Trichoderma-derived selenium nanoparticles foliar fertilizer for reducing crop fungal diseases and toxin contamination
The present invention relates to a Trichoderma -derived selenium nanoparticles foliar fertilizer which reduces crop fungal diseases and toxin contamination. For the first time, a new type of agricultural composition was disclosed, which is mainly obtained by mixing Trichoderma harzianum culture with selenite, which has a good fungistatic effect. The inventors also screened and obtained a harzianum strain with excellent effect. After fermentation, mixed with selenite and post-processed, the obtained product had a significant effect on resisting harmful fungus and improving disease resistance of the plant.
1. An agriculture composition prepared by a method comprising the step of: mixing a Trichoderma harzianum culture with selenite; wherein the Trichoderma harzianum culture is the liquid Trichoderma metabolites isolated from the culture after the fermentation of Trichoderma harzianum or the supernatant isolated from the culture after the fermentation of Trichoderma harzianum ; wherein the Trichoderma harzianum is the JF309 strain deposited with the China Center for Type Culture Collection as Deposit Accession No. CCTCC M 2019066.
2. The composition according to claim 1 , wherein the selenite comprises: sodium selenite, potassium selenite or zinc selenite.
3. The composition according to claim 2 , wherein the fermentation medium comprises potato flour and glucose.
4. The composition according to claim 3 , wherein the medium comprises: 10-22 parts by weight of potato flour, 1-2.2 parts by weight of glucose, 0.0015-0.004 parts by weight of selenite, 0.004-0.015 parts by weight of ascorbic acid, 0.0001 parts by weight of Trichoderma harzianum , and the balance of water.
5. The composition according to claim 4 , wherein the fermentation process comprises:
(a) dissolving the potato flour in water, heating to boil, and filtering to obtain the filtrate;
(b) dissolving glucose in the filtrate of step (a), mixing and sterilizing to obtain a culture medium;
(c) inoculating Trichoderma harzianum strain in the culture medium of (b), with shaking at room temperature for 5±3 days to obtain a culture solution; and
(d) filtering and centrifuging the culture solution of (c) to obtain a supernatant.
6. The composition according to claim 1 , further comprising: adding a reducing agent in the mixture after the Trichoderma harzianum culture is mixed with selenite; and centrifuging to obtain a precipitate.
7. The composition according to claim 6 , wherein the reducing agent comprises: ascorbic acid or sodium citrate.
8. The composition according to claim 1 , wherein the composition is an agrochemical composition comprising the reaction product of the Trichoderma harzianum culture and the selenite as the main active components.
9. The composition according to claim 8 , wherein the composition is an agrochemical composition comprising the reaction product of the Trichoderma harzianum culture, the selenite and the reducing agent.
10. A method for the preparation of fertilizers for reducing crop fungal diseases and toxin contamination comprising: mixing the composition according to- claim 1 with an agriculturally acceptable carrier.
11. The method according to claim 10 , wherein the fungal diseases are the diseases caused by Alternaria alternata, Fusarium verticillioide, Fusarium graminearum, Alternaria alternata, Fusarium verticillioide.
12. A Trichoderma -derived selenium nanoparticles foliar fertilizer, comprising the composition according to claim 1 and an agriculturally acceptable carrier.
13. A method for preparing an agriculture composition, comprising:
(1) fermenting Trichoderma harzianum to obtain a Trichoderma harzianum culture, isolating the liquid Trichoderma metabolites or the supernatant from the culture after the fermentation of Trichoderma harzianum ; wherein the Trichoderma harzianum is the JF309 strain deposited with the China Center for Type Culture Collection as Deposit Accession No. CCTCC M 2019066; and
(2) mixing selenite with the Trichoderma harzianum culture from (1).
14. The method according to claim 13 , wherein the selenite comprises: sodium selenite, potassium selenite, or zinc selenite.
15. The method according to claim 13 , wherein the fermentation medium comprises potato flour and glucose.
16. The method according to claim 15 , wherein the medium comprises: 10-22 parts by weight of potato flour, 1-2.2 parts by weight of glucose, 0.0015-0.004 parts by weight of selenite, 0.004-0.015 parts by weight of ascorbic acid, 0.0001 parts by weight of Trichoderma harzianum , and 75-85 parts by weight of water.
17. The method according to claim 15 , wherein the fermentation process comprises:
(a) dissolving the potato flour in water, heating to boil, and filtering to obtain the filtrate;
(b) dissolving glucose in the filtrate of step (a), mixing and sterilizing to obtain a culture medium;
(c) inoculating Trichoderma harzianum strain in the culture medium of (b), with shaking at room temperature for 5±3 days to obtain a culture solution; and
(d) filtering and centrifuging the culture solution of (c) to obtain a supernatant.
18. The method according to claim 13 , further comprising: adding a reducing agent in the mixture after the Trichoderma harzianum culture is mixed with selenite; and centrifuging to obtain a precipitate.
19. The method according to claim 18 , wherein the reducing agent comprises: ascorbic acid or sodium citrate.