Freeze dried viral nanoparticle constructs
A method of producing plant virus-like particles includes freeze drying an aqueous solution of plant virus particles to produce a substantially RNA-free plant virus-like particles.
1. A method of producing plant virus-like particles, the method comprising:
freeze drying an aqueous solution of plant virus particles to produce substantially RNA-free plant virus-like particles, wherein the plant virus particles have an icosahedral structure.
2. The method of claim 1 , the aqueous solution of plant virus particles includes plant virus particles at a concentration of less than about 10 mg/ml.
3. The method of claim 1 , wherein the freeze drying includes freezing an aqueous solution of the plant virus particles at a temperature and for an amount of time that allows the plant virus particles to eject encapsulated genomic RNA while maintaining an intact capsid conformation.
4. The method of claim 3 , wherein the freeze drying includes heating the frozen aqueous solution at a temperature above freezing and at a pressure less than about 100 millibar to sublimate frozen water.
5. The method of claim 1 , further comprising contacting the freeze dried plant virus particles with RNase to degrade nucleic acid.
6. The method of claim 1 , wherein the plant virus particles comprise picornavirus particles.
7. The method of claim 6 , wherein the plant picornavirus particles are of the Comovirus genus.
8. The method of claim 6 , wherein the picornavirus comprising cowpea mosaic virus (CPMV).
9. A method of producing empty plant virus-like particles, the method comprising:
freezing an aqueous solution of plant virus particles at a temperature and for an amount of time that allows the plant virus particles to eject the encapsulated genomic RNA while maintaining an intact capsid conformation, wherein the plant virus particles have an icosahedral structure; and
sublimating the frozen aqueous solution of plant virus particles to remove water and RNA in the plant virus capsid.
10. The method of claim 9 , the aqueous solution of plant virus particles includes plant virus particles at a concentration of less than about 10 mg/ml.
11. The method of claim 9 , wherein the aqueous solution of the plant virus particles is frozen at a temperature of about −5° C. to about −40° C.
12. The method of claim 9 , wherein the step of sublimating the frozen aqueous solution includes heating the frozen aqueous solution at a temperature above freezing and at a pressure less than about 100 millibar.
13. The method of claim 9 , further comprising contacting the freeze dried plant virus particles with RNase to degrade nucleic acid.
14. The method of claim 9 , wherein the plant virus particles comprise picornavirus particles.
15. The method of claim 14 , wherein the plant picornavirus particles are of the Comovirus genus.
16. The method of claim 14 , the picornavirus comprising cowpea mosaic virus (CPMV).
17. A composition comprising a plurality of freeze dried, endoribonuclease treated plant virus-like particles prepared by the method of claim 1 ; wherein the plant virus-like particles have an icosahedral structure.
18. The composition of claim 17 , wherein during freeze drying an aqueous solution of icosahedral plant virus particles is frozen at a temperature and for an amount of time that allows the virus to eject encapsulated genomic RNA while maintaining an intact capsid conformation.
19. The composition of claim 17 , wherein freeze drying includes heating a frozen aqueous solution of icosahedral plant virus particles at a temperature above freezing and at a pressure less than about 100 millibar.
20. The composition of claim 17 , wherein the endoribonuclease comprises RNaseA.
21. The composition of claim 17 , wherein the plant virus-like particles comprise cowpea mosaic virus-like particles.