Elite event canola NS-B50027-4
The present embodiments relate to elite event NS-B50027-4, seeds and oils obtained from NS-B50027-4, progeny derived from NS-B50027-4, the genetic and phenotypic characteristics of NS-B50027-4, and compositions and methods for the identification of elite event NS-B50027-4. In particular, NS-B50027-4 is a transgenic canola line capable of producing at least 5% DHA in its seed oil.
1. A method of producing a seed oil comprising docosahexaenoic acid (DHA), said method comprising the steps of:
(a) obtaining the seed of the inbred canola line NS-B50027-4 or its progeny, representative seed of said inbred canola line NS-B50027-4 having been deposited under ATCC Accession No. PTA-123186, wherein said NS-B50027-4 or its progeny comprises in its genome a chromosome A02 transgenic locus and a chromosome A05 transgenic locus that are characteristic of the inbred canola line NS-B50027-4; and
(b) extracting the seed oil from said seed.
2. The method of claim 1 , wherein the ratio of ω-3:ω-6 fatty acids in said oil is in the range of about 3.6 to about 6.
3. The method of claim 1 , further comprising the step of producing a foodstuff that comprises the seed oil for human consumption.
4. The method of claim 3 , wherein said foodstuff is cooking oil or salad oil.
5. The method of claim 1 , further comprising the step of producing a foodstuff that comprises the seed oil for animal consumption.
6. The method of claim 5 , wherein said foodstuff is an aquaculture feed.
7. The method of claim 1 , further comprising the step of producing a nutritional supplement that comprises the seed oil.
8. The method of claim 7 , wherein the seed oil is a refined oil.
9. The method of claim 1 , wherein said seed oil comprises about 5% to about 16% (wt. % of total fatty acids in oil), inclusive, docosahexaenoic acid (DHA).
10. The method of claim 1 , wherein said seed oil comprises about 5% to 17%, inclusive, long-chain polyunsaturated fatty acids (sum eicosapentaenoic acid, docosapentaenoic acid, and DHA as wt. % of total fatty acids in said oil).
11. A method of producing a seed meal obtained from seed of the inbred canola line NS-B50027-4 or its progeny, representative seed of said inbred canola line having been deposited under ATCC Accession No. PTA-123186, said method comprises the steps of:
(a) crushing the seeds of said inbred canola line NS-B50027-4 or its progeny;
(b) extracting the oil from the crushed seeds; and
(c) obtaining the seed meal from the crushed seeds,
wherein said NS-B50027-4 or its progeny comprises in its genome a chromosome A02 transgenic locus and a chromosome A05 transgenic locus that are characteristic of the inbred canola line NS-B50027-4.
12. The method of claim 11 , further comprising the step of producing a foodstuff that comprises the seed meal for animal consumption.
13. The method of claim 12 , wherein said foodstuff is aquaculture feed.