Process for the preparation of pickering emulsion forming particles by derivatization of cellulose-rich dietary fibers with enzymes and emulsions prepared
The present invention relates to a process for forming a functionalised dietary fibre comprising admixing an enzyme and an aqueous suspension of dietary fibre, wherein said dietary fibre is at a D 50 particle size distribution of less than 30 microns after degradation by the enzyme and comprises less than 25 wt. % soluble fibres and at least 40% wt. % cellulose; denaturing said enzyme to form a functionalised, amphiphilic dietary fibre with adsorbed enzyme. The present invention further relates to a Pickering particle comprising a functionalised dietary fibre and denatured enzyme and the use of the functionalised dietary fibre and denatured enzyme according to present invention or the Pickering particle according to the present invention to stabilize an emulsion.
1. A process for forming a functionalised and amphiphilic dietary fiber, the process comprising:
admixing an enzyme and an aqueous suspension of dietary fiber selected from the group consisting of pea hull fiber, pulse hulls, bamboo fiber, cocoa fibers and coffee fibers, wherein the dietary fiber has a D 50 particle size distribution of less than 30 microns after degradation by the enzyme, and the dietary fiber comprises less than 25 wt. % soluble fibers and at least 40 wt. % cellulose; and
denaturing the enzyme to form the functionalised and amphiphilic dietary fiber with the denatured enzyme adsorbed on a surface of the functionalised and amphiphilic dietary fiber.
2. The process according to claim 1 further comprising hydrolysing the dietary fiber with the enzyme for a period up to 24 hours after the admixing of the enzyme and the aqueous suspension of the dietary fiber.
3. The process according to claim 1 , wherein the enzyme comprises one or more enzymes selected from the group consisting of endo-1,4-β-glucanase, cellobiohydrolase, β-glucosidase, endo-polygalacturonase, exo-polygalacturonase, pectin-methyl-esterase, endo-pectate lyase, exo-pectate lyase and pectin lyase.
4. The process according to claim 1 , wherein the dietary fiber comprises pea hull fiber.