Lipid composition for the delivery of therapeutic cargos
A construct includes a core comprising an external surface and a plurality of pores, a cargo disposed in a pore of the plurality of pores, the cargo comprising a CRISPR Cas9 component or a nucleic acid sequence encoding a CRISPR Cas9 component; and a coating coupled to the core, wherein the coating comprises a cationic lipid, a pegylated lipid, a zwitterionic lipid, and a sterol. The coating may comprise a molar ratio of about 1 cationic lipid to 1 zwitterionic lipid to 0.9 sterol to 0.15 PEGylated lipid, wherein each molar ratio optionally varies by about plus or minus 10%. A method of treatment is also provided.
1. A construct comprising:
a core comprising an external surface and a plurality of pores, wherein an average dimension of the plurality of pores is greater than about 2 nm;
a cargo disposed in a pore of the plurality of pores, the cargo comprising a CRISPR Cas9 component, or a nucleic acid sequence encoding a CRISPR Cas9 component; and
a coating coupled to the core, wherein the coating comprises a cationic lipid, a PEGylated lipid, a zwitterionic lipid, and a sterol.
2. The construct of claim 1 , wherein the core is a stellate mesoporous silica nanoparticle.
3. The construct of claim 1 , wherein the average diameter of the plurality of pores is of from about 3 nm to about 20 nm as determined by porosimetry with nitrogen adsorption-desorption analysis.
4. The construct of claim 1 , wherein the core has an average diameter of about 75 to about 400 nm.
5. The construct of claim 2 , wherein the mesoporous silica nanoparticle is monodisperse in particle diameter, wherein about 90% of the distribution lies within about 5% of the median diameter, as measured by dynamic light scattering.
6. The construct of claim 1 , wherein the coating comprises about 10 to about 50 mol % of the cationic lipid and zwitterionic lipid, about 5 to about 45 mol. % of the sterol, and about 2 to 8 mol. % of the PEGylated lipid.
7. The construct of claim 1 , wherein the coating comprises about 20 to about 40 mol % of the cationic lipid and zwitterionic lipid, about 10 to about 35 mol. % of the sterol, and about 2.5 to 6 mol. % of the PEGylated lipid.
8. The construct of claim 1 , wherein the coating comprises a molar ratio of about 1 cationic lipid to 1 zwitterionic lipid to 0.9 sterol to 0.15 PEGylated lipid, wherein each molar ratio optionally varies by about plus or minus 10%.
9. The construct of claim 1 , wherein the cationic lipid is 1,2-dioleoyl-3-trimethylammonium-propane, the zwitterionic lipid is 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, the sterol is cholesterol, and the PEGylated lipid is 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(polyethylene glycol)-2000].
10. The construct of claim 8 , wherein the cationic lipid is 1,2-dioleoyl-3-trimethylammonium-propane, the zwitterionic lipid is 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, the sterol is cholesterol, and the PEGylated lipid is 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(polyethylene glycol)-2000].
11. The construct of claim 1 , wherein the sterol is selected from the group consisting of: cholesterol, desmosterol, diplopterol, cholestanol, cholic acid, 12-deoxycholic acid, 7-deoxycholic acid, or a derivative thereof, and mixtures thereof and conjugated forms thereof.