Medical material produced using collagen and method for producing same
Disclosed is a medical material manufactured using collagen and a method of manufacturing the same. The method includes (1) preparing collagen using distilled water as a solvent, (2) filling a syringe with the prepared collagen and then spinning the collagen through a syringe needle, (3) immediately immersing the spun collagen in a cross-linking solution, which is a mixture including therein a hyperosmotic agent and a cross-linking agent mixed with each other, (4) removing and then washing the collagen after cross-linking is completed, and (5) removing and then drying the collagen after the washing is completed. When the collagen is spun and processed into the form of a thread and the spun thread is then cross-linked, the cross-linked collagen thread has increased strength compared to before cross-linking, and the shape thereof is retained in an aqueous solution.
1. A method of manufacturing a medical material using a collagen, comprising:
(1) preparing the collagen using distilled water as a solvent;
(2) filling a syringe with the prepared collagen and then spinning the collagen through a syringe needle;
(3) immediately immersing or exposing the spun collagen in or to a cross-linking solution, which is a mixture including therein a hyperosmotic agent and a cross-linking agent mixed with each other;
(4) removing and then washing the collagen after cross-linking is completed; and
(5) removing and then drying the collagen after the washing is completed,
wherein:
the hyperosmotic agent is glycerol and the cross-linking agent is glutaraldehyde, and a ratio of the glycerol and the glutaraldehyde for manufacturing the cross-linking solution is set so that the glutaraldehyde is included in an amount of 2% (v/v) based on the glycerol as the solvent.
2. A method of manufacturing a medical material using a collagen, comprising:
preparing the collagen using distilled water as a solvent;
immediately exposing the collagen to a cross-linking solution, which is a mixture including therein a hyperosmotic agent and a cross-linking agent mixed with each other; and
drying the collagen,
wherein:
the hyperosmotic agent is glycerol and the cross-linking agent is glutaraldehyde, and a ratio of the glycerol and the glutaraldehyde for manufacturing the cross-linking solution is set so that the glutaraldehyde is included in an amount of 2% (v/v) based on the glycerol as the solvent.
3. The method of claim 1 , wherein the collagen is included at a concentration of 1 to 15(%, w/v).
4. The method of claim 1 , wherein the spinning of step (2) is performed so that the collagen is spun into a thread and the spun thread has a thickness of 100 nm to 10 mm.
5. The method of claim 1 , wherein a cross-linking time is 1 minute to 48 hours.
6. The method of claim 1 , wherein the washing of step (4) includes exposure to any one selected from among PBS (phosphate-buffered saline), methanol, ethanol, distilled water, acetone, distilled water, and a physiological saline solution.
7. The method of claim 1 , wherein the drying is performed using any one selected from among freeze-drying, dry-heat drying, and natural drying.
8. The method of claim 1 , wherein the collagen is used as any one selected from among medical threads, medical films, suture threads, dental materials, supports, tissue replacement materials, tissue-restorative materials, adhesion barriers, hemostatic materials, drug delivery carriers, and stiffeners.
9. A medical material manufactured using a collagen according to the manufacturing method of claim 1 .
10. The method of claim 2 , wherein the collagen is included at a concentration of 1 to 15(%, w/v).
11. The method of claim 2 , wherein a cross-linking time is 1 minute to 48 hours.
12. The method of claim 2 , wherein the drying is performed using any one selected from among freeze-drying, dry-heat drying, and natural drying.
13. The method of claim 2 , wherein the collagen is used as any one selected from among medical threads, medical films, suture threads, dental materials, supports, tissue replacement materials, tissue-restorative materials, adhesion barriers, hemostatic materials, drug delivery carriers, and stiffeners.