High throughput BH3 profiling: a rapid and scalable technology to BH3 profile on low numbers of cells
The present invention relates provides methods of predicting cell sensitivity to a test agent. In some embodiments, the cells are cultured in a culture medium having serum.
1. A multi-well plate comprising a plurality of wells coated with an adhesive agent, wherein at least one well of the plurality comprises a test therapeutic agent, a BH3 domain peptide, and a cell culture in a culture medium having serum.
2. The multi-well plate of claim 1 , wherein the BH3 domain peptide is derived from the BH3 domain of a BID, a BIM, a BAD, a NOXA, a PUMA, a BMF, or an HRK polypeptide.
3. The multi-well plate of claim 1 , wherein the BH3 domain peptide is selected from the group consisting of SEQ ID NO: 1-15.
4. The multi-well plate of claim 1 , wherein the test therapeutic agent is a chemotherapeutic agent.
5. The multi-well plate of claim 1 , wherein the adhesive agent is an extracellular matrix (ECM) protein.
6. The multi-well plate of claim 5 , wherein the ECM protein is selected from the group consisting of collagen 1, laminin, collagen 4 and fibronectin.
7. The multi-well plate of claims 1 , wherein the adhesive agent is an antibody.
8. A kit comprising:
a multi-well plate for automated drug screening, the multi-well plate comprising an array of at least 384 wells, wherein a plurality of wells in the array are coated with an adhesive agent, wherein at least one well of the plurality comprises a test therapeutic agent and a BH3 domain peptide; and
a vial containing a BH3 profiling buffer at a concentration of 2×, 3×, 4×, or 5×,
wherein the BH3 profiling buffer is a Derived from Trehalose Experimental Buffer (DTEB) at a concentration of 2×, 3×, 4×, or 5×, wherein a 1× concentration of DTEB comprises: 135 mM trehalose, 10 mM HEPES, 50 mM KCl, 0.02 mM EGTA, 0.02 mM EDTA, 0.1% BSA, and 5 mM succinate, or
wherein the BH3 profiling buffer is a Mannitol Experimental Buffer (MEB) at a concentration of 2×, 3×, 4×, or 5×, wherein a 1× concentration of MEB comprises: 150 mM mannitol, 10 mM HEPES, 50 mM KCl, 0.02 mM EGTA, 0.02 mM EDTA, 0.1% BSA, and 5 mM succinate.
9. The kit of claim 8 , wherein the BH3 domain peptide is derived from the BH3 domain of a BID, a BIM, a BAD, a NOXA, a PUMA, a BMF, or an HRK polypeptide.
10. The kit of claim 8 , wherein the BH3 domain peptide is selected from the group consisting of SEQ ID NO: 1-15.
11. The kit of claim 8 , wherein the test therapeutic agent is a chemotherapeutic agent.
12. The kit of claim 11 , wherein the chemotherapeutic agent is a small molecule, a peptide, a protein, or an RNA-interference (RNAi) molecule.
13. The kit of claim 8 , wherein the adhesive agent is an extracellular matrix (ECM) protein.
14. The kit of claim 13 , wherein the ECM protein is selected from the group consisting of collagen 1, laminin, collagen 4 and fibronectin.
15. The kit of claim 8 , wherein the adhesive agent is an antibody.
16. The kit of claim 8 , wherein the BH3 profiling buffer is supplemented with a permeabilizing agent.
17. The kit of claim 8 , further comprising a potentiometric dye.
18. The kit of claim 17 , wherein the potentiometric dye is 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), dihydrorhodamine 123, tetramethylrhodamine methyl ester (TMRM), or tetramethylrhodamine ethyl ester (TMRE).
19. The kit of claim 8 , further comprising an antibody for cytochrome c, SMAC/Diablo, Omi, adenylate kinase-2, or apoptosis-inducing factor.