Peptide-polynucleotide-hyaluronic acid nanoparticles and methods for polynucleotide transfection
The present disclosure relates to nanoparticles and methods for polynucleotide transfection.
1. A pharmaceutical composition comprising a peptide-polynucleotide complex, the peptide-polynucleotide complex comprising:
a peptide;
a polynucleotide; and
hyaluronic acid;
wherein the peptide is non-lytic and capable of affecting the release of a polynucleotide from an endosome of a cell; and
wherein the peptide comprises SEQ ID NO: 1.
2. The composition of claim 1 , wherein the peptide-polynucleotide complex is about 10 nm to about 150 nm in diameter.
3. The composition of claim 2 , wherein the peptide-polynucleotide complex is about 10 nm to about 50 nm in diameter.
4. The composition of claim 1 , wherein the hyaluronic acid coats the peptide-polynucleotide complex.
5. The composition of claim 1 , wherein the hyaluronic acid is integrated into the peptide-polynucleotide complex.
6. The composition of claim 1 , wherein the polynucleotide is an RNA.
7. The composition of claim 1 , wherein the polynucleotide is an mRNA.
8. The composition of claim 1 , wherein the polynucleotide is a non-coding RNA capable of regulating or inhibiting the expression of a nucleic acid sequence.
9. The composition of claim 8 , wherein the non-coding RNA is an siRNA.
10. The composition of claim 8 , wherein the non-coding RNA is an miRNA.
11. The composition of claim 1 , wherein the peptide consists of SEQ ID NO: 1.
12. The composition of claim 1 , wherein the peptide-polynucleotide complex comprises a ratio of peptide:polynucleotide that is more than about 50:1 and less than about 200:1.