Patent
This invention relates to methods and compositions for providing a benefit to a plant by associating the plant with a beneficial endophyte of the genus Streptomyces , including benefits to a plant derived from a seed or other plant element treated with said endophyte. For example, this invention provides purified endophytes, synthetic combinations comprising endophytes, and methods of making and using the same. In particular, this invention relates to compositions and methods of improving soybean and maize plants.
1. A method of enhancing symbiosis in a plant grown from a plant reproductive element, comprising treating the plant reproductive element with a formulation comprising an effective amount of a Streptomyces endophyte that is heterologous to the plant reproductive element, wherein the Streptomyces endophyte comprises a 16S polynucleotide sequence having at least 97% identity to SEQ ID NO 2; and at least 3 polynucleotides that encode an arabinose transporter protein;
wherein the effective amount is effective to increase expression of one or more genes encoding a sugar transporter domain in a plant grown from the treated plant reproductive element, as compared to an isoline plant grown from a plant reproductive element not treated with the Streptomyces endophyte.
2. The method of claim 1 , wherein the plant is soybean.
3. The method of claim 1 , wherein the plant reproductive element is a seed.
4. The method of claim 3 , wherein the seed is a transgenic seed.
5. The method of claim 1 , wherein the formulation comprises a purified population of the Streptomyces endophyte at a concentration of at least about 10{circumflex over ( )}2 CFU/ml in a liquid formulation or about 10{circumflex over ( )}2 CFU/gm in a non-liquid formulation.
6. The method of claim 1 , wherein the formulation further comprises one or more of the following: stabilizer, preservative, carrier, surfactant, anticomplex agent, fungicide, nematicide, bactericide, insecticide, herbicide, or any combination thereof.
7. The method of claim 1 , wherein the treating comprises coating the plant reproductive element with the formulation, spraying the formulation onto the plant reproductive element, or introducing the formulation onto a soil comprising the plant reproductive element.
8. A method of decreasing abundance of one or more bacteria of the genus Escherichia - Shigella in leaves of a plant under water-stressed conditions, comprising treating a plant reproductive element with a formulation comprising an effective amount of a Streptomyces endophyte that is heterologous to the plant reproductive element, wherein the Streptomyces endophyte comprises a 16S polynucleotide sequence having at least 97% identity to SEQ ID NO: 2 and at least 3 polynucleotides that encode an arabinose transporter protein,
wherein the effective amount is effective to decrease abundance of one or more bacteria of the genus Escherichia - Shigella in leaves of a plant grown from the treated reproductive element under water-stressed conditions, as compared to an isoline plant grown from a plant reproductive element not treated with the Streptomyces endophyte.
9. The method of claim 8 , wherein the plant is soybean.
10. The method of claim 8 , wherein the plant reproductive element is a seed.
11. The method of claim 10 , wherein the seed is a transgenic seed.
12. The method of claim 8 , wherein the formulation comprises a purified population of the Streptomyces endophyte at a concentration of at least about 10{circumflex over ( )}2 CFU/ml in a liquid formulation or about 10{circumflex over ( )}2 CFU/gm in a non-liquid formulation.
13. The method of claim 8 , wherein the formulation further comprises one or more of the following: stabilizer, preservative, carrier, surfactant, anticomplex agent, fungicide, nematicide, bactericide, insecticide, herbicide, or any combination thereof.
14. The method of claim 8 , wherein the treating comprises coating the plant reproductive element with the formulation, spraying the formulation onto the plant reproductive element, or introducing the formulation onto a soil comprising the plant reproductive element.
15. A synthetic composition comprising a plant reproductive element with a formulation comprising a purified Streptomyces endophyte population applied thereon, wherein the Streptomyces endophyte is heterologous to the plant reproductive element and comprises a 16S polynucleotide sequence having at least 97% identity to SEQ ID NO: 2; and at least 3 polynucleotides that encode an arabinose transporter protein;
wherein the endophyte is present in the synthetic composition in an amount capable of conferring to a plant at least one plant trait selected from: increased expression of one or more genes encoding a sugar transporter domain, and decreased abundance of one or more bacteria of the genus Escherichia - Shigella in leaves of the plant under water-stressed conditions; as compared to an isoline plant grown from a plant reproductive element not treated with the Streptomyces endophyte.
16. The composition of claim 15 , wherein the plant is soybean.
17. The composition of claim 15 , wherein the plant reproductive element is a seed.
18. The composition of claim 17 , wherein the seed is a transgenic seed.
19. The composition of claim 15 , wherein the formulation comprises a purified population of the Streptomyces endophyte at a concentration of at least about 10{circumflex over ( )}2 CFU/ml in a liquid formulation or about 10{circumflex over ( )}2 CFU/gm in a non-liquid formulation.
20. The composition of claim 15 , wherein the formulation further comprises one or more of the following: stabilizer, preservative, carrier, surfactant, anticomplex agent, fungicide, nematicide, bactericide, insecticide, herbicide, or any combination thereof.
21. The composition of claim 15 , wherein the 16S polynucleotide sequence has least 98% identity to SEQ ID NO: 2.
22. The composition of claim 15 , wherein the 16S polynucleotide sequence has least 99% identity to SEQ ID NO: 2.
23. The composition of claim 15 , wherein the 16S polynucleotide sequence comprises the sequence set forth in SEQ ID NO: 2.
24. The method of claim 8 , wherein the 16S polynucleotide sequence has least 98% identity to SEQ ID NO: 2.
25. The method of claim 8 , wherein the 16S polynucleotide sequence has least 99% identity to SEQ ID NO: 2.
26. The method of claim 8 , wherein the 16S polynucleotide sequence comprises the sequence set forth in SEQ ID NO: 2.