Preservation of cell-free nucleic acids
A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.
1. A composition for the stabilization of nucleic acids within a plasma sample comprising:
a. an amount of free formaldehyde;
b. about 200 to 500 g/L of one or more preservative agents including diazolidinyl urea;
c. a solvent including water;
wherein upon mixing the composition with a blood sample and storing the blood sample at room temperature for at least 7 days, cell-free RNA detection is improved due to reduced cellular RNA presence and a reduction in a decrease in cell-free RNA as a result of the composition.
2. The composition of claim 1 , wherein the one or more preservative agents includes imidazolidinyl urea.
3. The composition of claim 1 , further comprising one or more nuclease inhibitors that include an anticoagulant.
4. The composition of claim 2 , further comprising one or more nuclease inhibitors that include an anticoagulant.
5. The composition of claim 3 , wherein the one or more nuclease inhibitors includes EDTA.
6. The composition of claim 4 , wherein the one or more nuclease inhibitors includes EDTA.
7. The composition of claim 1 , wherein the one or more preservative agents includes imidazolidinyl urea, dimethyoylol-5,5dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane-1,3-diol, oxazolidines, sodium hydroxymethyl glycinate, 5-hydroxymethoxymethyl-1-1aza-3,7-dioxabicyclo [3.3.0]octane, 5-hydroxymethyl-1-1aza-3,7dioxabicyclo[3.3.0]octane, quaternary adamantine and combinations thereof.
8. The composition of claim 5 , wherein the one or more preservative agents includes imidazolidinyl urea, dimethoylol-5,5dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane-1,3-diol, oxazolidines, sodium hydroxymethyl glycinate, 5-hydroxymethoxymethyl-1-1aza-3,7-dioxabicyclo [3.3.0]octane, 5-hydroxymethyl-1-1aza-3,7dioxabicyclo[3.3.0]octane, 5-hydroxypoly[methyleneoxy]methyl-1-1aza-3,7dioxabicyclo[3.3.0]octane, quaternary adamantine and any combination thereof.
9. The composition of claim 1 , wherein the concentration of the one or more preservative agents is about 200 g/l to about 400 g/l.
10. The composition of claim 6 , wherein the concentration of the one or more preservative agents is about 200 g/l to about 400 g/l.
11. The composition of claim 8 , wherein the concentration of the one or more preservative agents is about 200 g/l to about 400 g/l.
12. The composition of claim 1 , wherein the composition is mixed with a blood sample so that the amount of the one or more preservative agents is less than 10 g/l of the blood sample.
13. The composition of claim 2 , wherein the composition is mixed with a blood sample so that the amount of the one or more preservative agents is less than 10 g/l of the blood sample.
14. The composition of claim 7 , wherein the composition is mixed with a blood sample so that the amount of the one or more preservative agents is less than 10 g/l of the blood sample.
15. The composition of claim 1 , wherein the composition is located into a tube having an interior wall that is flame sprayed, subjected to corona discharge, plasma treated, coated or otherwise treated.
16. The composition of claim 2 , wherein the composition is located into a tube having an interior wall that is flame sprayed, subjected to corona discharge, plasma treated, coated or otherwise treated.
17. The composition of claim 7 , wherein the composition is located into a tube having an interior wall that is flame sprayed, subjected to corona discharge, plasma treated, coated or otherwise treated.
18. The composition of claim 15 , wherein the interior wall includes a coating that is sprayed, dipped, swabbed, or otherwise applied onto some or all of the interior wall of the tube.
19. The composition of claim 16 , wherein the interior wall includes a coating that is sprayed, dipped, swabbed, or otherwise applied onto some or all of the interior wall of the tube.
20. The composition of claim 1 , wherein the composition is located into a tube that is glass.