Modulated nutritional quality traits in seeds
This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.
1. A synthetic composition, comprising a soybean plant seed and an endophyte heterologously disposed to the soybean seed, wherein the endophyte is of the genus Curvularia that is heterologous to the soybean seed and comprises a polynucleotide sequence having at least 97% nucleotide sequence identity to the nucleotide sequence as set forth in SEQ ID NO: 31, and wherein the endophyte is manually or mechanically disposed to the soybean seed in an amount effective to colonize a soybean plant germinated from the synthetic composition.
2. The synthetic composition of claim 1 , wherein a soybean seed obtained from the colonized soybean plant exhibits an increase in crude fat content as compared to a reference soybean seed obtained from a reference soybean plant lacking the heterologous endophyte.
3. The synthetic composition of claim 1 , wherein the endophyte comprises a polynucleotide sequence having 100% nucleotide sequence identity to the nucleotide sequence as set forth in SEQ ID NO: 31.
4. The synthetic composition of claim 1 , wherein the endophyte is a Curvularia spicifera as deposited under NRRL Culture Deposit No. NRRL-67467.