Capillary electrophoresis inkjet dispensing
Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.
1. A method for dispensing an analyte from a capillary electrophoresis tube, the method comprising:
applying a voltage potential through a capillary electrophoresis tube, the capillary electrophoresis tube having a capillary outlet, and a capillary longitudinal axis proximate to the capillary outlet;
impulsively pumping a sheath liquid through a microfluidic pump chamber having a internal region that is configured to be deformed by an impulsive pump element, wherein the capillary electrophoresis tube extends through the internal region of the microfluidic pump chamber, the microfluidic pump chamber being in fluidic connection with a microfluidic nozzle, the microfluidic nozzle having a nozzle outlet, a tapered internal region proximate to the nozzle outlet, and a nozzle longitudinal axis proximate to the nozzle outlet, wherein the capillary outlet of the capillary electrophoresis tube is located within the tapered internal region of the microfluidic nozzle;
mixing a separated analyte with the sheath liquid, wherein the separated analyte exits the capillary electrophoresis tube through the capillary outlet, and the mixing of the separated analyte and the sheath liquid is substantially entirely within the tapered internal region of the microfluidic nozzle; and
dispensing the mixture of the separated analyte and the sheath liquid through the nozzle outlet of the microfluidic nozzle.
2. The method of claim 1 , wherein the capillary electrophoresis tube is at least partially filled with a separation buffer or a sieving matrix.
3. The method of claim 1 wherein the method further comprises:
controlling the pressure of the sheath liquid in a sheath liquid reservoir, the sheath liquid reservoir in fluidic connection with the microfluidic pump chamber.
4. The method of claim 1 wherein the method further comprises:
controlling the pressure of a capillary electrophoresis solution in a capillary electrophoresis solution reservoir, the capillary electrophoresis solution reservoir in fluidic connection with the capillary outlet.
5. The method of claim 1 wherein the method further comprises:
flowing a capillary electrophoresis solution through the capillary electrophoresis tube and out of the capillary outlet, wherein the flowing is subsequent to applying the voltage potential.
6. The method of claim 1 wherein the capillary longitudinal axis of the capillary electrophoresis tube is parallel to the nozzle longitudinal axis of the microfluidic nozzle.
7. The method of claim 1 wherein the capillary longitudinal axis of the capillary electrophoresis tube extends through the nozzle outlet of the microfluidic nozzle.
8. The method of claim 1 wherein the capillary longitudinal axis of the capillary electrophoresis tube is coaxial with the nozzle longitudinal axis of the microfluidic nozzle.
9. The method of claim 1 wherein the dispensing of the mixture out of the nozzle creates one or more droplets.
10. The method of claim 1 wherein the dispensing of the mixture out of the nozzle creates a stream.
11. The method of claim 1 wherein the dispensing further comprises contacting the dispensed mixture with a surface.
12. The method of claim 11 wherein the surface comprises a hydrophobic material.
13. The method of claim 11 wherein the surface comprises a hydrophilic material.
14. The method of claim 11 wherein the surface is a blotting membrane.
15. The method of claim 11 further comprising:
moving the surface relative to the microfluidic nozzle.
16. The method of claim 11 further comprising:
moving the microfluidic nozzle relative to the surface.
17. The method of claim 1 wherein the capillary outlet terminates between about 5 μm and about 500 μm from the nozzle outlet.
18. The method of claim 1 wherein a diameter or a major axis of the nozzle outlet is between about 5 μm and about 200 μm.
19. The method of claim 1 wherein the impulsive pump element comprises a piezoelectric material or a thermoresistive material.
20. The method of claim 1 wherein the capillary longitudinal axis of the capillary electrophoresis tube is angled with respect to the nozzle longitudinal axis of the microfluidic nozzle.