G- protein coupled receptor extraction formulations
Formulations for extraction and stabilization of G-Protein Coupled Receptors (GPCRs), kits and methods for their use for extracting GPCRs from biological specimens (e.g., cells and tissues), and methods for making such formulations in bulk scale are described. GPCRs extracted from cells and tissues using the described formulations remain in a functional, solubilized form over extended time periods.
1. A bulk scale solution comprising a solubilized cholesterol derivative, comprising:
a maltoside-based non-ionic detergent;
a zwitterionic detergent;
a buffering agent, wherein the buffering agent is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), MOPS (3-(N-morpholino)propanesulfonic acid), MES (2-(N-morpholino)ethanesulfonic acid), HEPPs, or PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid));
10-20% (w/v) of a polyol; and
about 0.15 to 0.3% (w/v) of a cholesterol derivative, wherein the concentration ratio (w/v) of the cholesterol derivative to total detergent is about 0.5 or less, and wherein the solution is at a pH of 5-8, and wherein the cholesterol derivative remains solubilized in the bulk scale solution, wherein the bulk scale solution has a volume of about 1 L to about 20 L.
2. A method of extracting a membrane protein from a cell or a tissue, comprising:
providing a sample comprising a cell or a tissue; and
incubating the sample in the solution of claim 1 , wherein the solution is in an amount sufficient to extract the membrane protein from the cell or tissue, and wherein the extracted membrane protein is solubilized in the solution.
3. The method of claim 2 , wherein the membrane protein is a G-protein coupled receptor (GPCR), and wherein the solubilized GPCR maintains functional integrity.
4. The method of claim 3 , wherein greater than 70% of the function of the GPCR is maintained when stored for 7 days at 4° C. or wherein greater than 70% of the function of the GPCR is maintained when stored for 1 month at −20° C.
5. A kit for extracting a GPCR from a cell or tissue, comprising:
a) a GPCR extraction solution, comprising:
a maltoside-based non-ionic detergent;
a zwitterionic detergent;
a buffering agent, wherein the buffering agent is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), MOPS (3-(N-morpholino)propanesulfonic acid), MES (2-(N-morpholino)ethanesulfonic acid), HEPPs, or PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid));
10-20% (w/v) of a polyol; and
about 0.15 to 0.3% (w/v) of a cholesterol derivative, wherein the concentration ratio (w/v) of the cholesterol derivative to total detergent is about 0.5 or less, and wherein the solution is at a pH of 5-8, and wherein the cholesterol derivative remains solubilized in the solution; and
b) instructions for extracting the GPCR from the cell or tissue.
6. A method for preparing a solution in bulk scale according to claim 1 , wherein the solution comprises solubilized cholesterol derivative, comprising:
combining the maltoside-based non-ionic detergent and the zwitterionic detergent to provide a solution at a first temperature;
adding the buffering agent into the solution;
elevating the temperature of the solution to a second temperature, wherein the second temperature is greater than the first temperature;
mixing the cholesterol derivative into the solution at the second temperature; and
adding the polyol into the solution to provide the bulk scale solution, wherein the bulk scale solution is buffered to pH 5-8, and wherein the cholesterol derivative is solubilized in the bulk scale solution.
7. The method of claim 6 , further comprising returning the bulk scale solution to a third temperature that is less than the second temperature, wherein the cholesterol derivative is solubilized in the bulk scale solution at the third temperature.
8. The solution of claim 1 , wherein the non-ionic detergent is selected from n-dodecyl-b-D-maltopyranoside, n-octyl-β-D-maltopyranoside, n-hexyl-β-D-maltopyranoside, glyco-diosgenin, lauryl maltose neopentyl glycol, and maltose neopentyl glycol.
9. The solution of claim 1 , wherein the zwitterionic detergent is 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), cocamidopropyl hydroxysultaine, cocamidopropyl betaine, phospholipids, phosphatidylethanolamine, or phosphatidylcholine.
10. The solution of claim 1 , wherein the cholesterol derivative is an acidic cholesterol ester.
11. The solution of claim 1 , wherein the cholesterol derivative is selected from cholesteryl hemisuccinate (CHS), cholesterol α-D-glucopyranosyl-(1→4)-β-D-glucopryanoside, and 5,11,17-tris[(carboxy)methyl]-25-monomethoxytriazolo-(3α-cholesteryl)-26,27,28-trihydroxycalix[4]arene.
12. The solution of claim 1 , wherein the polyol is glycerol, sorbitol, or xylitol.
13. The solution of claim 1 , wherein the solution is at a pH of 7-7.5.
14. A solution comprising a solubilized cholesterol derivative, wherein the solution comprises:
a maltoside-based non-ionic detergent;
a zwitterionic detergent;
a buffering agent, wherein the buffering agent is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), MOPS (3-(N-morpholino)propanesulfonic acid), MES (2-(N-morpholino)ethanesulfonic acid), HEPPs, or PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid));
10-20% (w/v) of a polyol; and
about 0.15 to 0.3% (w/v) of a cholesterol derivative, wherein the concentration ratio (w/v) of the cholesterol derivative to total detergent is about 0.5 or less, and wherein the solution is at a pH of 5-8, wherein the cholesterol derivative remains solubilized in the solution.
15. The solution of claim 14 , wherein the non-ionic detergent is selected from n-dodecyl-b-D-maltopyranoside, n-octyl-β-D-maltopyranoside, n-hexyl-β-D-maltopyranoside, glyco-diosgenin, lauryl maltose neopentyl glycol, and maltose neopentyl glycol.
16. The solution of claim 14 , wherein the zwitterionic detergent is selected from 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), cocamidopropyl hydroxysultaine, cocamidopropyl betaine, phospholipids, phosphatidylethanolamine, and phosphatidylcholine.
17. The solution of claim 14 , wherein the cholesterol derivative is selected from cholesteryl hemisuccinate (CHS), cholesterol α-D-glucopyranosyl-(1→4)-β-D-glucopryanoside, and 5,11,17-tris[(carboxy)methyl]-25-monomethoxytriazolo-(3α-cholesteryl)-26,27,28-trihydroxycalix[4]arene.
18. The solution of claim 14 , wherein the polyol is glycerol, sorbitol, or xylitol.
19. A method of extracting a membrane protein from a cell or a tissue, comprising:
providing a sample comprising a cell or a tissue; and
incubating the sample in the solution of claim 14 , wherein the solution is in an amount sufficient to extract the membrane protein from the cell or tissue, and wherein the extracted membrane protein is solubilized in the solution.
20. The method of claim 19 , wherein the non-ionic detergent is selected from n-dodecyl-b-D-maltopyranoside, n-octyl-β-D-maltopyranoside, n-hexyl-β-D-maltopyranoside, glyco-diosgenin, lauryl maltose neopentyl glycol, and maltose neopentyl glycol, and wherein the zwitterionic detergent is selected from 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), cocamidopropyl hydroxysultaine, cocamidopropyl betaine, phospholipids, phosphatidylethanolamine, and phosphatidylcholine.