Granted Patent B2

US 12,274,993 · App. 16/932,392 · Granted Apr 15, 2025

Inventors: Timothy Manning Swager (Newton, MA); Jie Li (Waltham, MA); Suchol Savagatrup (Cambridge, MA); Zachary P. Nelson (Cambridge, MA); Kosuke Yoshinaga (Cambridge, MA); Cassandra Zentner (Brighton, MA)

Assignee: Massachusetts Institute of Technology

B01J13/14B01J13/046C01B33/145G01N33/5432

Abstract

Claims (16)

1. A system comprising:

a plurality of Janus droplets comprising an interface between a first phase and a second phase of the droplets, the second phase immiscible with the first phase; and

a binding moiety to a biological analyte associated with the plurality of Janus droplets,

wherein the first phase comprises an emissive species that interacts with electromagnetic radiation in a detectable manner,

wherein the second phase comprises a blocking species that at least partially absorbs and/or reflects electromagnetic radiation such that the blocking species at least partially blocks the passage of electromagnetic radiation that interacts with the first phase and/or at least partially blocks the passage of electromagnetic radiation emitted by the first phase,

wherein the plurality of Janus droplets have a first orientation in the absence of the biological analyte and a second orientation upon binding of the binding moiety to the biological analyte, such that the electromagnetic radiation emitted or transmitted in the second orientation is less than in the first orientation.

2. A system as in claim 1 , wherein the plurality of Janus droplets is constructed and arranged to produce an electromagnetic radiation signal indicative of the presence and/or amount of the biological analyte exposed to the droplets.

3. A system as in claim 2 , wherein the plurality of Janus droplets is constructed and arranged to change in orientation and/or morphology when exposed to the analyte in a manner that affects an electromagnetic radiation signal, determined by the detector.

4. A system as in claim 1 , wherein the second phase contains an emissive species that emits light at a different wavelength than the emissive species in the first phase.

5. A system as in claim 1 , further comprising at least one surfactant stabilizing the interface between the first and second phase and/or between the first phase and/or the second phase and a continuous phase in which the droplets are provided, wherein the at least one surfactant can respond to the presence of the analyte in a way that changes the structure of the droplet.

6. A system as in claim 5 , wherein an analyte can cause a change in emission detectable with respect to alignment of the droplet and changes in the detected emission indicate the presence of an analyte.

7. A system as in claim 5 , comprising at least one magnetic material associated with at least one droplet, optionally added to the droplet.

8. A system as in claim 5 , comprising an emissive species comprising a sub-phthalocyanine dye, a perylene dye, or a phthalocyanine dye.

9. A system as in claim 5 , wherein, upon exposure of the system to a chemical or biological analyte, the system generates the determinable signal.

10. A system as in claim 5 , comprising a first emissive species associated with the first phase and a second emissive species associated with the second phase.

11. A system as in claim 1 , wherein a peak wavelength of emission of the emissive species and a peak wavelength of blockage of the blocking species differ by no more than 200 nanometers in wavelength.

Assignments (2)

Continuity (2)

References Cited (218)