IP Library Granted Patent US 11,988,659
Granted Patent B2
US 11,988,659 · App. 16/948,372 · Granted May 21, 2024

Nanopore arrays

Inventors: Roger J. A. Chen (Saratoga, CA); David J. Fullagar (Los Gatos, CA)
Assignee: Roche Sequencing Solutions, Inc.
G01N33/48721G11C7/00G11C16/34
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 11,988,659
App. No.
16/948,372
Granted
May 21, 2024
Kind
B2
Abstract

A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

Claims (19)

1. A system for analyzing a plurality of molecules, the system comprising:

a nanopore array including a plurality of cells included on a chip;

a fluidic system configured to send one or more reagents to the nanopore array;

a data interface configured to receive measurements from the plurality of cells; and

a master controller, the master controller programmed to:

assemble lipid bilayers in the plurality of cells;

insert nanopores into the assembled lipid bilayers;

determine states of the plurality of cells based on measurements from the plurality of cells;

analyze a sample using the inserted nanopores;

after analyzing the sample; direct the fluidic system to rinse out the contents within the cells;

assemble new lipid bilayers in the plurality of cells;

insert nanopores into the new lipid bilayers;

determine states of the plurality of cells with the new lipid bilayers based on measurements from the plurality of cells; and

analyze a second sample using the nanopores inserted into the new lipid bilayers.

2. The system of claim 1 , further comprising a memory that stores information about the states of the plurality of cells.

3. The system of claim 1 , wherein the master controller is further configured to determine when to receive measurements from the nanopore array.

4. The system of claim 3 , wherein at least a portion of the measurements from the nanopore array is used to analyze the molecules.

5. The system of claim 1 , wherein a state for each cell includes one of the following: a lipid bilayer is not formed, a lipid bilayer is formed, and a nanopore is formed.

6. The system of claim 1 , wherein the one or more reagents include the plurality of molecules.

Assignments (1)
MERGER AND CHANGE OF NAME Recorded Mar 16, 2022
From: GENIA TECHNOLOGIES, INC.; ROCHE SEQUENCING SOLUTIONS, INC
To: ROCHE SEQUENCING SOLUTIONS, INC.
Reel/Frame 059287/0200 →
Continuity (4)
Continuation 15983426 · May 18, 2018
Continuation 15462483 · Mar 17, 2017
Continuation 13759701 · Feb 5, 2013
Related Publication 20200408736A1 · Dec 31, 2020