Surface stabilization of biosensors
A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
1. A method of preparing a sensor apparatus, the method comprising:
applying a cleaning solution through a flow cell over a semiconductor device the semiconductor device including an array of field effect transistor (FET) sensors, and an array of wells formed over the array of FET sensors; the array of wells and array of FET sensors forming a sensor chip with a sensor chip surface;
applying a saccharide solution through the flow cell, the solution including a saccharide; and
removing the saccharide solution from the flow cell, a layer of the saccharide remaining over the sensor chip surface.
2. The method of claim 1 , wherein applying the cleaning solution includes flowing a basic solution through the flow cell prior to applying the saccharide solution.
3. The method of claim 1 , wherein applying the cleaning solution includes flowing a non-aqueous surfactant solution through the flow cell prior to applying the saccharide solution.
4. The method of claim 1 , wherein the saccharide includes a monosaccharide, a disaccharide, a polysaccharide, a derivation thereof, or a combination thereof.
5. The method of claim 1 , wherein the saccharide solution includes the saccharide in a range of 5 wt % to 30 wt %.
6. The method of claim 1 , wherein the saccharide solution includes a surfactant in a range of 0.01 wt % to 10.0 wt %.
7. The method of claim 2 , wherein flowing a basic solution through the flow cell comprises flowing a sodium hydroxide solution through the flow cell.
8. The method of claim 3 , wherein the non-aqueous surfactant solution includes a sulfonic acid surfactant in a non-aqueous solvent.
9. The method of claim 8 , wherein the sulfonic acid surfactant includes dodecyl sulfonic acid, dioctyl sulfosuccinic acid, or dodecyl benzene sulfonic acid, and salts thereof.
10. The method of claim 8 , wherein the non-aqueous surfactant solution is 5 wt % dodecyl benzene sulfonic acid in undecane.
11. The method of claim 4 , wherein the monosaccharide includes glucose, fructose, or galactose.
12. The method of claim 4 , wherein the disaccharide includes sucrose, trehalose, maltose, or lactose.
13. The method of claim 6 , wherein the surfactant includes a non-ionic fluorosurfactant, an alkyl polysaccharide surfactant, an alkyl sulfonate surfactant or a zwitterionic surfactant.
14. The method of claim 6 , wherein the saccharide solution including the surfactant is a sucrose solution in a range of 10 wt % to 20 wt % sucrose with an alkyl polysaccharide surfactant in a range of 0.05 wt % to 0.1 wt %.
15. The method of claim 6 , wherein the saccharide solution including the surfactant is a sucrose solution in a range of 10 wt % to 20 wt % sucrose with a zwitterionic surfactant in a range of 0.1 wt % to 0.2 wt %.
16. The method of claim 1 , wherein the array of FET sensors is an array of chemical FET (chemFET) sensors.
17. The method of claim 16 , wherein the array of chemFET sensors is an array of ion selective FET (ISFET) sensors.