Anti-bed bug monoclonal antibodies and methods of making and uses thereof
The present disclosure provides anti-bed bug monoclonal antibodies and antigen-binding fragments thereof as well as compositions and kits comprising the same. The present disclosure also provides methods of making monoclonal antibodies and antigen-binding fragments thereof and methods of using the same to detect bed bugs.
1. A conjugated monoclonal antibody or a conjugated antigen-binding fragment, comprising the three heavy and three light chain complementarity determining regions of an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], and a detection agent.
2. The conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 , comprising the heavy and light chain variable regions of the antibody produced by the hybridoma.
3. The conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 , comprising the heavy and light chains of the antibody produced by the hybridoma.
4. The conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 , wherein the detection agent comprises an enzymatic label, a radiolabel, a fluorophore, a chromophore, an imaging agent, a metal, a metal ion, colloidal gold, or gold nanoparticles.
5. A conjugated monoclonal antibody or a conjugated antigen-binding fragment comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2], or an antigen-binding fragment thereof, and a detection agent comprising an enzymatic label, a radiolabel, a fluorophore, a chromophore, an imaging agent, a metal, a metal ion, colloidal gold, or gold nanoparticles.
6. A conjugated monoclonal antibody or a conjugated antigen-binding fragment comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645 [BB7], or an antigen-binding fragment thereof, and a detection agent comprising an enzymatic label, a radiolabel, a fluorophore, a chromophore, an imaging agent, a metal, a metal ion, colloidal gold, or gold nanoparticles.
7. A composition comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 .
8. The composition of claim 7 , further comprising an antibody or an antigen-binding fragment comprising the three heavy and three light chain complementarity determining regions of an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7].
9. A composition comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 5 .
10. The composition of claim 9 , further comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
11. A composition comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 6 .
12. The composition of claim 11 , further comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
13. A kit comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 .
14. The kit of claim 13 , further comprising an antibody or an antigen-binding fragment comprising the three heavy and three light chain complementarity determining regions of an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7].
15. A kit comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 5 .
16. The kit of claim 15 , further comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
17. A kit comprising the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 6 .
18. The kit of claim 17 , further comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
19. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 1 , and detecting binding of the bed bug antigen to the conjugated monoclonal antibody or conjugated antigen-binding fragment.
20. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 5 , and detecting binding of the bed bug antigen to the conjugated monoclonal antibody or conjugated antigen-binding fragment.
21. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with the conjugated monoclonal antibody or conjugated antigen-binding fragment of claim 6 , and detecting binding of the bed bug antigen to the conjugated monoclonal antibody or conjugated antigen-binding fragment.