Gene for induction of parthenogenesis, a component of apomictic reproduction
View Patent ↗Methods and compositions disclosed herein generally relate to genes involved in plant reproduction and methods of using the same.
1. A method of propagating one or more gametophytic cells in an ovule of a plant in the absence of egg cell fertilization, the method comprising:
transforming a plant with an ASGR-BBML gene construct comprising a nucleic acid encoding a polypeptide having at least 75% sequence similarity to the polypeptide of SEQ ID NO: 4, wherein the nucleic acid is operably-linked to an egg-cell specific promoter;
and
growing and selecting a progeny plant from the one or more gametophytic cells, wherein the progeny plant contains one or more sets of chromosomes from the transformed plant, wherein propagation of the plant occurs in the absence of egg cell fertilization.
2. The method of claim 1 , wherein the ASGR-BBML gene construct further comprises one or more untranslated region (UTR).
3. The method of claim 2 , wherein the ASGR-BBML gene construct further comprising one or more UTR comprising SEQ ID NO: 1.
4. The method of claim 1 , wherein the promoter comprises SEQ ID NO: 5.
5. The method of claim 2 wherein the ASGR-BBML gene construct has at least 70% sequence identity to SEQ ID NO: 3 or a fully complementary strand thereof.
6. The method of claim 1 , wherein an embryo is formed from an unreduced egg.
7. The method of claim 1 , wherein an embryo is formed from a somatic cell.
8. The method of claim 1 in which a polyploid plant is transformed to produce a diploid or dihaploid progeny plant.
9. The method of claim 1 , in which a diploid plant is transformed to produce a haploid progeny plant.
10. The method of claim 9 , in which the haploid progeny plant is treated to achieve chromosome doubling and production of a homozygous plant.
11. The method of claim 1 , wherein the progeny plant is obtained via culturing.
12. The method of claim 1 , wherein the plant is a monocot.
13. The method of claim 1 , wherein the plant is a dicot.
14. The method of claim 1 , wherein the plant comprises a grass or a leguminous plant.
15. The method of claim 14 , wherein the grass is a species of millet, rice, maize, wheat, sorghum, or switchgrass.
16. The method of claim 1 , wherein the plant is heterozygous and is transformed to produce a clonal offspring.
17. The method of claim 1 , wherein the plant is heterozygous and is transformed to produce a haploid offspring.
18. The method of claim 1 , wherein the method is used to propagate one or more heritable traits in the plant.
19. A plant or plant part produced by the method of claim 1 .
20. A method of producing a plant capable of being reproduced in the absence of egg cell fertilization, the method comprising:
transforming a plant with an ASGR-BBML gene construct encoding a polypeptide having at least 75% sequence similarity to the polypeptide of SEQ ID NO: 4, thereby producing a plant capable of being reproduced in the absence of egg cell fertilization.