by applicant]
WO WO200013624A2
· 2000
[cited by applicant]
WO WO200032112A1
· 2000
[cited by applicant]
WO WO200032250A1
· 2000
[cited by applicant]
WO WO200032253A1
· 2000
[cited by applicant]
WO WO200072759A2
· 2000
[cited by applicant]
WO WO200074576A1
· 2000
[cited by applicant]
WO WO2001045765A1
· 2001
[cited by applicant]
WO WO2001080865A2
· 2001
[cited by applicant]
WO WO2005020823A1
· 2005
[cited by applicant]
WO WO2005030035A2
· 2005
[cited by applicant]
WO WO2005045008A1
· 2005
[cited by applicant]
WO WO2005070302A1
· 2005
[cited by applicant]
WO WO2006078034A1
· 2006
[cited by applicant]
WO WO2006079205A1
· 2006
[cited by applicant]
WO WO2006119256A2
· 2006
[cited by applicant]
WO WO2006121445A2
· 2006
[cited by applicant]
WO WO2006123699A1
· 2006
[cited by applicant]
WO WO2007002260A2
· 2007
[cited by applicant]
WO WO2007011443A2
· 2007
[cited by applicant]
WO WO2007064819A2
· 2007
[cited by applicant]
WO WO2007077560A2
· 2007
[cited by applicant]
WO WO2007090150A2
· 2007
[cited by applicant]
WO WO2007090155A1
· 2007
[cited by applicant]
WO WO2008020815A1
· 2008
[cited by applicant]
WO WO2009031606A1
· 2009
[cited by applicant]
WO WO2010056341A2
· 2010
[cited by applicant]
WO WO2010057965A1
· 2010
[cited by applicant]
WO WO2011011055A2
· 2011
[cited by applicant]
WO WO2011047345A2
· 2011
[cited by applicant]
WO WO2013023075A1
· 2013
[cited by applicant]
WO WO2013023075A4
· 2013
[cited by applicant]
WO WO2016069173A2
· 2016
[cited by applicant]
WO WO2017172679A1
· 2017
[cited by applicant]
Chen et al., Effect of DMSO Concentration, Cell Density and Needle Gauge on the Viability of Cryopreserved Cells in Three Dimensional Hyaluronan Hydrogel, Conf Proc IEEE Eng Med Biol Soc., (2013), pp. 1-11.
[cited by examiner]
Di et al. “Development and Evaluation of trehalose Contained soltuon Formula to Preserve huc-MSCs at 4C” Journal of Cellular Physiology 27:879-884, 2012.
[cited by applicant]
Halle et al., Uncontrolled-rate freezing and storae at −80 C, with only 3.5-percent DMSO in cryoprotective solution for 109 autologous peripheral blood progenitor cell transplatations, Transfusion, vol. 41, (May 2001), …
[cited by applicant]
Maruyama et al., Simplified method for cryopreservation of islets using hydroxyethyl starch and dimethyl sulfoxide as cryoprotectants, Transplantation Proceedings, vol. 36, No. 4 (May 1, 2004), pp. 1133-1134.0041-1345.
[cited by applicant]
Moroff et al., Retention of cellular properties of PBPCs following liquid storage and cryopreservation, Transfusion, vol. 44, 2004, pp. 245-252.
[cited by applicant]
Stiff et al., Autologous bone marrow transplantation using unfractionated cells cryopreserved in dimethylsulfoxide and hydroxyethyl starch without controlled-rate freezing, Blood, vol. 46, No. 2 Oct. 1, 1987, pp. 974-97…
[cited by applicant]
Stolzing et al., Hydroxyethylstarch in cryopreservation—Mechanisms, benefits and problems, Transfusion and Apheresis Science, vol. 46, No. 2, Apr. 1, 2012, pp. 137-147. 1473-0502.
[cited by applicant]
Thirumala et al., Clinical grade adult stem cell banking, Organogenesis, 5:3, 2009, pp. 143-154.
[cited by applicant]
International Application No. PCT/US2017/024411 International Search Report and Written Opinion, mailed May 12, 2017, 15 pgs.
[cited by applicant]
Barry et al., “Mesenchymal stem cells: clinical applications and biological characterization” International Journal of Biochemistry and Cell Biology 2004, 568-584.
[cited by applicant]
Bonab et al., Aging of mesenchymal stem cells in vitro. BMC Cell Biology 2006, 7:14, pp. 1-7.
[cited by applicant]
Brooke et al., Points to Consider in Designing Mesenchymal Stem Cell-Based Clinical Trials, Transfusion Medicine and Hemotherapy, Jul. 21, 2008, vol. 35, pp. 279-285.
[cited by applicant]
CEH Horse Report, A Publication of the Center for Equine Heath, US Davis School of Veterinary Medicine, Oct. 2008, vol. 26, No. 4 in 16 pages.
[cited by applicant]
Chapter 10, “The Pericardium”, Handbook of Pathology and Pathophysiology of Cardiovascular Disease, vol. 240, Springer Netherlands (2002).
[cited by applicant]
Chen et al., “Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood”, Stem Cells International, vol. 2016, Jan. 1, 2016 (Jan. 1, 2016), pp. 1-7, XP055665649, us ISSN: 1687-966X, …
[cited by applicant]
Choi et al., The Role of Mesenchymal Stem Cells in the Functional Improvement of Chronic Renal Failure , Stem Cells and Development, vol. 18, No. 3 (2009), pp. 521-529.
[cited by applicant]
Choi et al., Mesenchymal Stem Cell Therapy for Chronic Renal Failure, (2010) Expert Opinion on Biological Therapy, 10:8, 1217-1226, DOI:10.1517/14712598.2010.500284.
[cited by applicant]
Dissanayaka et al., “Characterization of dental pulp stem cells isolated from canine premolars,” J Endod. (Aug. 2011) 37(8):1074-1080.
[cited by applicant]
Endoluminal Stenting; Chapter 45, Kenneth R. Kensey; Chapter 46, Artur M Spokojny and Timothy A. Sanborn; and Chapter 48, Nicholas N. Kipshidze, Joseph B. Horn, Victor Nikolaychik and John E. Baker, edited by Ulrich Sig…
[cited by applicant]
Garcia-Pineres, Alfonso J. et al., “DNAse treatment following thawing of cryopreserved PBMC is a procedure suitable for lymphocyte functional studies”, Journal of Immunological methods, (May 17, 2006), vol. 313, No. 12,…
[cited by applicant]
Gargett et al., Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium. Biology of Reproduction 80, 1136-1145, 2009.
[cited by applicant]
Gonzalez et al., “A putative mesenchymal stem cells population isolated from adult human testes” Biochemical and Biophysical Research Communications 385 (2009) 570-575.
[cited by applicant]
Hall et al., Evaluation of the Potential Use of Adipose-Derived Mesenchymal Stromal Cells in the Treatment of Canine Atopic Dermatitis: A Pilot Study, Veterinary Therapeutics, Summer 2010, vol. 11, No. 2.
[cited by applicant]
Heeschen, C., et al. “Nicotine Stimulates Angiogenesis and Promotes Tumor Growth and Atherosclerosis”, Nature Medicine, vol. 7., No. 7, Jul. 2001, pp. 833-839.
[cited by applicant]
Himpson, Rebecca C., et al. “Histological evidence for enhanced anal fistula repair using autologous fibroblasts in a dermal collagen matrix.” Comparative Clinical Pathology, Apr. 2006, vol. 16, No. 1.
[cited by applicant]
Information from Vascular Solutions web site (www.vascularsolutions.com) on Duett sealing device; two pages,2001.
[cited by applicant]
Information from Vasoseal web site (www.vasoseal.com) on VasoSeal ES, three pages, 2001.
[cited by applicant]
Information from Vasoseal web site (www.vasoseal.com) on VasoSeal VHD, three pages, 2001.
[cited by applicant]
Information from web site (www.pbm.ct.utwente.nl/dopdrachten/wonder.htm) on Nerve Regeneration, three pages, 2001.
[cited by applicant]
Johnson, C. et al. “Matrix Metalloproteinase-9 is Required for Adequate Angiogenic Revascularization of Ischemic Tissues: Potential Role in Capillary Branching”, Circulation Research, vol. 94 (2004) pp. 262-268.
[cited by applicant]
Kee, “Arterial Puncture Site Management”, Applied Radiology, Jul. 2000, pp. 7-12.
[cited by applicant]
Khairy, G.E.A. et al., “Percutaneous obliteration of duodenal fistula”, J.R. Coll. Surg. Edinb., 45, Oct. 2000, 342-344.
[cited by applicant]
Kharaziha et al., Improvement of liver function in liver cirrhosis patients after autologous mesenchymal stem cell injection: a phase 1-11 clinical trial, European Journal of Gastroenterology & Hepatology (2009), vol. 2…
[cited by applicant]
Kraitchman et al., Dynamic imaging of Allogenic Mesenchymal Stem Cells Trafficking to Myocardia Infarction, 2005, 112:1451-1461.
[cited by applicant]
Lakota et al., “Autologous stem cell transplantation with stem cells preserved in the presence of 4.5 and 2.2% DMSO”, Correspondence, 1996, pp. 262-263.
[cited by applicant]
Lee et al., “A Long-Term Follow-Up Study of Intravenous Autologous Mesenchymal Stem Cell Transplantation in Patients with Ischemic Stroke” Stem Cells Journals, vol. 28, No. 6 (Jun. 2010), pp. 1099-1106.
[cited by applicant]
Lindner et al., Mesenchymal Stem or Stromal Cells: Toward a Better Understanding of Their Biology?, Transfus Med Hemother. [online] Mar. 15, 2010, 37(2): pp. 75-83. [retrieved on Sep. 25, 2018]. Retrieved from the inter…
[cited by applicant]
Lisle, David A., et al., “Percutaneous Gelfoam Embolication of Chronic Enterocutaneous Fistulas: Report of Three Cases”, Diseases of the Colon & Rectum, vol. 50, No. 2, Dec. 2006.
[cited by applicant]
Lu et al., Haematologica 2006; 91:1017-1026), Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials [online], 2006 [retrieved on Jun. 2…
[cited by applicant]
Maluf-Filho, F. et al., “Endoscopic Treatment of Esophagogastric Fistulae with an Acellular Matrix”, Gastrointestinal Endoscopy, Elsevier, NL, vol. 59, No. 5, Apr. 2004 (Apr. 2004), p. 151, XP004854594 abstract.
[cited by applicant]
Miklos, J.R., et al., “Rectovaginal Fistula Repair Utilizing a Cadaveric Dermal Allograft”, International Urogynecology Journal, 1999, vol. 10, No. 6, pp. 405-406.
[cited by applicant]
Moore et al., “Rectovaginal Fistula Repair Using a Porcine Dermal Graft”, Obstetrics & Gynecology, Nov. 2004, vol. 104, No. 5, Part 2, pp. 1165-1167.
[cited by applicant]
Neupane et al., Isolation and Characterization of Canine Adipose-Derived Mesenchymal Stem Cells, Tissue Engineering: Part A, vol. 14, No. 6, 2008.
[cited by applicant]
Part 17: Gastrointestinal Tract, part of the LUMEN Histology Slide Series, http://www.meddean.luc.edu/lumen/MEdEd/Histo/frames/h_fram17.html.
[cited by applicant]
R&D Systems, Stem Cell Culture Products and Protocol Guide, 2008, pp. 1-34.
[cited by applicant]
Rojewski et al., “Phenotypic Characterization of Mesenchymal Stem Cells from Various Tissues” Transfus Med Hemother 2008;35:168-184.
[cited by applicant]
Schultz, David J., et al., “Porcine Small Intestine Submucosa as a Treatment for Enterocutaneous Fistulas”, Journal of American College of Surgeons, 2002, vol. 194, No. 4, Apr. 2002, pp. 541-543.
[cited by applicant]
Schwesinger, Wyne H., “Management of Persistent Fistula After Gastrectomy?”, online question (www.medscape.com/viewarticle/432411_print), posted on May 14, 2002.
[cited by applicant]
Semenov, et al. “Multipotent Mesenchymal Stem Cells from Human Placenta: Critical Parameters for Isolation and Maintenance of Sternness after Isolation.” [online] American Journal of Obstetrics and Gynecology, vol. 202,…
[cited by applicant]
Shah, A.M. et al., “Bronchoscopic closure of bronchopleural fistula using gelfoam”, abstract. Journal of Association of Physicians of India, 2004, vol. 52 No. JUIN, pp. 508-509.
[cited by applicant]
Shaker MA, Hindy AM, Mounir RM, Geaisa KM. Egypt Dent. J. Jul. 1995; 41(3): 1237-42.
[cited by applicant]
Sheiman, Robert G., et al., “Percutaneous Treatment of a Pancreatic Fistula after Pancreaticoduodenectomy”, J Vase Interv Radial, 2001, vol. 12, No. 4, pp. 524-526.
[cited by applicant]
Shelton, Andrew A., et al., Transperineal Repair of Persistent Rectovaginal Fistulas Using an Accelular Cadaveric Dermal Grant (AlloDerm). Diseases of the Colon & Rectum, Sep. 2006, Vo .. 49, No. 9.
[cited by applicant]
Silbert et al., “Usefulness of Collagen Plugging with VasoSeal after PTCA as Compared to Manual Compression with Identical Sheath Dwell Times”, Catheterization and Cardiovascular Diagnosis, 1998, vol. 43, pp. 421-427.
[cited by applicant]
Thermo Scientific Nalgene and Nunc, Cryopreservation Guide, Jan. 2010, pp. 1-16, 06 .
[cited by applicant]
Wu C F, “Improved cryopreservation of human embryonic stem cells with trehalose”. Reproductive BioMedicine Online, Jan. 1, 2005 Elsevier, Amsterdam, NL. vol. 11, No. 6, pp. 733-739.
[cited by applicant]
Zucconi et al., Mesenchymal Stem Cells Derived From Canine Umbilical Cord Vein—A Novel Source for Cell Therapy Studies, Stem Cells and Development, vol. 19, No. 3, 2010.
[cited by applicant]
International Preliminary Report on Patentability in international application No. PCT/US2011/049977, with mailing date of Mar. 5, 2013 in 5 pages.
[cited by applicant]
Office Action in Australian Patent Application No. 2015252071, dated Dec. 23, 2016 in 5 pages.
[cited by applicant]
Office Action in Canadian Patent Application No. 2,809,272, dated Jun. 28, 2019, in 4 pages.
[cited by applicant]
Office Action in Canadian Patent Application No. 2,809,272, dated Jul. 12, 2018, in 4 pages.
[cited by applicant]
Office Action in Canadian Patent Application No. 2,809,272, dated Jun. 20, 2017, in 5 pages.
[cited by applicant]
Office Action in Canadian Patent Application No. 2,809,272, dated Sep. 22, 2020, in 5 pages.
[cited by applicant]
International Search Report of PCT/US2011/049977, dated Jan. 17, 2012 in 3 pages.
[cited by applicant]
Office Action in European Patent Application No. 11822565.5, dated Dec. 12, 2019, in 10 pages.
[cited by applicant]
Office Action in European Patent Application No. 11822565.5, dated Oct. 8, 2020, in 10 pages.
[cited by applicant]
Office Action in European Patent Application No. 11822565.5, dated Jul. 6, 2021, in 9 pages.
[cited by applicant]
Search Report in Brazilian Application No. BR 11 2013 004917 0, dated May 4, 2020, in 24 pages.
[cited by applicant]
International Preliminary Report on Patentability in international application No. PCT/US2012/050173, with mailing date of Feb. 11, 2014.
[cited by applicant]
International Search report and Written Opinion in International Application No. PCT/US2012/050173, with a mailing date of Nov. 30, 2012.
[cited by applicant]
Communication Pursuant to Article 94(3) EPC in European Application No. 12750939.6, with a mailing date of Jul. 27, 2016, in 5 pages.
[cited by applicant]
Office Action in European Patent Application No. 12750939.6, dated Mar. 18, 2019, in 5 pages.
[cited by applicant]
International Preliminary Report on Patentability in international application No. PCT/US2015/052950, with mailing date of Apr. 4, 2017.
[cited by applicant]
Office Action in Brazilian Patent Application No. BR112017006533-9, dated Jan. 21, 2021, in 12 pages.
[cited by applicant]
Office Action in Brazilian Patent Application No. BR112017006533-9, dated Feb. 2, 2021, in 1 page.
[cited by applicant]
Office Action in Brazilian Patent Application No. BR112017006533-9, dated Mar. 11, 2021, in 8 page.
[cited by applicant]
International Search Report of PCT/US2015/052950, dated May 20, 2016 in 4 pages.
[cited by applicant]
International Search Report of Chinese Patent Application No. 2015800622943, dated Sep. 29, 2015 in 2 pages.
[cited by applicant]
Office Action in Chinese Patent Application No. 2015800622943, dated Sep. 30, 2019, in 11 pages.
[cited by applicant]
Office Action in European Patent Application No. 15854446.0, dated Sep. 2, 2019, in 7 pages.
[cited by applicant]
Office Action in Japanese Patent Application No. 2017-536231, dated Aug. 6, 2019, in 15 pages.
[cited by applicant]
Office Action in Australian Patent Application No. 2017241626, dated Apr. 6, 2021, in 5 pages.
[cited by applicant]
International Preliminary Report on Patentability in international application No. PCT/US2017/024411, with mailing date of Oct. 2, 2018 in 15 pages.
[cited by applicant]
Office Action in European Patent Application No. 17716037.1, dated Feb. 25, 2020, in 17 pages.
[cited by applicant]
Office Action in European Patent Application No. 17716037.1, dated Oct. 8, 2020, in 9 pages.
[cited by applicant]
Office Action in European Patent Application No. 17716037.1, dated Aug. 20, 2021, in 5 pages.
[cited by applicant]