Detection of colorectal cancer
The present disclosure provides, among other things, methods for colorectal cancer detection (e.g., screening) and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer detection (e.g., screening) that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR.
1. A method of screening for colorectal cancer, the method comprising:
determining a methylation status for each of the following, in deoxyribonucleic acid (DNA) of a human subject:
(a) a methylation locus within gene ZNF132;
(b) a first methylation locus within gene ADAMTS2; and
(c) a second methylation locus within gene ADAMTS2; and
diagnosing colorectal cancer in the human subject based on said determined methylation statuses.
2. The method of claim 1 , further comprising determining a methylation status for each of the following, in the DNA of the human subject:
(d) a methylation locus within gene ZNF542; and
(e) a methylation locus within gene LONRF2.
3. The method of claim 2 , further comprising determining a methylation status for a methylation locus within gene ZNF492 in the DNA of the human subject.
4. The method of claim 1 , wherein the methylation locus within gene ZNF132 comprises ZNF132 '415 (SEQ ID NO: 40).
5. The method of claim 4 , wherein the first methylation locus within gene ADAMTS2 comprises ADAMTS2 '254 (SEQ ID NO: 21).
6. The method of claim 5 , wherein the second methylation locus within gene ADAMTS2 comprises ADAMTS2 '284 (SEQ ID NO: 22).
7. The method of claim 2 , wherein the methylation locus within gene ZNF542 comprises ZNF542 '502 (SEQ ID NO: 35).
8. The method of claim 2 , wherein the methylation locus within gene LONRF2 comprises LONRF2 '281 (SEQ ID NO: 19).
9. The method of claim 3 , wherein the methylation locus within gene ZNF492 comprises ZNF492 '069 (SEQ ID NO: 42).
10. The method of claim 1 , wherein the DNA is isolated from blood or plasma of the human subject.
11. The method of claim 1 , wherein the DNA is cell-free DNA of the human subject.
12. The method of claim 1 , wherein methylation status is determined using quantitative polymerase chain reaction (qPCR).