CRISPR/Cas fusion proteins and systems
Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.
1. A fusion protein comprising a Streptococcus pyogenes Cas9 protein; a marker protein; at least one first linker between the Cas9 protein and the marker protein, wherein the at least one first linker comprises SEQ ID NO:35; and a first heterologous domain that is a nuclear localization signal; wherein the fusion protein is a nuclease and cleaves both strands of a double-stranded sequence, is a nickase and cleaves one strand of a double-stranded sequence, or has no nuclease or nickase activity.
2. The fusion protein of claim 1 , wherein the marker protein is at the C-terminus of the fusion protein.
3. The fusion protein of claim 1 , further comprising an optional second linker,
wherein the nuclear localization signal, the marker protein, the first linker, the optional second linker (if present), and the Cas9 protein are arranged in the following order (N-terminus to C-terminus):
marker protein-first linker-nuclear localization signal-Cas9 protein;
marker protein-nuclear localization signal-first linker-Cas9 protein;
nuclear localization signal-marker protein-first linker-Cas9 protein;
marker protein-first linker-nuclear localization signal-first linker-Cas9 protein; or
nuclear localization signal-second linker-marker protein-first linker-Cas9 protein.
4. The fusion protein of claim 3 , wherein the nuclear localization signal, the marker protein, the first linker, and the Cas9 protein are arranged in the following order (N-terminus to C-terminus):
marker protein-first linker-nuclear localization signal-Cas9 protein.
5. The fusion protein of claim 1 , further comprising at least one additional heterologous domain other than the first heterologous domain that is a nuclear localization signal, wherein the at least one additional heterologous domain is a cell-penetrating domain, a chromatin modulating motif, an epigenetic modification domain, a transcriptional regulation domain, an RNA aptamer binding domain, or combination thereof.
6. The fusion protein of claim 1 , wherein the marker protein has an amino acid sequence comprising SEQ ID NO:19 or 20.
7. The fusion protein of claim 1 , wherein the marker protein has an amino acid sequence consisting of SEQ ID NO:19 or 20.
8. The fusion protein of claim 1 , wherein the fusion protein has an amino acid sequence comprising SEQ ID NO:48, 49, or 50.