SPRAY-DRIED SIALYLLACTOSE
Disclosed is a method for the manufacture of a spray-dried powder consisting essentially of 3′-SL and/or 6′-SL, the spray-dried powder, its use for the manufacture of nutritional compositions, and nutritional compositions containing the spray-dried powder.
1 . A spray-dried powder comprising or consisting essentially of 3′-SL and/or 6′-SL which has been produced by microbial fermentation.
2 . The spray-dried powder according to claim 1 , wherein the spray-dried powder comprises at least 80%-wt., at least 85%-wt., at least 90%-wt., at least 93%-wt., at least 95%-wt. or at least 98%-wt. 3′-SL and/or 6′-SL.
3 . The spray-dried powder according to claim 1 , wherein the 3′-SL and/or 6′-SL is present in amorphous form.
4 . The spray-dried powder according to claim 1 , wherein the spray-dried powder contains comprises 15%-wt. of water, optionally 10%-wt. of water, optionally 7%-wt. of water, optionally 5%-wt. of water.
5 . The spray-dried powder according to claim 1 , wherein the spray-dried powder is free of genetically-engineered microorganisms and nucleic acid molecules derived from genetically-engineered microorganisms.
6 . A process for the manufacture of a spray-dried powder as defined in claim 1 , the process comprises:
a) purifying the 3′-SL and/or 6′-SL from a fermentation broth;
b) providing an aqueous solution containing the 3′-SL and/or 6′-SL of a); and
c) subjecting the solution of b) to spray-drying.
7 . The process according to claim 6 , wherein the purifying the 3′-SL and/or 6′-SL from a fermentation broth a) includes one or more of
i) removing the microbial cells from the fermentation broth to obtain a cleared process stream;
ii) subjecting the cleared process stream to at least one ultrafiltration;
iii) treating the cleared process stream at least one time with a cation exchange resin and/or at least one time with an anion exchange resin;
iv) subjecting the cleared process stream to at least one nanofiltration and/or diafiltration;
v) subjecting the cleared process stream to at least one electrodialysis;
vi) treating the cleared process stream at least one time with activated charcoal; and/or
vii) subjecting the cleared process stream at least one time to a crystallization and/or precipitation.
8 . The process according to claim 6 , wherein the aqueous solution contains the 3′-SL and/or 6′-SL in an amount of at least 20% (w/v), 30% (w/v), 35 (w/v), and up to 45% (w/v), 50% (w/v), 60% (w/v).
9 . The process according to claim 6 , wherein the aqueous solution containing 3′-SL and/or 6′-SL is spray-dried at a nozzle temperature of at least 110° C., optionally at least 120° C., more optionally at least 125° C., and less than 150° C., optionally less than 140° C. and more optionally less than 135° C.
10 . The process according to claim 6 , wherein the aqueous solution containing 3′-SL and/or 6′-SL is spray-dried at an outlet temperature of at least 60° C., optionally at least 65° C., and less than 80° C., optionally less than 70° C.
11 . A product comprising the spray-dried powder according to claim 1 for manufacture of a nutritional composition, optionally an infant formula.
12 . A nutritional composition containing the spray-dried powder according to claim 1 .
13 . The nutritional composition according to claim 12 , further comprising at least one additional HMO, wherein said at least one additional HMO is a neutral HMO or a sialylated HMO.
14 . The nutritional composition according to claim 12 , wherein the at least one neutral HMO is selected from the group consisting of 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose and lacto-N-fucopentaose I.
15 . The nutritional composition according to claim 12 , wherein the at least one sialylated HMO is selected from the group consisting of 3′-sialyllactose, 6′-sialyllactose, sialyllacto-N-tetraose (LST)-a, LST-b, LST-c and disialyllacto-N-tetraose.
16 . The nutritional composition according to claim 12 , wherein the nutritional composition comprises at least one probiotic microorganism.