Reaction processing vessel

1. A reaction processing vessel for processing a sample, comprising:

a substrate having a principal surface, and

a groove channel formed on the principal surface of the substrate, said groove channel comprising a first continuous serpiginous channel having a first end and a second end and a second continuous serpiginous channel having a first end and a second end,

wherein a plurality of reaction regions, each maintained at a predetermined temperature, are set at separate areas along the principal surface of the substrate, and two of said reaction regions are separated along the principal surface of the substrate by a third region,

wherein the sample repeatedly moves in a reciprocating manner between the plurality of reaction regions in order to cause a reaction,

wherein the channel comprises a separate serpiginous channel included in each of the plurality of reaction regions, and a connection channel connecting serpiginous channels in two adjacent regions of the plurality of reaction regions,

wherein the connection channel comprises a single detection channel portion adapted to be irradiated with excitation light in order to detect fluorescence from a sample flowing inside the channel,

wherein the serpiginous channel and the detection channel has an opening, a bottom surface, and side surfaces formed in a tapered shape expanding from the bottom surface toward the opening,

wherein the cross-sectional area of the detection channel is larger than the cross-sectional area of the serpiginous channel, and

wherein the bottom surface width of the detection channel is larger than the bottom surface width of the serpiginous channel.

2. The reaction processing vessel according to claim 1 , wherein given that the cross-sectional area of the serpiginous channel is denoted by Sr and that the cross-sectional area of the detection channel is denoted by Sd, a cross-sectional area ratio Sd/Sr is in a range of 1<Sd/Sr≤1.8.

3. The reaction processing vessel according to claim 2 , wherein the cross-sectional area ratio Sd/Sr is in a range of 1.02≤Sd/Sr≤1.5.

4. The reaction processing vessel according to claim 2 , wherein the cross-sectional area ratio Sd/Sr is in a range of 1.02≤Sd/Sr≤1.2.

5. The reaction processing vessel according to claim 1 ,

wherein the serpiginous channel has an opening width of 0.55 mm to 0.95 mm, a bottom surface width of 0 mm to 0.95 mm, a depth of 0.5 mm to 0.9 mm, and a taper angle of 0° to 45°, and

wherein the detection channel has an opening width of 0.7 mm to 1.2 mm, a bottom surface width of 0.15 mm to 1.2 mm, a depth of 0.5 mm to 1.2 mm, and a taper angle of 0° to 45°.

6. The reaction processing vessel according to claim 1 , wherein the bottom surface in the detection channel is formed on a flat surface parallel to the principal surface of the substrate.

7. The reaction processing vessel according to claim 6 , wherein connecting parts between the bottom surface and the side surfaces of the detection channel are formed in an angular shape.

8. The reaction processing vessel according to claim 6 , wherein the detection channel has a predetermined length that exceeds the bottom surface width of the detection channel in a direction along the connection channel.

9. The reaction processing vessel according to claim 1 , wherein the detection channel has a bottom surface width of 0.55 mm to 1.2 mm.

10. A reaction processing vessel for processing a sample, comprising:

a substrate having a principal surface, and

a groove channel formed on the principal surface of the substrate, said groove channel comprising a first continuous serpiginous channel having a first end and a second end and a second continuous serpiginous channel having a first end and a second end,

wherein a first temperature region maintained at a first temperature and a second temperature region maintained at a second temperature lower than the first temperature are set at separate areas along the principal surface of the substrate, and are separated by a third temperature region,

wherein the sample repeatedly moves in a reciprocating manner between the first temperature region and the second temperature region in order to cause a reaction,

wherein the first temperature region includes the first serpiginous channel,

wherein the second temperature region includes the second serpiginous channel,

wherein the third temperature region includes a single linear connection channel that is formed between one of the first and second ends of the first serpiginous channel and one of the first and second ends of the second serpiginous channel,

wherein a first linear braking channel for lowering a speed of the sample is formed at the other of the first and second ends of the first serpiginous channel,

wherein a second linear braking channel for lowering a speed of the sample is formed at the other of the first and second ends of the second serpiginous channel,

wherein the cross-sectional area of the first braking channel is larger than the cross-sectional area of the first serpiginous channel, and

wherein the cross-sectional area of the second braking channel is larger than the cross-sectional area of the second serpiginous channel.

11. The reaction processing vessel according to claim 10 , wherein given that the respective cross-sectional areas of the first serpiginous channel and the second serpiginous channel are denoted by Sr and that the respective cross-sectional areas of the first braking channel and the second braking channel are denoted by Sb, a cross-sectional area ratio Sb/Sr is in a range of 1<Sb/Sr≤1.8.

12. The reaction processing vessel according to claim 11 , wherein the cross-sectional area ratio Sb/Sr is in a range of 1.02≤Sb/Sr≤1.5.

13. The reaction processing vessel according to claim 11 , wherein the cross-sectional area ratio Sb/Sr is in a range of 1.02≤Sb/Sr≤1.2.

14. The reaction processing vessel according to claim 10 , wherein the first serpiginous channel, the second serpiginous channel, the first braking channel, and the second braking channel have an opening, a bottom surface, and side surfaces formed in a tapered shape expanding from the bottom surface toward the opening.

15. The reaction processing vessel according to claim 14 ,

wherein the first serpiginous channel and the second serpiginous channel have an opening width of 0.55 mm to 0.95 mm, a bottom surface width of 0 mm to 0.95 mm, a depth of 0.5 mm to 0.9 mm, and a taper angle of 0° to 45°, and

wherein the first braking channel and the second braking channel have an opening width of 0.65 mm to 1.05 mm, a bottom surface width of 0 mm to 1.05 mm, a depth of 0.5 mm to 0.9 mm, and a taper angle of 0° to 45°.

16. The reaction processing vessel according to claim 14 , wherein connecting parts between the bottom surface and the side surfaces have a curved surface.

17. The reaction processing vessel according to claim 16 , wherein the curvature radius of the connecting parts is 0.2 mm to 0.38 mm.

18. The reaction processing vessel according to claim 10 ,

wherein the first serpiginous channel and the second serpiginous channel include a bent part, and

wherein the curvature radius of the bent part is 0.3 mm to 10 mm.

19. The reaction processing vessel according to claim 10 , further comprising a branch channel branched from the channel and a sample introduction port provided in the branch channel,

wherein the distance between a temperature region closest to the branch channel and to the sample introduction port among the first temperature region and the second temperature region, and the branch channel and the sample introduction port is 5 mm or more.

20. The reaction processing vessel according to claim 10 , further comprising a pair of filters provided at the respective ends of the channel, a branch channel branched from the channel, and a sample introduction port provided in the branch channel,

wherein given that the volume of the channel from the sample introduction port to a filter closest to the sample introduction port is denoted by Vf and that the volume of the sample introduced from the sample introduction port is denoted by Vs, the following is satisfied: k×Vs<Vf (where k represents a real number of 0.1 to 10).

21. The reaction processing vessel according to claim 10 , further comprising a pair of filters provided at the respective ends of the channel, a branch channel branched from the channel, and a sample introduction port provided in the branch channel,

wherein, when the volume of the sample introduced from the sample introduction port is 1 μL to 50 μL, the length of the channel from the sample introduction port to a filter closest to the sample introduction port is 2 mm to 200 mm.

22. The reaction processing vessel according to claim 10 , wherein the first braking channel is formed within the first temperature region and the second braking channel is formed adjacent to and outside of the second temperature region.

23. The reaction processing vessel according to claim 10 , wherein the first and second temperature regions are heated regions and the third temperature region is an unheated region.

24. The reaction processing vessel according to claim 10 , wherein the groove channel comprises a first end and a second end, and a first filter disposed between said first end and said first braking channel, and a second filter disposed between said second end and said second braking channel.

25. A reaction processing vessel for processing a sample, comprising:

a substrate having a principal surface, and

a groove channel formed on the principal surface of the substrate, said groove channel comprising a first continuous serpiginous channel having a first end and a second end and a second continuous serpiginous channel having a first end and a second end,

wherein a first temperature region maintained at a first temperature and a second temperature region maintained at a second temperature lower than the first temperature are set at separate areas along the principal surface of the substrate, and are separated by a third temperature region,

wherein the sample repeatedly moves in a reciprocating manner between the first temperature region and the second temperature region in order to cause a reaction,

wherein the first temperature region includes the first serpiginous channel,

wherein the second temperature region includes the second serpiginous channel,

wherein the third temperature region includes a linear connection channel that is formed between one of the first and second ends of the first serpiginous channel and one of the first and second ends of the second serpiginous channel,

wherein a first linear braking channel for lowering a speed of the sample is formed at the other of the first and second ends of the first serpiginous channel,

wherein a second linear braking channel for lowering a speed of the sample is formed at the other of the first and second ends of the second serpiginous channel,

wherein the connection channel includes a detection channel portion that is adapted to be irradiated with excitation light in order to detect fluorescence from a sample flowing inside the channel, wherein the cross-sectional area of the first braking channel is larger than the cross-sectional area of the first serpiginous channel,

wherein the cross-sectional area of the second braking channel is larger than the cross-sectional area of the second serpiginous channel, and

wherein the cross-sectional area of the detection channel is larger than the cross-sectional area of the first serpiginous channel and the cross-sectional area of the second serpiginous channel.

26. The reaction processing vessel according to claim 25 , wherein the first braking channel is formed within the first temperature region and the second braking channel is formed adjacent to and outside of the second temperature region.

27. The reaction processing vessel according to claim 25 , wherein the first and second temperature regions are heated regions and the third temperature region is an unheated region.

28. The reaction processing vessel according to claim 25 , wherein the groove channel comprises a first end and a second end, and a first filter disposed between said first end and said first braking channel, and a second filter disposed between said second end and said second braking channel.