Kit for detecting coronary artery disease comprising an MAA protein adduct and reagents that bind to antibodies
In various embodiments methods are provided for identifying a mammal having an elevated risk for an adverse cardiac event (e.g. an MI) and/or determining the prognosis for the mammal. In certain embodiments the methods comprise determining, or causing to be determined, the presence and/or level of antibodies that bind a malondialdehyde acetaldehyde adduct (MAA adduct) in a biological sample from the mammal, where an elevated level of anti-MAA adduct antibodies, as compared to the level found in a normal healthy mammal is an indicator that that said mammal has one or more atherosclerotic lesions and/or is at elevated risk for a myocardial infarction.
1. A kit for evaluating the presence of and/or prognosis for atherosclerosis in a mammal, said kit comprising packaging containing:
a malondialdehyde-acetaldehyde (MAA) protein adduct;
an anti-IgG antibody capable of specifically binding to IgG antibodies when said IgG antibodies are bound to said MAA protein adduct;
an anti-IgM antibody capable of specifically binding to IgM antibodies when said IgM antibodies are bound to said MAA protein adduct; and
an anti-IgA antibody capable of specifically binding to IgA antibodies when said IgA antibodies are bound to said MAA protein adduct.
2. The kit of claim 1 , wherein said MAA protein adduct is provided immobilized on a solid support.
3. The kit of claim 2 , wherein said solid support comprises a material selected from the group consisting of a plastic, glass, quartz, a gel, and a metal.
4. The kit of claim 2 , wherein said solid support is selected from the group consisting of a particle, a test strip, a microtiter plate, an ELISA plate, and a microfluidic channel or chamber.
5. The kit of claim 1 , further comprising instructional materials that teach the use of measurement of antibodies against a MAA protein adduct to evaluate the risk for an adverse cardiac event, and/or to determine the prognosis of a mammal, and/or to evaluate the progression of atherosclerosis in a mammal, and/or to evaluate a treatment regimen.