Compositions and methods for storing liquid biospecimens
A lyoprotectant composition includes one or more lyoprotective carbohydrates comprising a non-reducing polysaccharide. The non-reducing polysaccharide may be dextranol. The one or more lyoprotective carbohydrates may include a non-reducing disaccharide. The lyoprotectant composition may be free of or substantially free of reducing sugars. The composition may be in the form of a matrix including a non-woven web of fibers having an average diameter of 0.1 μm to 2.5 μm. A method of storing a liquid biospecimen includes mixing the liquid biospecimen with the lyoprotectant composition to prepare a specimen-loaded composition; drying the specimen-loaded composition; and storing the dried specimen-loaded composition.
1. A lyoprotectant matrix comprising:
a non-woven web of fibers comprising:
a non-reducing polysaccharide comprising dextranol; and
a non-reducing mono- or disaccharide,
the fibers having an average diameter of 0.1 μm to 2.5 μm.
2. The lyoprotectant matrix of claim 1 , wherein the non-reducing mono- or disaccharide comprises trehalose.
3. The lyoprotectant matrix of claim 1 , wherein all carbohydrates in the lyoprotectant matrix are non-reducing sugars.
4. The lyoprotectant matrix of claim 1 , wherein the lyoprotectant matrix is free of or substantially free of reducing sugars.
5. The lyoprotectant matrix of claim 1 , wherein the lyoprotectant matrix is free of or substantially free of dextran.
6. The lyoprotectant matrix of claim 1 , wherein the lyoprotectant matrix is free of or substantially free of compounds comprising aldehyde and ketone groups.
7. The lyoprotectant matrix of claim 1 , wherein the lyoprotectant matrix further comprises one or more excipients.
8. The lyoprotectant matrix of claim 7 , wherein the excipient increases lyoprotection of at least one biomarker of interest.
9. The lyoprotectant matrix of claim 7 , wherein the excipient comprises an amino acid, an antioxidant, a polymer, a carbohydrate, or a bulking agent.
10. The lyoprotectant matrix of claim 7 , wherein the excipient comprises glycerol, polyethylene glycol (PEG), polysorbate 20, gluconic acid, or glucamine.
11. The lyoprotectant matrix of claim 1 , wherein the dextranol has a concentration of from 0.1 g/mL to 5 g/mL.
12. A method of storing a liquid biospecimen, the method comprising:
mixing the liquid biospecimen with a lyoprotectant composition comprising a non-reducing polysaccharide to prepare a specimen-loaded composition, the non-reducing polysaccharide comprising dextranol;
drying the specimen-loaded composition; and
storing the dried specimen-loaded composition.
13. The method of claim 12 , wherein the liquid biospecimen comprises a purified protein.
14. The method of claim 12 , wherein the liquid biospecimen comprises an enzyme.
15. The method of claim 12 , wherein the liquid biospecimen comprises whole blood, serum, saliva, or urine.
16. The method of claim 12 , wherein the storing comprises storing at a temperature of 4° C. or greater.
17. The method of claim 12 , wherein the storing comprises storing at a temperature of 22° C. or greater.
18. The method of claim 12 , wherein the storing comprises storing for a time period of one week or greater.
19. The method of claim 12 , wherein the storing comprises storing for a time period of two weeks or greater.
20. The method of claim 12 , the method further comprising transporting the dried specimen-loaded composition.