METHOD FOR STABILIZING EXTRACELLULAR VESICLES
The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.
1 . A method of stabilizing an extracellular vesicle, the method comprising mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.
2 . The method of claim 1 , wherein the extracellular vesicle is an exosome.
3 . The method of claim 1 , wherein the extracellular vesicle-containing sample is a body fluid or a culture supernatant.
4 . The method of claim 1 , wherein the extracellular vesicle-containing sample is a blood sample.
5 . The method of claim 1 , wherein a concentration of the sacchande in the mixing is 2.5 to 100 mg/mL.
6 . The method of claim 1 , wherein a concentration of the chelating agent in the mixing is 1 to 200 mM.
7 . A method of stabilizing an extracellular vesicle, the method comprising:
(1) mixing an extracellular vesicle-containing sample with a saccharide, to obtain a mixture; and
(2) freezing the mixture.
8 . The method of claim 7 , wherein the freezing comprises freeze-drying.
9 . A method of stabilizing an extracellular vesicle, the method comprising:
(1) mixing an extracellular vesicle-containing sample with a chelating agent, to obtain a mixture; and
(2) freezing the mixture.
10 . The method of claim 9 , wherein the freezing comprises freeze-drying.
11 - 12 . (canceled)
13 . A stabilized extracellular vesicle obtained by the method of claim 1 .
14 . A stabilized extracellular vesicle obtained by the method of claim 7 .
15 . A stabilized extracellular vesicle obtained by the method of claim 9 .