DRY MATRIX FOR EMBEDDING VIABLE ESCHERICHIA COLI, METHOD OF MAKING SAME AND USE THEREOF
There is provided viable Escherichia coli ( E. coli ) embedded in a matrix, wherein said matrix has a water activity (a w )≤0.3, and wherein said matrix comprises a hydrocolloid-forming polysaccharide, a second polysaccharide which is different from the first polysaccharide, and a disaccharide which includes sucrose, trehalose, or a combination thereof. There is also provided methods of making same and use thereof.
1 . A particle comprising:
a first composition including a first polysaccharide in the form of a matrix and a viable Escherichia coli ( E. coli ), wherein the viable E. coli is embedded in the matrix, and wherein the first polysaccharide is a hydrocolloid-forming polysaccharide, and
a second composition free of the first polysaccharide, the second composition including a second polysaccharide and a disaccharide,
wherein the particle has a water activity (a w )≤0.3.
2 . The particle of claim 1 , wherein the first polysaccharide includes alginate.
3 . The particle of claim 1 , wherein 0.04≤a w ≤0.3.
4 . The particle of claim 1 , wherein said second composition comprises a ratio disaccharide/second polysaccharide of less than 10, wherein the ratio is wt. %/wt. %.
5 . The particle of claim 4 , wherein the ratio is of less than 5.
6 . The particle of claim 4 , wherein the ratio is of about 1.
7 . The particle of claim 1 , wherein said second composition further comprises a salt of L-glutamic acid.
8 . The particle of claim 7 , wherein said salt is sodium salt of L-glutamic acid.
9 . The particle of claim 1 , wherein said second polysaccharide includes maltodextrin.
10 . The particle of claim 1 , wherein said second polysaccharide includes dextran.
11 . The particle of claim 1 , wherein said E. coli includes a non-pathogenic E. coli.
12 . The particle of claim 1 , wherein said non-pathogenic E. coli includes the strain deposited at the International Depository Authority of Canada (IDAC) on Jan. 21, 2005, under accession number IDAC 210105-01.
13 . The particle of claim 1 , wherein said non-pathogenic E. coli includes the strain deposited at the International Depository Authority of Canada (IDAC) on Jun. 20, 2013, and attributed accession number 200613-01.
14 . The particle of claim 1 , wherein said hydrocolloid-forming polysaccharide is present in the matrix in percent by weight of total dry matter at a value of from 0.1% to 20%.
15 . The particle of claim 1 , wherein said hydrocolloid-forming polysaccharide is present in the matrix in percent by weight of total dry matter at a value of from 1 to 12%.
16 . The particle of claim 1 , wherein the first composition forms a core and the second composition is deposited on the core.
17 . The particle of claim 1 , wherein said second polysaccharide is dextran and said disaccharide is sucrose, present in a ration dextran:sucrose of from 10:1 to 1:10.
18 . A particle comprising:
a first composition including a first polysaccharide in the form of a matrix and a viable Escherichia coli ( E. coli ), wherein the viable E. coli is embedded in the matrix, and wherein the first polysaccharide is a hydrocolloid-forming polysaccharide, and
a second composition being different from the first composition, the second composition including a second polysaccharide and a disaccharide,
wherein the particle has a water activity (a w )≤0.3.
19 . The particle of claim 18 , wherein the first polysaccharide includes alginate.
20 . The particle of claim 18 , wherein 0.04≤a W ≤0.3.
21 . The particle of claim 18 , wherein said second composition comprises a ratio disaccharide/second polysaccharide of less than 10, wherein the ratio is wt. %/wt. %.
22 . The particle of claim 21 , wherein the ratio is of less than 5.
23 . The particle of claim 21 , wherein the ratio is of about 1.
24 . The particle of claim 18 , wherein said second composition further comprises a salt of L-glutamic acid.
25 . The particle of claim 24 , wherein said salt is sodium salt of L-glutamic acid.
26 . The particle of claim 18 , wherein said second polysaccharide includes maltodextrin.
27 . The particle of claim 18 , wherein said second polysaccharide includes dextran.
28 . The particle of claim 18 , wherein said E. coli includes a non-pathogenic E. coli.
29 . The particle of claim 18 , wherein said non-pathogenic E. coli includes the strain deposited at the International Depository Authority of Canada (IDAC) on Jan. 21, 2005, under accession number IDAC 210105-01.
30 . The particle of claim 18 , wherein said non-pathogenic E. coli includes the strain deposited at the International Depository Authority of Canada (IDAC) on Jun. 20, 2013, and attributed accession number 200613-01.
31 . The particle of claim 18 , wherein said hydrocolloid-forming polysaccharide is present in the matrix in percent by weight of total dry matter at a value of from 0.1% to 20%.
32 . The particle of claim 18 , wherein said hydrocolloid-forming polysaccharide is present in the matrix in percent by weight of total dry matter at a value of from 1 to 12%.
33 . The particle of claim 18 , wherein the first composition forms a core and the second composition is deposited on the core.
34 . The particle of claim 18 , wherein said second polysaccharide is dextran and said disaccharide is sucrose, present in a ratio of dextran:sucrose of from 10:1 to 1:10.