IP Library › Granted Patent US 12,458,499
Granted Patent B2
US 12,458,499 · App. 16/815,650 · Granted Nov 4, 2025

Three-dimensionally printed tissue engineering scaffolds for tissue regeneration

Inventors: Nathan Jonathan Castro (Long Beach, CA); Benjamin Blair Holmes (Washington, DC)
Assignee: Nanochon, Inc.
A61F2/30942A61F2/30771A61L27/12A61L27/18A61L27/48A61L27/54A61L27/56A61L27/58B29C64/30B33Y10/00B33Y80/00C08J9/26A61F2002/2835A61F2002/30766A61F2002/30784A61F2002/30838A61F2002/3084A61F2002/3092A61F2002/3093A61F2002/30948A61F2002/30968A61F2002/3097A61F2002/30971A61F2002/30985A61L2400/08A61L2400/12A61L2430/02A61L2430/06B29C64/118B29K2071/02B29K2075/00B29K2995/0056B29L2031/7532C08J9/0061C08J9/0071C08J2201/0464C08J2205/04C08J2205/042C08J2205/044C08J2205/06C08J2207/10C08J2325/06C08J2367/04C08J2375/04C08J2405/04C08J2429/04C08J2471/02C08J2489/00
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Quick Facts
Patent No.
US 12,458,499
App. No.
16/815,650
Granted
Nov 4, 2025
Kind
B2
Abstract

The present disclosure relates to a three-dimensionally (3D) printed tissue engineering scaffold for tissue regeneration and a method for manufacturing the 3D printed tissue engineering scaffold. The 3D printed tissue engineering scaffold may be fabricated at least in part from a composite material having an insoluble component and soluble component. The three-dimensional tissue scaffolds of the disclosure may be fabricated via a rapid prototyping machine. In some instances, the three-dimensional shape of the fabricated tissue engineering scaffold may correspond to a three-dimensional shape of a tissue defect of a patient.

Claims (23)

1 . A three-dimensional tissue scaffold comprising:

two or more layers of a material,

wherein the three-dimensional tissue scaffold comprises a plurality of pores having an average pore width,

wherein a surface of the two or more layers of the material comprise a plurality of pits having an average width that is below a resolution of a rapid prototyping technology,

wherein each of the two or more layers comprise a plurality of fibers of the material, and

wherein the plurality of pits are along a surface of the plurality of fibers.

2 . The three-dimensional tissue scaffold of claim 1 , wherein the plurality of pits have an average width of about 200 nm to about 50 μm.

3 . The three-dimensional tissue scaffold of claim 1 , wherein the material is an insoluble component and the plurality of pits on the surface of the two or more layers of the material correspond to an absence of a soluble component.

4 . The three-dimensional tissue scaffold of claim 1 , wherein the material is an insoluble component that remains after a soluble component of a composite material comprising the insoluble component and the soluble component is dissolved by a solvent.

5 . The three-dimensional tissue scaffold of claim 4 , wherein the plurality of pits on the surface of the two or more layers of the material correspond to an absence of the soluble component.

6 . The three-dimensional tissue scaffold of claim 4 , wherein the soluble component is polyvinyl alcohol (PVA).

7 . The three-dimensional tissue scaffold of claim 4 , wherein the insoluble component is thermoplastic polyurethane (TPU).

8 . The three-dimensional tissue scaffold of claim 4 , wherein the solvent is water.

9 . The three-dimensional tissue scaffold of claim 4 , wherein the composite material comprises from about 50 wt % insoluble component to about 95 wt % insoluble component.

10 . The three-dimensional tissue scaffold of claim 4 , wherein the composite material comprises from about 5 wt % soluble component to about 50 wt % soluble component.

11 . The three-dimensional tissue scaffold of claim 1 , wherein the two or more layers form a porous interconnecting fiber structure.

12 . The three-dimensional tissue scaffold of claim 1 , wherein the plurality of fibers of the material are fabricated using at least one rapid prototyping technology.

13 . The three-dimensional tissue scaffold of claim 12 , wherein the at least one rapid prototyping technology is selected from the group consisting of: stereolithography (SLA), digital light processing (DLP), fused deposition modeling (FDM), selective laser sintering (SLS), selective laser melting (SLM), electron beam melting (EBM), laminated object manufacturing (LOM), bio-plotting, and deposition printing.

14 . The three-dimensional tissue scaffold of claim 1 , wherein the three-dimensional tissue scaffold corresponds to the size and shape of a tissue defect.

15 . The three-dimensional tissue scaffold of claim 14 , wherein the tissue defect is a critical-sized tissue defect.

16 . The three-dimensional tissue scaffold of claim 14 , wherein the tissue defect is an osteochondral defect.

17 . The three-dimensional tissue scaffold of claim 1 , wherein the average pore width corresponds to a tissue type.

18 . The three-dimensional tissue scaffold of claim 17 , wherein the tissue type is selected from the group consisting of: bone, cartilage, connective tissue, and skeletal tissue.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 13, 2022
From: CASTRO, NATHAN JONATHAN; HOLMES, BENJAMIN BLAIR
To: NANOCHON, LLC
Reel/Frame 061411/0923 →
CHANGE OF NAME Recorded Oct 5, 2021
From: NANOCHON, LLC
To: NANOCHON, INC.
Reel/Frame 057714/0101 →
Continuity (3)
Continuation 15671048 · Aug 7, 2017
Provisional Application 62371805 · Aug 7, 2016
Related Publication 20210022871A1 · Jan 28, 2021
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