Polynucleotides encoding antibodies or antigen binding fragments thereof that bind pseudomonas perv
This disclosure relates to polynucleotides encoding antibodies or antigen binding fragments thereof that bind to Pseudomonas PcrV as well as vectors and host cells comprising the same.
1. An isolated polynucleotide molecule comprising a polynucleotide that encodes an isolated antibody or antigen binding fragment thereof that specifically binds Pseudomonas PcrV and comprises:
(a) a heavy chain CDR1 comprising SYAMN (SEQ ID NO:218); a heavy chain CDR2 comprising AITMSGITAYYTDDVKG (amino acids 50-66 of SEQ ID NO:264); and a heavy chain CDR3 comprising EEFLPGTHYYYGMDV (SEQ ID NO: 220); and
(b) a light chain CDR1 comprising RASQGIRNDLG (SEQ ID NO: 221); a light chain CDR2 comprising SASTLQS (SEQ ID NO: 222); and a light chain CDR3 comprising LQDYNYPWT (SEQ ID NO: 223).
2. An isolated polynucleotide molecule comprising a polynucleotide which encodes (i) the VH of amino acids 1-124 of SEQ ID NO:264, (ii) the VL of amino acids 1-107 of SEQ ID NO:263 or (iii) both the VH of amino acids 1-124 of SEQ ID NO:264 and the VL of amino acids 1-107 of SEQ ID NO:263.
3. A vector comprising the polynucleotide of claim 1 .
4. A cell comprising the polynucleotide of claim 1 .
5. A vector comprising the polynucleotide of claim 2 .
6. A cell comprising the polynucleotide of claim 2 .
7. A cell comprising the vector of claim 3 .
8. The isolated polynucleotide molecule of claim 2 , wherein (i) the VH-encoding polynucleotide comprises nucleic acids 1-372 of SEQ ID NO:266, (ii) the VL-encoding polynucleotide comprises nucleic acids 1-321 of SEQ ID NO:265, or (iii) the VH-encoding polynucleotide comprises nucleic acids 1-372 of SEQ ID NO:266 and the VL-encoding polynucleotide comprises nucleic acids 1-321 of SEQ ID NO:265.
9. An isolated polynucleotide molecule comprising the nucleic acid sequence of SEQ ID NO: 265.
10. An isolated polynucleotide molecule comprising the nucleic acid sequence of SEQ ID NO: 266.
11. A vector comprising the polynucleotide of claim 9 .
12. A vector comprising the polynucleotide of claim 10 .
13. A cell comprising the vector of claim 11 .
14. A cell comprising the vector of claim 12 .
15. A cell comprising the vector of claim 5 .
16. A cell comprising the polynucleotide of claim 9 .
17. A cell comprising the polynucleotide of claim 10 .
18. A cell comprising the polynucleotide molecule of claim 9 and a polynucleotide molecule comprising the nucleic acid sequence of SEQ ID NO:266.
19. The polynucleotide of claim 2 , wherein the polynucleotide encodes a VH of amino acids 1-124 of SEQ ID NO:264.
20. The polynucleotide of claim 2 , wherein the polynucleotide encodes the VL of amino acids 1-107 of SEQ ID NO:263.
21. A cell comprising the polynucleotide of claim 19 and a polynucleotide molecule comprising a polynucleotide which encodes the VL of amino acids 1-107 of SEQ ID NO:263.
22. The polynucleotide of claim 19 , wherein the VH-encoding polynucleotide comprises nucleic acids 1-372 of SEQ ID NO:266.
23. The polynucleotide of claim 20 , where the VL-encoding polynucleotide comprises nucleic acids 1-321 of SEQ ID NO:265.
24. A cell comprising the polynucleotide of claim 22 and a VL-encoding polynucleotide comprising nucleic acids 1-321 of SEQ ID NO:265.
25. A cell comprising an isolated polynucleotide molecule comprising a polynucleotide that encodes an isolated antibody or antigen binding fragment thereof that specifically binds Pseudomonas PcrV and comprises:
(a) a polynucleotide molecule comprising a polynucleotide that encodes a heavy chain CDR1 comprising SYAMN (SEQ ID NO:218); a heavy chain CDR2 comprising AITMSGITAYYTDDVKG (amino acids 50-66 of SEQ ID NO:264); and a heavy chain CDR3 comprising EEFLPGTHYYYGMDV (SEQ ID NO: 220); and
(b) a polynucleotide molecule comprising a polynucleotide that encodes a light chain CDR1 comprising RASQGIRNDLG (SEQ ID NO: 221); a light chain CDR2 comprising SASTLQS (SEQ ID NO: 222); and a light chain CDR3 comprising LQDYNYPWT (SEQ ID NO: 223).