Polypeptides including a beta-tricalcium phosphate-binding sequence and uses thereof
Provided herein are beta-TCP binding sequences and chimeric polypeptides that include one or more beta-TCP binding sequences and a mammalian growth factor, compositions comprising any of these chimeric polypeptides (and optionally, beta-TCP), and methods of promoting bone or cartilage formulation, methods of replacing and/or repairing bone or cartilage, and methods of treating a bone fraction or bone loss that include administration of any of these compositions.
1. A polypeptide comprising: (a) a sequence at least 80% identical to SEQ ID NO: 2 (VIGESTHHRPWS), (b) a sequence at least 80% identical to SEQ ID NO: 4 (ILAESTHHKPWT), (c) a sequence at least 80% identical to SEQ ID NO: 6 (IIGESSHHKPFT), (d) a sequence at least 80% identical to SEQ ID NO: 7 (GLGDTTHHRPWG), or (e) a combination comprising two or more sequences of (a)-(d).
2. The polypeptide of claim 1 , comprising a sequence at least 90% identical to SEQ ID NO: 2.
3. The polypeptide of claim 2 , comprising SEQ ID NO: 2.
4. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 2, and the sequence at least 80% identical to SEQ ID NO: 4.
5. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 2, and the sequence at least 80% identical to SEQ ID NO: 6.
6. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 2, and the sequence at least 80% identical to SEQ ID NO: 7.
7. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 2, and a sequence at least 80% identical to SEQ ID NO: 1 (LLADTTHHRPWT).
8. The polypeptide of claim 1 , comprising a sequence at least 90% identical to SEQ ID NO: 4.
9. The polypeptide of claim 8 , comprising SEQ ID NO: 4.
10. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 4, and the sequence at least 80% identical to SEQ ID NO: 6.
11. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 4, and the sequence at least 80% identical to SEQ ID NO: 7.
12. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 4, and a sequence at least 80% identical to SEQ ID NO: 1 (LLADTTHHRPWT).
13. The polypeptide of claim 1 , comprising a sequence at least 90% identical to SEQ ID NO: 6.
14. The polypeptide of claim 13 , comprising SEQ ID NO: 6.
15. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 6, and the sequence at least 80% identical to SEQ ID NO: 7.
16. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 6, and a sequence at least 80% identical to SEQ ID NO: 1 (LLADTTHHRPWT).
17. The polypeptide of claim 1 , comprising a sequence at least 90% identical to SEQ ID NO: 7.
18. The polypeptide of claim 17 , comprising SEQ ID NO: 7.
19. The polypeptide of claim 1 , comprising a combination of the sequence at least 80% identical to SEQ ID NO: 7, and a sequence at least 80% identical to SEQ ID NO: 1 (LLADTTHHRPWT).
20. The polypeptide of claim 1 , further comprising a sequence at least 80% identical to SEQ ID NO: 1 (LLADTTHHRPWT).