Tunable nanoscale cages from self-assembling DNA and protein building blocks
View Patent ↗Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.
1. A three-dimensional nanocage comprising:
a protein covalently linked to a polynucleotide handle, wherein the protein is a homotrimer;
wherein the polynucleotide handle is covalently linked to a solvent exposed cysteine by a disulfide linkage or
wherein the polynucleotide handle is covalently linked to a surface 4-azidophenylalanine residue; and
wherein the polynucleotide handle is at least 15 base pairs long; and
a DNA assembly comprising a polynucleotide arm complementary to the polynucleotide handle linked to the protein, wherein the polynucleotide handle and the polynucleotide arm form a double-stranded complex linked to the protein to the DNA assembly; and
wherein the DNA assembly comprises 4 oligonucleotides.
2. The nanocage of claim 1 , wherein the protein is a multimeric protein.
3. The nanocage of claim 1 , wherein each monomer of the multimeric protein is covalently linked to a polynucleotide handle.
4. The nanocage of claim 1 , wherein the DNA assembly comprises at least 5 oligonucleotides.
5. The nanocage of claim 1 , additionally comprising a payload molecule.
6. The nanocage of claim 5 , wherein the payload molecule is selected from the group consisting of a nucleic acid, a polypeptide, a protein, an enzyme, an antibody, a phospholipid, a carbohydrate, and a polysaccharide.