SPRAY-DRIED MIXTURE OF HUMAN MILK OLIGOSACCHARIDES
Disclosed are spray-dried powders containing a mixture of structurally distinct human milk oligosaccharides, methods for the production of said spray-dried powder, its use for the manufacture of nutritional compositions, and nutritional compositions containing said spray-dried powder.
1 . A spray-dried powder comprising a mixture of structurally distinct human milk oligosaccharides.
2 . The spray-dried powder according to claim 1 , wherein the structurally distinct HMOs of the mixture are selected from the group consisting of 2′-FL, 3-FL, LNT, LNnT, LNFPI, 3′-SL, 6′-SL, LST-a, LST-b, LST-c and DSLNT.
3 . The spray-dried powder according to claim 1 , wherein said spray-dried powder further comprises at least one monosaccharide, said at least one monosaccharide being optionally selected from the group consisting of L-fucose and N-acetylneuraminic acid.
4 . The spray-dried powder according to claim 1 , wherein the spray-dried powder comprises at least 80%-wt., at least 85%-wt., at least 90%-wt., at least 93%-wt., at least 95%-wt. or at least 98%-wt. human milk oligosaccharides.
5 . The spray-dried powder according to claim 1 , wherein at least one HMO of the mixture, optionally all HMOs of the mixture, optionally the at least one monosaccharide, are present in amorphous form.
6 . The spray-dried powder according to claim 3 , wherein the at least one monosaccharide, optionally all monosaccharides are present in amorphous form.
7 . The spray-dried powder according to claim 1 , wherein the spray-dried powder contains ≤15%-wt. of water, optionally ≤10%-wt. of water, optionally ≤7%-wt. of water, optionally ≤5%-wt. of water.
8 . A process for manufacture of a spray-dried powder as defined in claim 1 , wherein the process comprises:
a) purifying at least one of the structurally distinct HMOs from a fermentation broth;
b) providing an aqueous solution containing the at least one HMO of a); and
c) subjecting the solution of b) to spray-drying.
9 . The process according to claim 8 , wherein the aqueous solution of step b) comprises at least five, optionally seven structurally distinct HMOs, said structurally distinct HMOs being optionally selected from the group consisting of 2′-FL, 3-FL, LNT, LNnT, LNFPI, 3′-SL, 6′-SL, LST-a, LST-b, LST-c and DSLNT.
10 . The process according to claim 8 , wherein the aqueous solution of b) further comprises at least one monosaccharide, said at least one monosaccharide being optionally selected from the group consisting of L-fucose and N-acetylneuraminic acid.
11 . The process according to claim 8 , wherein the purifying the at least one HMO and/or the at least one monosaccharide from a fermentation broth (a)) comprises one or more of
i) removing the microbial cells from the fermentation broth to obtain a process stream;
ii) subjecting the process stream to at least one ultrafiltration;
iii) treating the process stream at least one time with a cation exchange resin and/or at least one time with an anion exchange resin;
iv) subjecting the process stream to at least one nanofiltration and/or diafiltration;
v) subjecting the process stream to at least one electrodialysis;
vi) treating the process stream at least one time with activated charcoal; and/or
vii) subjecting the process stream at least one time to a crystallization and/or precipitation.
12 . The process according to claim 8 , wherein the aqueous solution comprises the at least one HMO, the mixture of structurally distinct HMOs, and/or the at least one monosaccharide, in an amount totaling a saccharide amount of at least 20% (w/v), 30% (w/v), 35% (w/v), and/or up to 45% (w/v), 50% (w/v), 60% (w/v).
13 . The process according to claim 8 , wherein the aqueous solution containing the at least one HMO, and optionally the at least one monosaccharide, is spray-dried at a nozzle temperature of at least 110° C., optionally at least 120° C., optionally at least 125° C., and/or less than 150° C., optionally less than 140° C. and optionally less than 135° C.
14 . The process according to claim 8 , wherein the aqueous solution containing the at least one HMO, and optionally the at least one monosaccharide, is spray-dried at an outlet temperature of at least 60° C., optionally at least 65° C., and/or less than 80° C., optionally less than 70° C.
15 . A product comprising the spray-dried powder according to claim 1 for manufacture of a nutritional composition, optionally of an infant formula.
16 . A nutritional composition comprising the spray-dried powder according to claim 1 .
17 . The nutritional composition according to claim 16 , wherein the nutritional composition further comprises at least one probiotic microorganism.