Oligonucleotide conjugate with high hybridization performance and use thereof
The present invention provides an oligonucleotide conjugate with high hybridization performance and use thereof. The oligonucleotide conjugate of the present invention can be used as a probe for nucleic acid detection, the oligonucleotide conjugate of the present invention can be used to design probes more flexibly, and the conservative regions are more accessible. The specificity of the probe is better, and the fluorescence value of the probe can be significantly improved, the fluorescence background is significantly reduced, and the sensitivity of the probe is greatly improved.
1. An oligonucleotide conjugate, wherein the structure of the oligonucleotide conjugate is shown in the following Formula I:
R—N-Q-D, I
wherein R is an optional fluorescent group, N is an oligonucleotide unit, Q is a quenching group, and D is a cationic unit.
2. The oligonucleotide conjugate of claim 1 , wherein N is an oligonucleotide sequence of 3-60 bases.
3. The oligonucleotide conjugate of claim 2 , wherein N is an oligonucleotide sequence of 8-40 bases.
4. The oligonucleotide conjugate of claim 1 , wherein the cationic unit D contains one or more amino groups.
5. The oligonucleotide conjugate of claim 1 , wherein D is an organic cationic unit of a mer, wherein a is an integer between 1 and 30.
6. The oligonucleotide conjugate of claim 5 , wherein the organic cationic unit is spermine.
7. The oligonucleotide conjugate of claim 5 , wherein a is an integer between 2 and 20.
8. The oligonucleotide conjugate of claim 7 , wherein a is an integer between 3 and 10.
9. The oligonucleotide conjugate of claim 1 , wherein in Formula I, the 5′ end of N is connected R, and the 3′ end of N is connected to Q.
10. The oligonucleotide conjugate of claim 1 , wherein in Formula I, the 3′ end of N is connected to R, and the 5′ end of N is connected to Q.
11. A kit comprising the oligonucleotide conjugate of claim 1 .
12. A PCR reaction system, comprising the oligonucleotide conjugate of claim 1 .
13. The PCR reaction system of claim 12 , wherein the PCR reaction system includes a probe made from the oligonucleotide conjugate of claim 1 .
14. A method for real-time fluorescence quantitative PCR, wherein the method comprises the steps of:
(1) providing a nucleic acid sample of a subject to be tested;
(2) preparing a PCR reaction system and performing PCR detection:
wherein the PCR reaction system comprises: the nucleic acid sample provided in step (1), and the oligonucleotide conjugate of claim 1 .