PepCon proteomics standards and methods of use
Described are methods, compositions, and devices for a concatemeric protein standard that behaves as a protein but transforms into single peptides upon digestion, which is optimized to function as a non-obtrusive process control for mass spectrometry analysis.
1. A peptide concatemer (“PepCon”) comprising two or more copies of a single, non-natural peptide linked by a cleavage site, wherein the peptide comprises the sequence set forth in SEQ ID NO. 7 or a variant thereof which is at least 80% homologous to SEQ ID NO. 7.
2. The PepCon of claim 1 , further comprising an affinity tag.
3. The PepCon of claim 2 , wherein the affinity tag is a FLAG, HA, His, myc, chitin binding protein (CBP), maltose binding protein (MBP), or glutathione-S-transferase (GST) tag.
4. The PepCon of claim 1 , further comprising a secretory signal peptide.
5. The PepCon of claim 4 , wherein the secretory signal peptide is a prokaryotic secretory signal peptide.
6. The PepCon of claim 5 , wherein the prokaryotic secretory signal peptide is a Lpp, LamB, LTB, MalE, OmpA, OmpC, OmpF, OmpT, PelB, PhoA, PhoE, or SpA peptide.
7. The PepCon of claim 1 , wherein the cleavage site is a protease cleavage site.
8. The PepCon of claim 7 , wherein the protease cleavage site is an aminopeptidase M, bromelain, carboxypeptidase A, carboxypeptidase B, carboxypeptidase P, carboxypeptidase Y, cathepsin C, chymotrypsin, collagenase, dispase, elastase, endoproteinase Arg-C, endoproteinase Asp-N, endoproteinase Glu-C, endoproteinase Lys-C, enterokinase, factor Xa, ficin, human rhinovirus (HRV) 3C protease (or its GST fusion, PreScission protease), kallikrein, papain, pepsin, plasmin, pronase, proteinase K, subtilisin, TEV, thermolysin, thrombin, or trypsin cleavage site.
9. The PepCon of claim 7 , wherein upon digestion at the protease cleavage site, the PepCon generates the two or more copies of the peptide.
10. The PepCon of claim 1 , wherein the peptide is optimized for protein solubility or electrospray ionization (“ESI”).
11. The PepCon of claim 1 , wherein the PepCon comprises 15 or more copies of the single peptide.
12. The PepCon of claim 1 , wherein the PepCon comprises 30 or more copies of the single peptide.
13. A peptide concatemer (“PepCon”) having the sequence set forth in SEQ ID NO. 4 or SEQ ID NO. 10.