Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

1. A process for the preparation of preparing at least one sugar from lignocellulosic material, comprising:

a) pretreating the lignocellulosic material at a temperature of 160° C. to 200° C. at a pH of 1.0 to 2.5 for 1 to 15 minutes;

b) enzymatically hydrolysing the pretreated lignocellulosic material having a dry matter weight of 15 to 25% (w/w) at a temperature of 50° C. to 65° C. and a pH of 4 to 6 for 40 hours to 150 hours using a whole fermentation broth of a filamentous fungus, said broth comprising at least a cellobiohydrolase, an endoglucanase, a beta-glucosidase, a xylanase, a beta-xylosidase, and a lytic polysaccharide monooxygenase; and

c) optionally, recovering the at least one sugar from the enzymatically hydrolysed pretreated lignocellulosic material,

wherein the at least one sugar is selected from the group consisting of: glucose;

cellobiose; xylose; arabinose; galactose; fructose; mannose; rhamnose; ribose;

galacturonic acid; glucuronic acid; and combinations thereof.

2. A process for the preparation of a fermentation product from lignocellulosic material, comprising:

a) pretreating the lignocellulosic material at a temperature of 160° C. to 200° C. at a pH of 1.0 to 2.5 for 1 to 15 minutes;

b) enzymatically hydrolysing the pretreated lignocellulosic material having a dry matter weight of 15 to 25% (w/w) at a temperature of 50° C. to 65° C. and a pH of 4 to 6 for 40 hours to 150 hours using a whole fermentation broth of a filamentous fungus, said broth comprising at least a cellobiohydrolase, an endoglucanase, a beta-glucosidase, a xylanase, a beta-xylosidase and a lytic polysaccharide monooxygenase, to obtain a hydrolysed lignocellulosic material;

c) fermenting the enzymatically hydrolysed pretreated lignocellulosic material to produce a fermentation product; and

d) optionally, recovering the fermentation product,

wherein the fermentation product is selected from the group consisting of: arabinitol; butanol; ethanol; glycerol; methanol; 1,3-propanediol; sorbitol; xylitol; acetic acid; acetonic acid; adipic acid; ascorbic acid; acrylic acid; citric acid; 2,5-diketo-D-gluconic acid; formic acid; fumaric acid; glucaric acid; gluconic acid;

glucuronic acid; glutaric acid; 3-hydroxypropionic acid; itaconic acid; lactic acid; maleic acid; malic acid; malonic acid; oxalic acid; oxaloacetic acid; propionic acid; succinic acid; xylonic acid; acetone; aspartic acid; glutamic acid; glycine; lysine; serine; tryptophan; threonine; ethylene; a β-lactam antibiotic; a cephalosporin; pentane; hexane; heptane; octane; nonane; decane; undecane; dodecane; cyclopentane; cyclohexane; cycloheptane; cyclooctane; pentene; hexene; heptene; octane; methane; hydrogen (H 2 ); carbon dioxide (CO 2 ); carbon monoxide (CO); a protease; a cellulase; an amylase; a glucanase; a lactase; a lipase; a lyase; an oxidoreductase; a transferase; and a xylanase.

3. The process according to claim 1 , wherein oxygen is added to the pretreated lignocellulosic material during the enzymatic hydrolysis.

4. The process according to claim 1 , wherein the enzymatic hydrolysis is performed in a reactor having a volume of 10-5000 m 3 .

5. The process according to claim 1 , wherein the enzymatic hydrolysis is conducted at a temperature of 55° C. to 65° C.

6. The process according to claim 1 , wherein the lignocellulosic material is pretreated at a pH of 1.0 to 1.5.

7. The process according to claim 1 , wherein the lignocellulosic material is pretreated for 1 to 5 minutes.

8. The process according to claim 2 , wherein the fermentation is performed with a yeast that is able to convert at least one C5 sugar.

9. The process according to claim 1 , wherein the pretreating is performed in a reactor having a volume of 30-200 m 3 .

10. The process according to claim 1 , wherein the pretreating is performed in a reactor having a height to diameter ratio of 3 : 1 to 12 : 1 .

11. The process according to claim 1 , wherein the enzymatic hydrolysis is performed in a reactor having a height to diameter ratio of 2:1 to 8:1.

12. The process according to claim 2 , wherein the fermentation is performed in a reactor having a height to diameter ratio of from 2:1 to 8:1.

13. The process according to claim 1 , wherein the at least one sugar is glucose.

14. The process according to claim 1 , wherein the at least one sugar is xylose.

15. The process according to claim 1 , wherein the at least one sugar is arabinose.

16. The process according to claim 1 , wherein the at least one sugar is glucose and xylose.

17. The process according to claim 1 , wherein the at least one sugar is glucose, xylose, and arabinose.

18. The process according to claim 2 , wherein the fermentation product is ethanol.