Composition and method for generating immunity to
Provided is an immunogenic composition including a peptide, wherein consecutive amino acids of the peptide include at least amino acids 186-193 of SEQ ID NO:1 and one or more adjuvants. In an example, the peptide is covalently linked to an amino acid sequence including SEQ ID NO:2. Also provided is a method of vaccinating a subject against Borrelia burgdorferi , including administering to the subject an effective amount of the immunogenic composition.
1. An immunogenic composition, comprising
a peptide, wherein consecutive amino acids of the peptide comprise consecutive amino acids of SEQ ID NO:1 and the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-193 of SEQ ID NO:1, 187-194 of SEQ ID NO:1, 188-195 of SEQ ID NO:1, 189-196 of SEQ ID NO:1, and any combination of two or more of the foregoing, and
one or more adjuvants, wherein at least one of the one or more adjuvants is selected from an aluminum salt, AS04, AS03, monophosphoryl lipid A, poly(I:C), a CpG DNA adjuvant, MF59, an emulsion adjuvant comprising squalene and water, a combination adjuvant comprising block copolymer CRL-8300, squalene, a sorbitan monooleateor, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium salt (DOTAP), 3 β-[N—(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-chol liposome), and a virosomal adjuvant.
2. The immunogenic composition of claim 1 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-218 of SEQ ID NO:1, 187-218 of SEQ ID NO:1, 188-218 of SEQ ID NO:1, 189-218 of SEQ ID NO:1, and any combination of two or more of the foregoing.
3. The immunogenic composition of claim 1 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 1-193 of SEQ ID NO:1, 1-194 of SEQ ID NO:1, 1-195 of SEQ ID NO:1, 1-196 of SEQ ID NO:1, and any combination of two or more of the foregoing.
4. The immunogenic composition of claim 1 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-222 of SEQ ID NO:1, 187-222 of SEQ ID NO:1, 188-222 of SEQ ID NO:1, 189-222 of SEQ ID NO:1, and any combination of two or more of the foregoing.
5. The immunogenic composition of claim 1 , wherein the peptide comprises amino acids 1-218 of SEQ ID NO:1.
6. The immunogenic composition of claim 1 , wherein the peptide comprises SEQ ID NO:1.
7. The immunogenic composition of claim 1 , wherein at least one of the one or more adjuvants is an aluminum salt.
8. The immunogenic composition of claim 1 , wherein at least one of the one or more adjuvants is a CpG DNA adjuvant.
9. The immunogenic composition of claim 1 , wherein at least one of the one or more adjuvants is a virosomal adjuvant.
10. A method of vaccinating a subject against Borrelia burgdorferi , comprising
administering to the subject an effective amount of an immunogenic composition comprising a peptide and one or more adjuvants,
wherein consecutive amino acids of the peptide comprise consecutive amino acids of SEQ ID NO:1 and the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-193 of SEQ ID NO:1, 187-194 of SEQ ID NO:1, 188-195 of SEQ ID NO:1, 189-196 of SEQ ID NO:1, and any combination of two or more of the foregoing,
at least one of the one or more adjuvants is selected from an aluminum salt, AS04, AS03, monophosphoryl lipid A, poly(I:C), a CpG DNA adjuvant, MF59, an emulsion adjuvant comprising squalene and water, a combination adjuvant comprising block copolymer CRL-8300, squalene, a sorbitan monooleateor, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium salt (DOTAP), 3 β-[N—(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-chol liposome), and a virosomal adjuvant, and
the subject is a mammal.
11. The method of claim 10 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-218 of SEQ ID NO:1, 187-218 of SEQ ID NO:1, 188-218 of SEQ ID NO:1, 189-218 of SEQ ID NO:1, and any combination of two or more of the foregoing.
12. The method of claim 10 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 1-193 of SEQ ID NO:1, 1-194 of SEQ ID NO:1, 1-195 of SEQ ID NO:1, 1-196 of SEQ ID NO:1, and any combination of two or more of the foregoing.
13. The method of claim 10 , wherein the consecutive amino acids of SEQ ID NO:1 are selected from the group consisting of amino acids 186-222 of SEQ ID NO:1, 187-222 of SEQ ID NO:1, 188-222 of SEQ ID NO:1, 189-222 of SEQ ID NO:1, and any combination of two or more of the foregoing.
14. The method of claim 10 , wherein the peptide comprises amino acids 1-218 of SEQ ID NO:1.
15. The method of claim 10 , wherein the peptide comprises SEQ ID NO:1.
16. The method of claim 10 , wherein at least one of the one or more adjuvants is an aluminum salt.
17. The method of claim 10 , wherein at least one of the one or more adjuvants is a CpG DNA adjuvant.
18. The method of claim 10 , wherein at least one of the one or more adjuvants is a virosomal adjuvant.
19. The method of claim 10 , wherein the subject is selected from a human, a cervid, and a rodent.
20. The method of claim 10 , wherein the subject is a mouse.
21. The method of claim 10 , wherein administering comprises oral administration.