Compounds binding to fibroblast activation protein alpha
This disclosure describes a compound that binds to fibroblast activation protein alpha (FAP), compositions including the compound, and methods of using the compound, and compositions. In some embodiments, the compound is a monoclonal antibody that binds FAP. The compound may be used, for example, as a research tool, in clinical imaging, as a diagnostic agent, or as a therapeutic agent.
1. A composition comprising a compound that binds to fibroblast activation protein alpha (FAP), wherein the compound comprises
an amino acid sequence comprising SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5; and
an amino acid sequence comprising SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8.
2. The composition of claim 1 , wherein the compound comprises an amino acid sequence comprising SEQ ID NO:1 and an amino acid sequence comprising SEQ ID NO:2.
3. The composition of claim 1 , wherein the compound comprises a monoclonal antibody.
4. The composition of claim 1 , wherein the compound comprises a humanized monoclonal antibody.
5. The composition of claim 1 , wherein the compound comprises a single-chain variable fragment (scFv).
6. The composition of claim 1 , wherein the compound comprises a label.
7. A monoclonal antibody comprising
an amino acid sequence comprising a light chain variable region, wherein the light chain variable region CDR1 sequence comprises SEQ ID NO:3, wherein the light chain variable region CDR2 sequence comprises SEQ ID NO:4, and wherein the light chain variable region CDR3 sequence comprises SEQ ID NO:5; and
an amino acid sequence comprising a heavy chain variable region, wherein the heavy chain variable region CDR1 sequence comprises SEQ ID NO:6, wherein the heavy chain variable region CDR2 sequence comprises SEQ ID NO:7, and wherein the heavy chain variable region CDR3 sequence comprises SEQ ID NO:8.
8. The monoclonal antibody of claim 7 , wherein the monoclonal antibody comprises
a light chain variable region comprising the amino acid sequence of SEQ ID NO:1; or
a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:2; or
both.
9. The monoclonal antibody of claim 7 , wherein the monoclonal antibody comprises B12 IgG.