Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
1. A process for preparation of a sugar product from lignocellulosic material, comprising:
enzymatic hydrolysis of the lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;
whereby between 0.1 and 7.5 mg enzyme composition/g glucan on dry matter and enzyme as protein or between 0.05 and 3.0 mg enzyme composition/g feedstock on dry matter and enzyme as protein is used; and wherein:
the enzymatic hydrolysis occurs for a first part of time and a subsequent second part of time;
during the first part of time less or no oxygen is added to the lignocellulosic material compared to the second part of time, during the second part of time more oxygen is added to the lignocellulosic material compared to the first part of time, and the second part of time is 2% to 80% of total enzymatic hydrolysis time.
2. A process for preparation of a fermentation product from lignocellulosic material, comprising:
enzymatic hydrolysis of the lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61; whereby between 0.1 and 7.5 mg enzyme composition/g glucan on dry matter and enzyme as protein or between 0.05 and 3.0 mg enzyme composition/g feedstock on dry matter and enzyme as protein is used; and fermentation of the hydrolysed lignocellulosic material to produce a fermentation product; and wherein:
the enzymatic hydrolysis occurs for a first part of time and a subsequent second part of time;
during the first part of time less or no oxygen is added to the lignocellulosic material compared to the second part of time, during the second part of time more oxygen is added to the lignocellulosic material compared to the first part of time, and the second part of time is 2% to 80% of total enzymatic hydrolysis time.
3. The process according to claim 1 , wherein the oxygen is added to the lignocellulosic material in the form of bubbles.
4. The process according to claim 1 , wherein the enzymatic hydrolysis is performed in a reactor having a volume of 1 m 3 or more.
5. The process according to claim 1 , wherein enzymatic hydrolysis time is 5 to 150 hours.
6. The process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.
7. The process according to claim 1 , wherein the hydrolysis is conducted at a temperature of 45° C. or more.
8. The process according to claim 1 , wherein the enzyme composition is derived from a fungus, or the enzyme composition comprises a fungal enzyme.
9. The process according to claim 1 , wherein dry matter content in the enzymatic hydrolysis is 10 wt % or more.
10. The process according to claim 1 , wherein the dry matter content in the enzymatic hydrolysis is 14 to 33% wt %.
11. The process according to claim 1 wherein the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.
12. The process according to claim 1 wherein oxygen is introduced as air.
13. The process according to claim 8 , wherein the fungus is of the genus Rasamsonia , or the fungal enzyme is a Rasamsonia enzyme.
14. The process according to claim 1 , wherein the second part of time is 12% to 50% of total enzymatic hydrolysis time.
15. The process according to claim 1 , wherein during the first part of time dissolved oxygen in the lignocellulosic material is at a concentration that is at least 50% less than during the second part of time, and during the second part of time oxygen is added to the lignocellulosic material continuously.
16. The process according to claim 1 , wherein dissolved oxygen concentration in the lignocellulosic material is maintained in the range of 0.02 mol/m 3 to 0.045 mol/m 3 , and wherein the dissolved oxygen is measured under normal atmospheric pressure and at about 62° C.
17. The process according to claim 13 , wherein the fungus is Rasamsonia emersonii , or the fungal enzyme is a Rasamsonia emersonii enzyme.
18. The process according to claim 1 , wherein no oxygen is added to the lignocellulosic material during the first period of time.