Soybean event DP-305423-1 and compositions and methods for the identification and/or detection thereof
Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.
1. A recombinant polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO:5, nucleotides 12,164-14,494 of SEQ ID NO:6, nucleotides 5,751-7,813 of SEQ ID NO:7, nucleotides 2,899-7,909 of SEQ ID NO:82, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, or SEQ ID NO:88, or a full complement thereof.
2. The recombinant polynucleotide of claim 1 , comprising the nucleotide sequence of SEQ ID NO: 5, 6, 7, or 82, or a full complement thereof.
3. The recombinant polynucleotide of claim 1 , comprising the nucleotide sequence of SEQ ID NO: 8, 9, 14, 15, 20, 21, 83 or 84, or a full complement thereof.
4. A pair of DNA molecules consisting of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein the DNA molecules have a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO: 5, 6, 7, or 82, or a complete complement thereof, and wherein the first DNA molecule resides in a transgene insert DNA sequence of SEQ ID NO: 5, 6, 7, or 82, and the second DNA molecule resides in the corresponding soy genomic DNA sequence of SEQ ID NO: 5, 6, 7, or 82, to function as DNA primers when used together in an amplification reaction with a template from soybean event DP-305423-1 to produce an amplicon diagnostic for soybean event DP-305423-1 in a sample, and wherein the amplicon comprises the nucleotide sequences of SEQ ID NO: 8, 9, 14, 15, 20, 21, 83 or 84, or full complements thereof.
5. The pair of DNA molecules of claim 4 , wherein the amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 20 and SEQ ID NO: 21, or the full complements thereof.
6. The pair of DNA molecules of claim 4 , wherein the amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 83 and SEQ ID NO: 84, or the full complements thereof.
7. The pair of DNA molecules of claim 4 , wherein the DNA molecules are at least 18 nucleotides in length.
8. The pair of DNA molecules of claim 7 , wherein the DNA molecules are at least 24 nucleotides in length.
9. The pair of DNA molecules of claim 8 , wherein the DNA molecules are at least 30 nucleotides in length.
10. The pair of DNA molecules of claim 4 , wherein at least one of the pair of DNA molecules comprises a nucleotide sequence selected from SEQ ID NO: 26, 27, 29, 30, 31, 32, 33, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, and 81.
11. A DNA detection kit comprising the pair of DNA molecules of claim 4 .
12. A DNA detection kit comprising a pair of DNA molecules consisting of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein the DNA molecules have a nucleotide sequence of at least 18 contiguous nucleotides of SEQ ID NO: 5, 6, 7, or 82, or a complete complement thereof, and wherein the first DNA molecule resides in a transgene insert DNA sequence of SEQ ID NO: 5, 6, 7, or 82, and the second DNA molecule resides in the corresponding soy genomic DNA sequence of SEQ ID NO: 5, 6, 7, or 82, to function as DNA primers when used together in an amplification reaction with a template from soybean event DP-305423-1 to produce an amplicon diagnostic for soybean event DP-305423-1 in a sample, and wherein the amplicon comprises the nucleotide sequences of SEQ ID NO: 8, 9, 14, 15, 20, 21, 83 or 84, or the full complements thereof.
13. The DNA detection kit of claim 12 , wherein the amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 20 and SEQ ID NO: 21, or the full complements thereof.
14. The DNA detection kit of claim 12 , wherein the amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 83 and SEQ ID NO: 84, or the full complements thereof.
15. The DNA detection kit of claim 12 , wherein the DNA molecules are at least 24 nucleotides in length.
16. The DNA detection kit of claim 12 , wherein the DNA molecules are at least 30 nucleotides in length.
17. The DNA detection kit of claim 12 , wherein at least one of the pair of DNA molecules comprises a nucleotide sequence selected from SEQ ID NO: 26, 27, 29, 30, 31, 32, 33, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, and 81.