Recombinant expression of
The present invention relates to methods for the recombinant expression of chlamydia major outer membrane protein (MOMP) comprising transforming a population of E. coli host cells with an expression vector comprising a nucleic acid molecule that encodes chlamydia MOMP and encodes a leader sequence for targeting the MOMP to the outer membrane of the cell, wherein the nucleic acid molecule is operatively linked to a promoter. The method of the invention allows expression of MOMP in the outer membrane of the cell, which leads to protein folding that is more like native MOMP relative to a MOMP protein that is expressed intracellularly. Also provided by the invention are uses of the recombinant MOMP in pharmaceutical compositions and methods for the treatment and/or prophylaxis of chlamydia infection and/or the effects thereof.
1. A nucleic acid molecule comprising a sequence of nucleotides as set forth in SEQ ID NO:15, SEQ ID NO:18, or SEQ ID NO:21.
2. The nucleic acid molecule of claim 1 , comprising a sequence of nucleotides as set forth in SEQ ID NO:15.
3. The nucleic acid molecule of claim 1 , comprising a sequence of nucleotides as set forth in SEQ ID NO:18.
4. The nucleic acid molecule of claim 1 , comprising a sequence of nucleotides as set forth in SEQ ID NO:21.
5. A nucleic acid molecule comprising a nucleic acid derivative having at least 85% sequence identity to a sequence of nucleotides set forth in SEQ ID NO 15, SEQ ID NO: 18, or SEQ ID NO: 21, wherein the nucleic acid molecule retains the leader sequence.
6. A nucleic acid molecule comprising a nucleic acid derivative having at least 90% sequence identity to a sequence of nucleotides set forth in SEQ ID NO 15, SEQ ID NO: 18, or SEQ ID NO: 21, wherein the nucleic acid molecule retains the leader sequence.
7. A nucleic acid molecule comprising a nucleic acid derivative having at least 95% sequence identity to a sequence of nucleotides set forth in SEQ ID NO 15, SEQ ID NO: 18, or SEQ ID NO: 21, wherein the nucleic acid molecule retains the leader sequence.
8. A nucleic acid molecule comprising a nucleic acid derivative having at least 97% sequence identity to a sequence of nucleotides set forth in SEQ ID NO 15, SEQ ID NO: 18, or SEQ ID NO: 21, wherein the nucleic acid molecule retains the leader sequence.
9. A nucleic acid molecule comprising a nucleic acid derivative having at least 99% sequence identity to a sequence of nucleotides set forth in SEQ ID NO 15, SEQ ID NO: 18, or SEQ ID NO: 21, wherein the nucleic acid molecule retains the leader sequence.