Nucleic acid based data storage
Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.
1. A method for storing information, the method comprising:
(a) converting at least one item of information in a form of at least one digital sequence to at least one nucleic acid sequence;
(b) synthesizing a plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence, wherein each polynucleotide comprises:
(i) a plurality of coding regions, wherein each coding region is identical; and
(ii) at least one non-coding region, wherein the at least one non-coding region comprises a cleavage region; and
(c) storing the plurality of polynucleotides.
2. The method of claim 1 , wherein the cleavage region comprises a restriction enzyme recognition site.
3. The method of claim 1 , wherein the cleavage region comprises a light sensitive nucleobase.
4. The method of claim 1 , further comprising application of a restriction enzyme, electromagnetic radiation, or a gaseous reagent to cleave at the cleavage region, thereby removing at least one of the plurality of coding regions.
5. The method of claim 1 , wherein each coding region comprises 25 to 500 bases in length.
6. The method of claim 1 , wherein each coding region comprises 10 to 2000 bases in length.
7. The method of claim 1 , wherein each non-coding region comprises 1 to 10 bases in length.
8. The method of claim 1 , wherein each non-coding region comprises at most 200 bases in length.
9. The method of claim 1 , wherein the plurality of polynucleotides comprises at least 10,000 polynucleotides.
10. The method of claim 1 , wherein the plurality of polynucleotides comprises at least 1 billion polynucleotides.
11. The method of claim 1 , wherein greater than 90% of the polynucleotides encode for a sequence that does not differ from the predetermined sequence.
12. The method of claim 1 , wherein the at least one item of information is text information, audio information or visual information.
13. The method of claim 1 , wherein a first non-coding region within each polynucleotide has a different sequence than a second non-coding region within each polynucleotide.
14. The method of claim 1 , wherein each non-coding region within each polynucleotide has a different sequence.
15. The method of claim 1 , wherein a first cleavage region within each polynucleotide has a different sequence than a second cleavage region within each polynucleotide.
16. The method of claim 1 , wherein each cleavage region within each polynucleotide has a different sequence.
17. The method of claim 1 , wherein a number of cleavage regions within each polynucleotide is at least 1, 2, 3, 4, or 5.
18. The method of claim 17 , wherein each cleavage region within each polynucleotide has a different sequence.
19. The method of claim 1 , wherein each polynucleotide comprises a tether region.
20. The method of claim 1 , further comprising selectively transferring the plurality of polynucleotides to a receiving unit, wherein selectively transferring comprises application of a force, wherein the force is laminar pressure, capillary pressure, slip flow pressure, magnetic force, electrostatic force, peristaltic force, sound waves, vibrational force, centripetal force, centrifugal force, or any combination thereof.