Methods and apparatuses for sorting target particles
This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting.
1. A method of sorting cells, comprising screening cellular material to identify cells with a desired phenotype wherein the cellular material is screened at a rate of 1,000,000 cells per second or greater.
2. A method of sorting cells, comprising screening cellular material to identify cells with a desired phenotype, wherein the cellular material is screened at a rate of 50,000 cells per second or greater; and
extracting said cells of a desired phenotype from said cellular material at a rate of 100,000 cells per second or greater.
3. The method of claim 2 , wherein said cells of a desired phenotype are extracted at a rate of 300,000 cells per second or greater.
4. A method of sorting cells, comprising screening cellular material to identify cells with a desired phenotype, wherein the cellular material is screened at a rate of 50,000 cells per second or greater; and wherein said cellular material is screened in an array with through-holes.
5. The method of claim 4 , wherein said cells of said desired phenotype are extracted from said array.
6. The method of claim 5 , wherein said extracting comprises releasing said cells of said desired phenotype using electromagnetic radiation.
7. The method of claim 6 , wherein each through-hole of said array comprises from 0 to about 5 cells and at least 30% of the through-holes of the array comprise at least one cell.
8. The method of claim 1 , wherein the cellular material is obtained from a human subject and/or comprises less than 5% of HSCs and HSPCs.
9. The method of claim 2 , wherein greater than 95% of the extracted cells are HSCs and/or HSPCs.
10. The method of claim 2 , wherein greater that 95% of the extracted cells are cells of said desired phenotype.
11. The method of claim 2 , wherein greater than 95% of the extracted cells are viable as determined by evaluating the cells within 5 hours from said extracting.
12. The method of claim 2 , wherein the extracted cells are suitable for therapeutic use without the need for additional sterilization steps.
13. The method of claim 2 , wherein the extracted cells are essentially free of pathogens.
14. The method of claim 2 , wherein the extracted cells comprise less than 0.1% of pathogens.
15. The extracted cells obtained by the method of claim 2 .
16. A pharmaceutical composition comprising the extracted cells of claim 15 .